查询词典 digestions

The results showed that the total numbers of integral membrane proteins identified based on PAOT,trypsin/chymotrypsin and methanol-modified digestions were 101,59 and 72,which included 67,40 and 31 proteins with GRAVY＞0,respectively.

结果表明，甲醇酶解法鉴定到72个整合膜蛋白，其中平均疏水值大于0的蛋白数为31；胰蛋白酶/胰凝乳蛋白酶酶解法共鉴定到59个整合膜蛋白，其中平均疏水值大于0的蛋白数为40个；PAOT法共鉴定到101个整合膜蛋白，其中平均疏水值大于0的蛋白数为67个。

A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence. The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物，经过扩增前导序列10~34aa基因序列，并重新克隆入质粒pRSET-A构建串联二聚体后，再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点，经一系列简单的酶切和连接，快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因，并成功表达其六聚体重组蛋白。

New method that Biological Tissue Section tryptic digestions in situ coupled with imaging mass spectrometry was established.

通过正交设计实验，优化实验条件，我们建立了在福尔马林固定组织切片上加胰蛋白酶液(0.1ug/ul)，60℃超声10分钟进行修复提取，再直接质谱扫描分析的新方法。

Methods After enzymatic digestions of rat testis,the suspension passed the BSA uncontinuous gradient medium in a gravity unit by velocity sedimentation.

方法酶消化法制备大鼠睾丸组织单细胞悬液，采用牛血清白蛋白不连续密度梯度沉降法分离Sertoli细胞和精原细胞。

With templates originating from 7 digestions, a 2228-bp 5'-upstream sequence of a cotton small GTPase gene was obtained by two rounds of sequential YADE amplifications.

利用该方法通过2轮连续的扩增从7种酶切连接产物中成功地获得了1个棉花小GTP酶基因的2228bp上游序列。

The calcium soap stays inert in the rumen and then passes on through to the abomasums where the relative acidity condition there will naturally break down the calcium bonding and allow for optimal intestinal digestions.

钙皂延缓了饲料在瘤胃中的惰性，然后直到进入皱胃，在那里相对较酸的环境将自然地分解钙连接，且消化力也最佳。

In the city ecosystem, the wastes created in the production and the life, often need different decompositions and digestions.

在城市生态系统中，生产与生活中产生的废弃物往往需要异地分解与消化。

Some of 'em grieved till they got poor digestions and couldn't relish what they did have.

有的'时间悲痛，直至他们得到消化差，不能津津乐道他们的确有。

Results Insertion of gene and the recombination of adenovirus were identified by restrict enzyme digestions and PCR.

结果经限制性酶切和PCR扩增鉴定，证实为复制缺陷型BMP-2重组腺病毒。

DNA efficiency and quality can be analysed by agarose gel electrophoresis and double enzymatic digestions.

结果 C、D法提纯的质粒DNA的含量、纯度和收获率均较A、B高，上述4 方法提纯的质粒DNA都能进行酶切反应。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。