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Two molecular forms of Xenopus hatching enzyme, 60kD and 40kD molecules, was obtained during preparation and purification. Both of them were verified as the hatching enzyme molecules, using anti GST UVS.2 antibody as the probe. 60kD molecule was digested or autodigested easily into 40kD molecule during purification. It was indicated that 40kD molecule was probably derived from 60kD molecule with its two CUB repeats lost, and the two CUB repeats may play an important role in recognizing and/or modifying of the molecular structure of vitelline envelope.

在分离纯化非洲爪蟾孵化酶时，得到了60kD和40kD两种分子，用孵化酶的特异性抗GST-UVS.2抗体进行Western杂交的结果证明二者均为孵化酶分子。60kD分子很不稳定，在纯化过程中极易降解，40kD分子可能是由60kD分子经过降解或自身降解丢失了两个CUB重复区而形成的，而CUB重复区很可能在对受精膜分子结构的修饰或改造中具有重要作用。

And then, the pBI101.2 wirelike vector and the promoter fragment of 886bp were acquired. Becase the promoter was modified by the HindⅢand BamH I, the acquired target genes fragment, digested by the restriction endonuclease, was matched to the wirelike vector.

由于在启动子两端有人工设计合成的HindⅢ和BamHⅠ酶切位点，经上述双酶切消化后，回收目的基因片段与线状载体的粘性接头恰好匹配。

Those carbohydrates not digested to monosaccharides by human enzymes in the small intestine may be metabolized by microorganisms in the large intestine, producing substances that are absorbed and catabolized for energy.

那些不能被人体小肠中的酶消化至单糖的会被大肠中微生物代谢，产生能被吸收和用于能量代谢的物质。

Those carbohydrates not digested to monosaccharides by human enzymes in the small intestine may be metabolized by microorganisms in the large intestine, producing substances that are absorbed and catabolized for energy.

无知的人开导更无知的人。7。那些不能被人体小肠中的酶消化至单糖的会被大肠中微生物代谢，产生能被吸收和用于能量代谢的物质。

Restriction endonuclease digested genomic DNA was circularized by self-ligation, and complete gene of α subunit and β subunit was obtained by inverse PCR.

以环化的限制性内切酶消化的基因组片段为模板，用反向PCR的方法扩增、克隆并测定了α亚基和β亚基的完整基因序列。

At the same time, the purified soil DNA was partially digested by enzyme Sau3AI, and the 2-6kb and 6-9kb fragments were recovered by dialyzing respectively.

纯化后的土壤DNA用限制性内切酶Sau3AI随机切割，以透析袋分别回收2-6kb和6-9kb的酶切片段，然后用T_4连接酶与上述脱磷载体连接。

The crude DNA pellets were recovered by dialyzing and then could be used for molecular operation such as amplification of 16S rRNA gene and digestion of restriction enzymes.The plasmid pUC19 was prepared and digested with BamHI.

粗提DNA用半透膜透析回收纯化，纯化后的DNA可以进行16sRNA基因PCR扩增，酶切等分子生物学操作，具有较高的质量。

They are suckled for two months, but at the end of that time they are able to eat half-digested flesh disgorged for them by their dam or even their sire.

他们是哺乳两个月，但在去年底，当时他们都能够吃半消化肉disgorged为他们自己的大坝或什至他们的妻妾。

Some books are to be tasted,others to be swallowed,and some few to be c hewed and digested.

一些书可以品味，另外一些书可以吞食，少数的一些可以咀嚼、消化。

Genomic DNA of maize was digested with restriction enzymes and hybridized with a mF2KP cDNA fragment.

应用不同的限制性内切酶消化玉米基因组DNA，以〓p标记的mF2KP cDNA片段作为探针进行Southern杂交分析。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。