查询词典 digested tankage

While the configurations of Figure No's 23, 24 and 25 show Bio- Module units in steel tankage, they can also be used for Bio-Module shafts in concrete tankage.

图23，24，25展示了钢池中的生物模块设备，这些设备也可以用在水泥池中作为生物模块的轴。

Then pMD-P80 is digested by the enzymes Xho I and Apa I, separated and linked to the linear plasmid pEGPF-C1 after digested by Xho I and Apa I. So the recombined expression plasmid pGFP-P80 is achieved. Then it is sequenced, PCR and digested by the restriction enzymes Xho I and Apa I. The results give that pGFP-P80 is achieved successfully and the insert sites, direction and reading frame are all right. It builds the basis for expressing, purifying, gaining p80 protein from mammiferous cells.

将pMD-P80 分别经Xho I 和Apa I 双酶切和回收，然后与经过Xho I 和Apa I 酶解的真核表达载体pEGPF-C1 连接、转化，获得重组质粒，经PCR，Xho I 和Apa I 限制性酶切和序列测定，鉴定为真核表达质粒pEGFP-P80，并且目的基因的插入位置、方向和读码框完全正确，为在哺乳动物细胞中表达并分离纯化p80 蛋白奠定了基础。

Can there provide animal egg white feed digested tankage feather powder meat bone meal pet egg white feed?

那里能提供动物蛋白饲料肉粉羽毛粉肉骨粉宠物蛋白饲料？

Calvaria was obtained and scissored into pieces on the super clean blench through sterile method after fetal Kunming mouse within 24 hours were killed by cervical dislocation in the experiment. The bone pieces were digested by 0.25% trypsin for 30 min at 37℃ at first, then digested by 0.2% collagenase at 37℃ in constant temperature oscillator for 60 min, and rinsed repeatedly to disaggregate more single-cells.

试验采用24h内昆明乳鼠，拉颈处死，无菌取出头盖骨，在超净工作台内将其剪碎，先用0.25%胰蛋白酶37℃消化30min，然后用0.2%胶原酶37℃振荡消化60min，反复吹打骨片分离更多细胞。

To investigate the mechanisms of biohydrogen production by anerobic fermentation of solid organic waste, gas chromatography is used to analysis the biogas and volatile fatty acid in anaerobic bio-reactor. Firstly, the ability of hydrogen production by digested sludge from the West Lake, Enteobacter aerogences, digested sludge from sewage farm, the fluid of methane pool and dejecta have been studied. The rule of the ability of hydrogen production by different bacteria under different control condition. Simultaneity, the importance of the synergistic effect on hydrogen production has been proved.

本文首先选用西湖底的厌氧活性污泥、产气肠杆菌、污水处理厂的淤泥、沼气池发酵液以及猪粪等不同菌群对废弃食物——马铃薯进行厌氧发酵产氢特性实验研究，得到马铃薯在各不同菌群及工况下的发酵产氢能力，同时发现产氢菌间的协同作用很重要，在控制好发酵条件的情况下，产氢菌群发酵通常会好于单一产氢菌的发酵。

Articular chondrocytes from the knee joints of the A group were separated respectively to fragments, and then digested by enzymes, both the chondrocytes and the uncomplite digested tiny chondral tissues have been cultured in vitro for about 2 weeks.

分别取A组9只兔子的双侧膝关节股骨滑车及部分髁部的软骨，在体外进行分离和消化，未完全消化的软骨组织加软骨细胞在培养瓶内一同培养2周后配制成浓度为1.6×10~8/ml的软骨细胞悬液，等分装于两个EP管中。

The Charon library DNA was then digested with restriction enzymes BamH Ⅰ and Hind Ⅲ, and DNA fragments of length of about 0.5-1. 5kb was subcloned into plasmid pUC 18 which was also digested by BamH Ⅰ and Hind Ⅲ and dephosphorylated by alkaline phosphatese before being ligated with Charon fragments.

抽提λCharon文库总DNA，用BamHⅠ和HindⅢ完全酶切电泳，用低熔点琼脂糖回收长约0.5-1.5kb的DNA片段；连接到pUC18上，pUC DNA先用BamHⅠ和HindⅢ酶切，后用碱性磷酸酯酶脱磷。

Torenia fournieri genomic DNA was digested with DraI、EcoRV、PvuII、SmaI respectively. A special adaptor was ligated to the ends of the digested DNA fragments as a template for adaptor PCR. With the adaptor and TfPLC1 gene-specific primers, bands of 798bp and 813bp upstream of TfPLC1 were obtained successively.

应用衔接头PCR技术，以蓝猪耳全基因组DNA分别经DraI、EcoRV、PvuII、SmaI消化后与衔接头连接产物为模板，用衔接头引物和TfPLC1基因的特异引物经过多轮的巢式PCR，先后克隆到两个大小为798bp、813bp的TfPLC1基因上游序列；经测序、blastn比较分析和拼接得到一个蓝猪耳TfPLC1基因的启动子序列，共1432bp。

We biopsied 1-2 single blastomere from 6-8 cell cleavage stage intracytoplasmic sperm injection embryo, and using nested PCR amplified the high frequently mutation region G380R of FGFR3 gene of the single blastomere. The products of PCR were digested by restriction enzyme Bfm Ⅰ, then the digested products were detected by 10% PAGE to see whether the embryo inherted the mutation of the patient and to screen out normal embryo transfer.

活检经胞质内单精子注射(intracytoplasmic sperm injection，ICSI)授精的胚胎发育至6～8细胞期的1～2个单卵裂球，采用巢式PCR扩增单卵裂球的FGFR3基因的高发突变位点G380R区域，用限制性内切酶BfmⅠ消化巢式PCR的内扩增产物，再经10%聚丙烯酰胺凝胶电泳检测有无遗传患者的该种突变，从而筛选出正常胚胎移植。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

Hanna: That's over now, isn't it?

都结束了，对吗

You must be ill. You look so pale.

你一定是病了，你的脸色苍白。