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PCR-RFLP in FSHR gene 5'–flanking region with exon 1 digested by restriction endonuclease TaqⅠdemonstrated that the frequencies of allele A (0.5500) and genotype AA (0.5000) in twinning cows were higher than that of allele A (0.3500) and genotype AA (0.3000) in monovular cows for Qinchuan cattle, while The frequencies of allele B (0.5937) and genotype BB(0.6875) in twinning cows were higher than that of allele B (0.5416) and genotype BB (0.4167) in monovular cows for Holstein cattle. Key words: Bovine; Twinning; FSHR gene; PCR-RFLP

FSHR基因5'端（包括侧翼序列和第一外显子）的限制性内切酶TaqⅠ的PCR-RFLP标记研究发现，在秦川牛中双胎母牛的A基因频率（0.5500）和AA基因型频率（0.5000）分别高于单胎母牛的A基因频率（0.3500）和AA基因型频率（0.3000）；在黑白花奶牛中双胎母牛的B基因频率（0.5937）和BB基因型频率（0.6875）均高于单胎母牛的B基因频率（0.5416）和BB基因型频率（0.4167），也就是FSHR基因的5'端的PCR-RFLP标记在一个品种内单胎母牛和双胎母牛之间有一定趋势，但两个品种的趋势不同。

Hexon protein gene of egg drop syndrome virus, named strain E05 which was isolated from breeding muscovy duck with irregular egg dropping was amplified by PCR and cloned into pMD18-T vector. The recombinant plasmids were identified by digested restriction endonuclease and PCR. The result showed that the nucleotide length of hexon protein gene encoding 910 amino acids was 2733 bp.

运用PCR技术从表现产蛋异常的种番鸭分离鉴定的减蛋综合征病毒 E05株中分段扩增六邻体蛋白基因，分别与克隆载体pMD18-T连接后，转化感受态细胞，对经PCR鉴定和酶切鉴定均为阳性的克隆进行测序，结果表明，E05株六邻体蛋白基因长度为2733 bp，共编码910个氨基酸。

The epithelia are properly digested by trypsin before processed nubble cultivation.

在贴壁之前用胰蛋白酶对兔角膜上皮进行适当的消化，然后进行组织块贴壁培养。

The star of the Thunder told The Oklahoman he felt insulted when Lakers Coach Phil Jackson publicly stated how often he got to the free-throw line this season, a hint Jackson obviously hoped would be digested by referees in the first round.

雷霆队的明星表示他感觉被侮辱了，菲尔公然发表的言论--关于他本赛季太容易站上罚球线的问题--深深刺伤了他。杰克逊只是希望裁判们在第一轮的比赛中注意一下。

An old wives' tale suggests that swallowed gum sits in your stomach for seven years before it can be digested.

传说有一位老夫人，被她吞下的口香糖在她的胃内呆了7年才被消化。

These food must be first processed by microoganism in rumen then in reticulum and Omasum, and digested in abomasums at last. Whereas ferment secreted only by flocks and herds can't digest all food completely which maybe result in waste. If microorganism is involved in this procedure, it would help to digest the food completely and make a fully absorption by intestine and stomach.

这些食物必须经过瘤胃微生物作用后，由蜂巢胃及重瓣胃作二次作用，再次入皱胃进行消化作用，但是，牛羊本身所制造的酵素并不足以分解所有的食物，而造成浪费，这时若有微生物来帮助食物的分解，让肠胃的吸收更加快速、完全。

These nutrients are digested in gastrointestinal tract and a orbed into the body to e ure the normal growth and development of body and physiological functio .

这些营养素在胃肠道消化吸收并进入人体，保证机体的正常生长发育和各种生理功能。

Methods Human pancreas tissue was digested with collagenase P using a semi-automated pancreas-digestion system followed by purification in a HCA-Ficoll continuous gradient using Cobe2991 cell separator.

方法使用自制的半自动人胰腺消化分离装置及胶原酶P进行人胰腺的消化分离，用COBE2991细胞分离机及HCA-Ficoll纯化人胰岛细胞。

Recombinant adenovirus particles were produced after 293 cells transfected with recombinant Ad genomic plasmid DNA digested with PacI, then they were further amplified in 293 cells.

纯化所得腺病毒滴度约为8×1012pfu/L，当MOI=100时，腺病毒感染HepG1细胞的效率＞75％，Western Blot证实在感染重组体腺病毒的细胞中有相应基因产物的表达。

In addition, the purified IgY was digested with pepsin and the fragment with specific antigen binding was produced.

另外，IgY经胃蛋白酶（pH4.0，37℃）酶解后，得到片断抗体Fab。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。