查询词典 digitonin

The docked cell was perforated in the electrophoretic buffer containing digitonin and was lysed under a direct current electric field.

单个细胞用液压导入到双T的交界面，在电泳缓冲液中毛地黄皂苷的作用下，细胞膜被穿孔。

The results showed that 6% was the best concentration of separation gel for PSI complex separation. Part of PSI and amount of thylakoid membrane fragments contained with PSI complex could be solubilized by low concentration of Digitonin.

结果表明：6%的分离胶对PSI具有较好的分离特性；低浓度的毛地黄皂苷能够增溶间质类囊体上的PSI蛋白复合物，并能够获得大量的含有PSI的类囊体膜碎片。

Methods: Human lung epithelial cell line A549 treated with or without digitonin were stimulated by bacterial supernatant and sonicated bacterial extract from Klebsiella pneumoniae respectively.

实验细胞分为两组，一组用能使细胞膜通透性增加的温和去污剂digitonin处理，另一组不处理。

All PSI complexes could be solubilized by high concentration of Digitonin. Different area of thylakoid membrane could be separated by Tween-20 and PSI complex could be found in them solubilized by Digitonin.

高浓度的毛地黄皂苷能够获得全部的PSI复合物；Tween-20能够分离不同区域的类囊体膜，结合毛地黄皂苷处理均能够获得PSI复合物。

Methods The human pancreatic cancer Panc-1 cells were exposed to a single fraction of high-doseγ-ray radiation either with CMNa or under hypoxic condition. The percentage of dead cells was detected with a multiwell plated reader, and fluorescence intensities of propidium iodide were measured before and after digitonin treatment.

在乏氧和加不同浓度CMNa环境下对体外培养的人胰腺癌Panc-1细胞进行单次大剂量的γ射线照射，通过微孔板荧光酶标仪检测细胞死亡率来研究放射增敏作用。

The chlorophyll protein complexes of the thylakoid membranes solubilized with high concentration of digitonin combined sonication are isolated by means of nondenatured DOC-PAGE, and their biochemical and photochemical properties examined.

采用含PEG保护剂的提取介质提取类囊体，高浓度毛地黄皂苷与超声波破碎类囊体膜，非变性DOC-PAGE分离色素蛋白复合物。

By releasing the cytosol proteins with Digitonin and centrifugation technology, we divided the J6-2 cell proteins into the cytosol proteins and the particulate-conjugated proteins, and then using SDS-PAGE, Western blotting and Enzyme-linked immunosorbent stain combined with computer quantiscan technology, we investigated the effect of GM3 on PLC1 translocation.

本文采用洋地黄皂甙增加细胞膜渗透性，释放胞液蛋白，通过离心将细胞蛋白分为胞液蛋白和颗粒结合蛋白两部分，然后采用SDS-PAGE，Western blot及酶联免疫显色技术，并结合计算机扫描半定量技术，检测了GM3处理前后PLC1在细胞内的转位情况。

Sonicated bacterial extract caused a modest, less than 1 fold, increase in IL-8 secretion by digitonin-untreated A549 cells. In the digitonin- permeabilized A549 cells,Sonicated bacterial extract strongly induced the expression of IL-8,a 3 fold increase over the control.

经digitonin处理使细胞膜通透性增加后，Kp培养上清及菌体成分刺激细胞IL-8的作用都增强，菌体成分刺激IL-8效果更为显著，为对照的3倍，而培养上清作用相对较弱。

Digitonin is a galactoside.

洋地黄皂甘是一种半乳糖甘。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失