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METHODS: The calvarium bone of new born rats was digested with bacterial collagenase and cultured to obtain osteoblasts and SP with different concentrations was then added.

采用新生大鼠颅盖骨消化法培养成骨细胞，再加入不同浓度的SP，观察细胞生长数量，测定碱性磷酸酶活性及观察细胞周期变化。

METHODS: The calvarium bone of newborn rats was digested with bacterial collagenase and cultured to obtain osteoblasts and SP with different concentrations was then added. The proliferation and activity changes of the osteoblasts were identified by counting the cells, determining the ALP activity and observing the changes of cell cycle.

采用新生大鼠颅盖骨消化法培养成骨细胞，再加入不同浓度的SP，观察细胞生长数量，测定碱性磷酸酶活性及观察细胞周期变化。

METHODS: calvarium bone of newborn rats was digested with bacterial collagenase and cultured to obtain osteoblasts and SP with different concentrations was then added. The proliferation and activity changes of the osteoblasts were identified by counting the cells, determining the ALP activity and observing the changes of cell cycle.

采用新生大鼠颅盖骨消化法培养成骨细胞，再加入不同浓度的SP，观察细胞生长数量，测定碱性磷酸酶活性及观察细胞周期变化。

The calvarium bone of newborn rats was digested with bacterial collagenase and cultured to obtain osteoblasts and SP with different concentrations was then added. The proliferation and activity changes of the osteoblasts were identified by counting the cells, determining the ALP activity and observing the changes of cell cycle.

采用新生大鼠颅盖骨消化法培养成骨细胞，再加入不同浓度的SP，观察细胞生长数量，测定碱性磷酸酶活性及观察细胞周期变化。

Methods Germ tube test, chlamydospore test, CHROMagar Candida and API20 kit system were applied to separate non-Candida albicans strains from Candida albicans. Then PCR was used to amplify the internal transcribed spacer region of ribosomal DNA from 4 species of common Pathogenic non-Candida albicans (Candida glabrata, Candida parapsilosis, Candida krusei and Candida tropicalis), and the products were digested with the two restriction endonucleases (Msp Ⅰ and Hae Ⅲ) respectively. Results The four isolates consisted of 15 strains of Candida glabrata (7.50%), 7 strains of Candida parapsilosis (3.50%), 5 strains of Candida krusei (2.50%), 2 strains of Candida tropicalis (1.00%).

首先采用芽管试验、厚壁孢子试验、法国科玛嘉念珠菌显色培养基及API20CAUX酵母菌鉴定系统将分离自VVC患者阴道内的念珠菌菌株鉴定到种，然后采用真菌通用引物将4种常见非白念珠菌（包括光滑念珠菌、近平滑念珠菌、克柔念珠菌和热带念珠菌）进行PCR扩增，并选用MspⅠ和HaeⅢ两种内切酶对扩增产物进行酶切分析。

On the contrary, after a meal is a kind of soup detrimental to the health of eating soup will not only dilute the mixture a good chyme has been digested, but also dilute the stomach acid needed to digest food, affecting digestion.

相反，饭后喝汤是一种有损健康的吃法，汤不仅会稀释已被消化液混合好的食糜，还会冲淡食物消化所需要的胃酸，影响消化。

In feed add glucanase, xylanase, cellulose-based compound enzyme enzymes, anti-nutritional factors can degrade into small molecules fragments, the release of nutrients, reduce chyme viscosity, so that nutrition the material can be fully digested and absorbed to reduce the ileum bacteria substrate, inhibiting growth and reproduction of harmful micro-organisms to prevent diarrhea.

在饲料中添加以-葡聚糖酶、木聚糖酶、纤维素酶等为主的复合酶制剂，能够将抗营养因子降解成小分子片段，释放营养物质，降低食糜粘度，使营养物质能被充分消化吸收，减少回肠中细菌的底物，抑制有害微生物生长繁殖，防止腹泻。

Methods The suckling Balb/c mouse heart tissues were obtained and digested with trypsin and coagulase Ⅱ. The effects of digestion procedures on the primary cultivation of suckling Balb/c mouse myocardial cells had been compared.

取乳鼠心脏，采用胰酶和胶原酶Ⅱ消化方法分离乳鼠心肌细胞，比较消化次数和消化时间对原代乳鼠心肌细胞培养的影响。

The freezing slices of brain tissue were made, which different locations were collected from the brains of 12 catties. Then using the antiserum as the first antiboty and the monoclonal antibody 4C11 as the collator, the slices digested or undigested by protease K were dyed by ABC dyeing. The result was used to determined the location of PrP in cattle brain.

以12健康头黄牛脑组织为研究对象，采取脑组织的不同部位制成冰冻切片，以自制抗血清为一抗和单抗4C11为参照，应用ABC染色法对蛋白酶K处理的和未作处理的切片染色，检测牛脑组织中朊蛋白的分布。

RESULTS:（1）Three KDR-siRNA expression vectors were digested by Nde Ⅰ and Sma Ⅰ and produced two fragments of 713 bp, 5 480 bp and 2 403 bp, 3 790 bp, respectively, which were congruous with expectation.

KDR-小干扰RNA对A549细胞中KDRmRNA水平的影响：与阳性对照组、阴性对照组、空白对照组和正常对照组的A549细胞相比，KDR-siRNA1，2，3表达载体转染后的A549细胞KDR基因表达水平均明显受到抑制，抑制率分别为64%、81%和72%，其中以KDR-siRNA2抑制作用最为明显。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。