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Digested by endonuclease, the linear plasmid was injected into the copulatory pouch of the female moth and the sperm reservoir of the male moth. Let them mate with the normal moth and oviposit.

将打靶质粒酶切线性化后，分别注入到雌蛾交配囊和雄蛾贮精囊，与正常蛾交配产卵，分析外源基因在当代的情况。

When the embryo reached the prehatching stage, the hatching enzyme granules were secreted into the perivitelline space from the top of HGCs. Secretory granules were spherical and 0.5-1.0 μm in diameter. The hatching enzyme was dissolved in the perivitelline fluid, where it gained access to the egg envelope and digested the inner layer of the egg envelope.

至孵化前期时，孵化酶颗粒自HGCs顶部的开口分泌出来，分泌颗粒呈圆球形，直径为0.5-1.0 μm，有的以单体的形式存在，有的粘结成团。

A pouchlike enlargement of a bird's gullet in which food is partially digested or stored for regurgitation to nestlings.

嗉囊鸟类食管部位的囊状的扩大部分，用来对食物进行部分消化或贮存食物进行反刍来喂食雏鸟

A pouchlike enlargement of a bird's gullet in which food is partially digested or s to red for regurgitation to nestlings.

&鸟类食管部位的囊状的扩大部分，用来对食物进行部分消化或贮存食物进行反刍来喂食雏鸟。&

A pouchlike enlargement of a bird's gullet in which food is partially digested or stored for regurgitation to nestl in gs.

&鸟类食管部位的囊状的扩大部分，用来对食物进行部分消化或贮存食物进行反刍来喂食雏鸟。&

To study the biologicaleffect of pulsed electromagnetic fieldson the proliferation of Immunomagneticseparated human precartilaginous stem cellsin vitro.To establish immortalizedhuman precartilaginous stem cellsstrain and provided stable cell resource to studythe molecular mechanism of gene targeting on differentiation of PSCs.①The cells from the aborted fetus\'s metaphysis were digested by collagenase,the PSCs were isolated by magnetic-activated cell sorting,then were subculturedand amplified.

探讨体外分离、培养人前软骨干细胞的可行性，分析其表型特点；研究脉冲电磁场对免疫磁性细胞分选的人前软骨干细胞增殖的生物学影响；构建猿肾病毒40大T抗原基因(SV40Tag)介导的永生化人前软骨干细胞株，为下一步基因打靶研究其分化分子机制提供稳定的细胞来源。

The mutant △RSD-9 K141D was subcloned into the pGEX-6p-1 expression vector and the △RSD-9 protein was purified by GST affinity chromatography and digested by Prescission Protease to cut the GST domain. Next, to further verify the intracellular interaction of RSD-9/△RSD-9 and rtSH3p13 proteins, laser confocalization study and co-immunoprecipitation method were used.

随后，我们又运用蛋白质在细胞内的荧光共定位实验和免疫共沉淀实验，在细胞内相互作用水平上，证明了RSD-9蛋白/△RSD-9蛋白都与rtSH3p13蛋白在胞内相互作用。

To investigate the molecular diversity of the avian Salmonella, 12 S. typhimurium strains and 12 S. pullorum strains were identified by automatic microbiological assay and their genomic DNA were digested by restriction endonuclease Xba Ⅰ and typed by Pulsed-Field Gel Electrophoresis.

为应用脉冲场凝胶电泳从分子水平上对禽源沙门氏菌之间的差别进行分析和研究，本研究采用自动微生物鉴定仪对12株疑似鼠伤寒沙门氏菌和15株疑似鸡白痢沙门氏菌进行了鉴定。

Methods:Cardiac muscle of twoday neonate rattus was digested repeatedly by 0.0625% trypsin.The cardiocytes were collected in DMEM medium,which contains 10% fetal bovine serum.Myocardial cells were isolated with the technique of differential anchoring velocity with 90 min and were pured by Brdu.The cardiocytes were placed into CO2 incubator to incubate seven days after their purification.

应用0.0625%的胰蛋白酶重复消化出生第2 d乳鼠的心肌组织多次，收集的细胞用含10%胎牛血清的DMEM培养基中和，用差速贴壁分离法分离，在以溴脱氧尿嘧啶纯化心肌细胞后置CO2培养箱孵育7 d。

The oil yield rate of sewage sludge is higher th an that of putrescible garbage,the oil yield rate of activated sewage sludge i s the highest,lacquer sludge and digested sludge in successi...

低温热解制油技术的操作条件对产油率也有不同程度的影响，产油率随着热解温度的升高而增加，随着停留时间的增加而减少。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。