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Methods: After the sample being digested, because of ethylenediamine-hydrochloric acid, aluminium, chrome azurol S, the polyethylene glycol octyl phenyl ether together with hexadecylpyridine bromide form a steady blue quaternion system, whose absorbance and aluminium content is direct ratio within some consistence range.

样品经消化后，在乙二胺－盐酸缓冲介质存在下，铝与铬天青S和聚乙二醇辛基苯醚及溴代十六烷基吡啶形成稳定蓝色四元体系，在一定浓度范围内，其吸光度与铝含量成正比。

An improved method that facilitates the extraction of PCR-compatible faecal DNA from giant panda\'s faeces is described. The method involves a novel preprocessing step in DNA extraction. The faeces is washed two or three times with precooled acetone, which removes numerous potential PCR inhibitors, and then digested with proteinase K. The DNA is purified with phenol/chloroform.

在粪便DNA的提取过程中采用一个新的预处理方法，将粪便用预冷的丙酮洗2～3次，除去粪便中含有的大量PCR抑制物，然后蛋白酶K裂解、酚-氯仿抽提，能提取到纯度很高的DNA供PCR扩增。

It is rich in nutrient and is easily digested so you can't be fed with each and every single one of the various kinds. The porridges here in this restaurant are from all over China, be it porridges made on fast fire from the South, or the porridge made from mixed crops from the North. We also provide you with different types of porridges according to the change in seasons.

这里的粥集合了全国各地的口味，既有南方明火走的生滚粥类，也有北方的杂粮粥，种类多达20余种，并且根据时令的不同不断推出季节性的食品，例如在冬天喝一碗新鲜的山楂莲子粥，那简直是让人想起来就咽口水的事情。

The EcoR digested of random recombinant plastids showed that each had been inserted by cDNA sequences.

随机挑取酶切质粒的电泳分析表明，载体中均有插入片段。

4H was the best time of bovine oocyte activationin the 6-DAMP, and the cleavage rates and blastula rates could reach 85.1% and 26.8%. 4. IVF-100 was used in vitro fertilization, because IVF-100 was not needed to prepare usually,store conveniently, and gain a higher cleavage rates. After the oocytes have matured for 20h, theywere digested by permease and treated with suctionpipe.

由于IVF-100无需经常配制、保存方便且可以获得较高的卵裂率，所以本实验采用IVF-100为受精液，卵母细胞成熟20小时后采用透酶+口吸管消化的方法处理，获得了较好的受精效果，卵裂率可达(76.8％)。

The genomic DNA of Streptomyces diastaticus SM33 was extracted and partially digested by Hind III. The 40~60 kb high molecular weight DNA was extracted and ligated to pIndigoBAC536. The ligation mixture was transformed into EPI300 competent cells. A total of 5184 colonies were obtained by white-blue screening.

提取淀粉酶链霉菌SM33基因组DNA，用Hind III部分酶解后回收40~60 kb大小的高分子量DNA，与质粒载体pIndigoBAC536连接，电击转化EPI300感受态细胞，经蓝白斑筛选共挑取了5184个白色克隆。

The genomic DNA of Streptomyces diastaticus SM33 was extracted and partially digested by Hind Ⅲ. The 40~60 kb high molecular weight DNA was extracted and ligated to pIndigoBAC536.The ligation mixture was transformed into EPI300 competent cells. A total of 5 184 colonies were obtained by white-blue screening.

提取淀粉酶链霉菌SM33基因组DNA，用HindIII部分酶解后回收40kb~60kb大小的高分子量DNA，与质粒载体pIndigoBAC536连接，电击转化EPI300感受态细胞，经蓝白斑筛选共挑取了5184个白色克隆。

Sanitation index of treatments met the national standard of China Sanitary standard for the non-hazardous treatment of night soil (GB7959-87), except the colititer of composts with wheat-straw in winter and the compost added anaerobically digested effluent in summer.

除冬季的麦秆堆肥和夏季添加猪粪水厌氧消化液堆肥的粪大肠菌值超标外，其它卫生指标均达到了《粪便无害化卫生标准》(GB7959-87)。

Null mutant Δmnh6 produced appeared offwhite hyphae, showed reduction in growth, conidiation and appressorium formation, and also showed reduction in appressorium turgor pressure. And the hyphae were more readily wettable by a solution containing SDS and EDTA and more easily digested by fungal cell-wall-digesting enzymes. The appressoria produced by Δmnh6 mutants showed diminished host penetration and the infectious hyphae of Δmnh6 mutants showed reduced in planta growth. Targeted deletion of mnh6 resulted in the greatly reduced virulence capacity to barley and rice CO39 in infection assays.

突变子Δmnh6的菌落形态不同于野生型GUY11，菌落呈灰白色，菌丝稀疏，生长速度变慢，产孢菌丝细胞壁的溶剂疏水性消失，菌丝细胞壁容易被细胞壁降解酶降解，产孢减少，孢子形态变得细长，孢子的附着胞形成率降低，附着胞的膨压降低，附着胞对寄主植物表面的穿透能力减弱，侵入后生长速度减慢，喷雾接种后对大麦和水稻CO39的致病性消失。mnh6基因重新引入Δmnh6后，突变子的表型恢复。

The hFCECs were cultured by sticking tissues piece method that the cornea were divided into the limbus and central tissue piece and digestive method,respectively.For digestive method,the digestive juice of 5mg/ml DispaseⅡ+0.25%trypsin was chosed.D/F12 with 10%fetal bovine serum and 100 U/ml penicillin and streptomycin were used in this study,the culture condition was 37℃and 5%CO2,and culture medium changed every three days2、Passage of hFCECsAfter more than 80%confluenced,cells from corneal limbus hFCECs were digested for 5-15min with different concentrations of trypsin/EDTA at 37℃,and then passaged at a ratio of 1:2.The cells were subcultured on empty plates,mouse 3T3 fibroblast feeder layer,fetal corneal stromal cell feeder l ayer or HTK feeder layer respectively.And D/F12, mouse 3T3 fibroblast conditioned medium,fetal corneal stromal cells conditioned medium or HTK conditioned medium with 10%FBS and 100 U/ml penicillin and streptomycin were used respectively as the culture medium.And the culture medium were changed every three days as well.

方法一、人胎儿角膜上皮原代和传代培养1、原代培养严格无菌操作获取人胎儿角膜片，采用组织块贴壁法、酶消化法原代培养人胎儿角膜上皮细胞。2、传代培养角膜缘部的细胞生长融合达80%以上，不同稀释浓度的胰酶/EDTA消化液消化传代分别接种于空板、小鼠3T3成纤维细胞饲养层、胎儿角膜基质细胞饲养层及HTK饲养层，培养液分别采用D/F12、小鼠3T3成纤维细胞条件培养液、胎儿角膜基质细胞条件培养液、HTK条件培养液。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。