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Methods After the sample of Fenneropenaeus chinensis, Litopenaeus vannamei, Liza haematocheila, Acanthogobius hasta, Mactra veneriformis, Aloidis laevis, Ostrea denselamellosa and Fenneropenaeus chinensis was digested with concentrated hydrochloric acid, the lead content in marine products was measured by fluorimetric method and the results were compared with dithizone spectrophotometry.

采用湿式消解法对中国对虾、南美白对虾、梭鱼、矛尾刺虾虎鱼、四角蛤蜊、光滑兰蛤、密鳞牡蛎、中国对虾样品进行处理，采用浓盐酸作溶剂，荧光分光光度法直接检测铅含量，并与双硫腙光度法的测定结果进行比较。

In addition, our works also indicate that MC-ICPMS technique shows a good potential on Re-Os isotopic measurement. 2. A new method for the determination of low Re-Os abundances and ~(187)Os/~(188)Os in a natural rock sample by MC-ICP-MS has been established in our laboratory. Rock samples were digested in inverse aqua regia in sealed Carius tube for 24 hours at 230°C, then Os was separated from the matrix by CCl_4 solvent extraction, and further purification by microdistillation.

建立了Re-Os同位素样品的化学分离和纯化方法以及MC-ICPMS质谱测定方法：岩石样品的Re和Os化学分离方法是采用Carius管溶样法，结合四氯化碳萃取以及氢溴酸反萃取的方法分离出Os，最后是通过微蒸馏的方法纯化Os；利用阴离子交换树脂的方法分离纯化Re。

Furthermore, influences of different phosphate salts at various concentrations on the ionization efficiency of phosphopeptide were investigated systematically, finding that the signal intensity for phosphopeptide 48FQEEQQQTEDELQDK63 digested from β-casein were 5 to 8 times increased in an optimized condition with 10 mmol/L ammonium monobasic phosphate or 3 to 4 times increased with 10 mmol/L ammonium dibasic phosphate as additive to matrix 2,5-dihydroxybenzoic acid, respectively.

考察了两种适合于磷酸化肽离子化的基质类型2，5-二羟基苯甲酸和2，4，6-三羟基苯乙酮。用2，5-二羟基苯甲酸作为基质时，当加入10 mM 磷酸氢二铵时，磷酸化蛋白质β-casein的磷酸肽 48FQEEQQQTEDELQDK63的离子化效率可以增强5-8倍，当加入10 mM磷酸二氢胺时，磷酸肽的离子化效率可以增强3-4倍。

METHODS: Rat bilateral olfactory bulbs and olfactory mucosa at 1/3 nasal septum were obtained, sliced, digested in trypsin, and made into monoplast suspension. At 1×109/L, cells were incubated in uncoated 25 cm2 culture flask. At 18-20 hours, cell suspension was moved into another uncoated 25 cm2 culture flask (the first differential adhesion). At 24 hours, cell suspension was moved into a poly-D-lysine-coated 25 cm2 culture flask or poly-D-lysine-coated 6-well culture plate (the second differential adhesion). At 48 hours, parenchyma cells were removed after a half of medium was changed.

完整取大鼠双侧嗅球及剪取鼻中隔后1/3嗅黏膜，剪碎后胰酶消化，制成单细胞悬液，按1×109 L-1密度接种于未包被的25 cm2玻璃培养瓶中，18~20 h后将细胞悬液转移至另一未包被的25 cm 2玻璃培养瓶中（第1次差速贴壁），24 h后再将细胞悬液转移至经多聚右旋赖氨酸包被的25 cm2塑料培养瓶或经多聚右旋赖氨酸包被的6孔培养板中（第2次差速贴壁），接种培养48 h后半量换液以去除杂细胞。

METHODS: Olfactory bulb was sterilely collected from rats, digested, and centrifuged. After removal of the supernatant, the olfactory bulb was resuspended with DMEM/F-12 medium containing 0.2 volume fraction of fetal bovine serum. The diluted monoplast suspension was equally assigned into 3 samples, and separately used for simple modified differential adherence, simple serum-free conditioned medium, and modified differential adherence + serum-free conditioned medium.

①无菌条件取大鼠嗅球组织，消化、离心去除上清液后，用含体积分数为0.2胎牛血清的DMEM/F-12培养基重悬，稀释的单细胞混悬液均分成3份，分别用于单纯改良差速贴壁、单纯无血清条件培养液纯化和改良差速贴壁+无血清条件培养液纯化的实验。

Digested by restriction endonuclease,and cloned HPC2 into mutic...

人HPC2基因的原核及真核表达载体的构建为深入研究HPC2基因与宫颈癌的发生奠定了基础。

The rest of proteins, peptides and amino acids are reabsorbed by podocytes and labyrinth cells, then become formed bodies and secreted into urinary space again and flow with the urine. These nutritions are digested in formed bodies and then reabsorbed and utilized by cells of nephridial canal.

该滤液中的葡萄糖和部分蛋白质、多肽、氨基酸由足细胞重吸收并消化利用，剩余部分蛋白质、多肽和氨基酸由足细胞和迷路细胞重吸收形成形成小体后再进入尿隙随尿液流动，并在形成小体中被消化，最后被原肾管细胞吸收利用。

The EM sludge with digested sludge as seed could enhance the removal efficiency of ammonia and nitrogen in wastewater, because the rate of nitrify reaction was much more quickly, and the nitrify time was less than 3hr half of the routine active sludge.

而EM污泥脱氮效果较差。(9)两种污泥中EM引种污泥对磷的降解效果较好，与常规活性污泥对照，降解率增幅达5%~10%；而EM污泥对磷的去除率低，低于常规污泥反应器。

After being digested with EcoR I and Xho I, they were inserted to the vectors pGEX-4T-l orientally, and then transformed into E.coli BL21(DE3) cells. The monoclone were selected and identified by means of restriction analysis, PCR and sequencing. As the result, the two recombinant plasmids with the different DNA fragements of the PrP gene were constructed and designated as pMY01[Ov rPrP(23-244)] and pMY02[Ov rPrP(91 -244)], respectively.

挑选单个菌落，提取重组质粒，经酶切、PCR扩增后琼脂糖凝胶电泳分析和测序鉴定，成功地构建了2种绵羊重组朊蛋白的表达质粒pMY01[OvrPrP(23～244)]和pMYO2[Ov rPrP(91～244)]。

In this study, the structure sequences of Shiga toxin 2B(stx2B) and Shiga toxin 1B(stx1B) were respectively amplified from EHEC O157:H7 by PCR. stx2B-L-stx1B fusion gene was constructed by a 12 bp linker ligating these two fragments. After digested with restriction endonuclease Nco Ⅰ and EcoR Ⅰ, the fusion gene was orientally inserted into expression vector pET28a.

利用PCR方法，从肠出血性大肠杆菌O157：H7的基因组DNA中，分别扩增出Stx1B和Stx2B亚基的结构基因片段，然后再通过PCR将这2个片段连接起来，并定向克隆到表达质粒pET28a的NcoⅠ和EcoRⅠ位点之间，构建了重组表达质粒pMB1。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。