查询词典 differentially

The improved PCR-based subtractive hybridization might be efficient for cloning of differentially expressed genes in target cells.

结论这种基于PCR技术的改良消减杂交方法对于差异表达基因的克隆是十分有效的。

The Student's t statistic is used in one stage log-ratio models to identify differentially expressed genes.

本研究最后也透过一阶段对数比模型和gene-by-gene模型来讨论决定重复数的问题。

Results (1) The forward and reverse subtracted cDNA libraries of different metastastic potential large cell lung cancer cell lines were successfully constructed;(2) With blue-white colony and dot blot, 307 positive clones in the forward subtracted library and 78 positive clones in the reverse subtracted library were obtained;(3) 55 clones were successfully sequenced in the forward subtracted library. Homolog analysis confirmed 23 differential expression segments, which were similar to the human genes already known, including NME2, NPM1, MT 2A, HSPE1, TAFIA, EPRS, PX19 and EIF3S9 et al;(4) 31 clones were successfully sequenced in the reverse subtracted library. Homolog analysis confirmed 16 differentially expressed segments. 15 of them were similar to the human genes already known, including ANXA2, TUBB, PKN2, GNAS, EEF1A1, SSR2 and RPLPO et al. Only one segment had partial homology to known human genes. This segment was supposed to be the new EST segments which might not have been cloned.

结果(1)成功构建人大细胞肺癌高低转移株差异表达基因正向消减cDNA文库和反向消减cDNA文库；(2)经蓝白菌落筛选和斑点杂交，正向消减文库获得307个阳性克隆，反向消减文库获得78个阳性克隆；(3)正向消减文库挑选55个克隆成功测序，经同源性分析最终确定差异表达基因片段23个与已知人类全长基因具有很高相似性(95％～100％)，这些基因包括NME2、NPM1、MT2A、HSPE1、TAFlA、EPRS、PX19和EIF3S9等；(4)反向消减文库挑选31个克隆成功测序，经同源性检索和比对最终确定差异表达基因片段16个，其中15个与人类全长基因具有很高相似性(95％～100％)，包括ANXA2、TUBB、PKN2、GNAS、EEF1A1、SSR2和RPLPO等基因。1个片段和己知人类基因仅有部分相似性，表明它可能是未被克隆的人类基因EST片段。

4When the cover settles differentially, some part of it will be subjected to tension and will undergo tensile strain.

4垃圾填埋场的最终覆盖系统过大的差异沉降会导致土体开裂和土工膜断裂。

Objective To screen gene fragment differentially expressed in testicle in winter and summer.

目的 筛选出数个在冬夏季节变化中睾丸差异表达的基因片段。

Then Dot blot assay was used to select for and amplify differentially expressed cDNAs. Total 22 differential by displayed fragments were cloned and sequenced.

在SSH-NSC富集差异基因的基础上，我们采用了斑点杂交的方法对2个文库进行了差异基因的进一步筛选，共获得22个差异片段，克隆并测序。

This would indicate that each allele could be differentially regulated so that even one-third genome can be expressed in triploid individuals.

研究表明每个基因有其独特的调控机制，因而在杂种三倍体内三分之一的基因组亦能够表达产物。

Using semi-quantitative RT-PCR, the differential expression profiles of eleven selected genes were confirmed in the ovaries of triploid and diploid. These genes fell in gene categories with a wide range of functions. The results indicated that triploidy affects the dynamic gene regulatory network in triploid ovary. This study established a firm basis for future investigation on characterization of crucial molecular events for normal ovarian development in shrimp.To further dissect exact gene functions for gonad development of shrimp, three differentially expressed genes between diploid and triploid ovary, PCNA (proliferating cell nuclear antigen), CAS/CSE1 (cellular apoptosis susceptibility protein/chromosome segregation 1) and SSRF (spermatogonial stem-cell renewal factor) were characterized on certain aspects.

利用抑制性消减杂交技术，建立了对虾二倍体和三倍体卵巢间的2个消减文库；在正向消减文库(以三倍体卵巢作为实验组，二倍体卵巢作为驱动组)中，鉴定到54个基因；在反向消减文库(以二倍体卵巢为实验组，三倍体卵巢为驱动组)中，鉴定到16个基因；选取11个差异表达的基因，利用半定量RT-PCR的方法对其在二倍体和三倍体卵巢间的表达进行了检测，均能很好地与消减结果相吻合；这些差异基因编码多种功能的蛋白，分析表明染色体的三倍化使三倍体卵巢中的基因调控网络受到了影响；为深入揭示维持卵巢正常发育的关键分子调控事件奠定了基础。

The molecular response in the amoeboid and trophozoite stages of T .vaginalis was elucidated by using a systemic comparative transcriptomics and proteomics approach. We identified 49 differentially expressed proteins by 2-DGE and MALDI-TOF MS.

我们首先建立了阴道鞭毛虫滋养体的标准二维电泳蛋白图谱，再利用比较二维电泳结合质谱的方法找出了49个表现量有差异的蛋白。

Methods 8 conservative methods including Bonferroni adjustment and 7 others, two sample t test, Wilcoxon approach and SAM approach, all together 11 methods are used to analyze the simulated data. FDR and the number of detected differentially expressed genes are employed to evaluate these methods.

使用Bonferroni修正法等8种保守的方法以及两样本t检验、Wilcoxon非参数法、SAM共11种方法对模拟的芯片数据进行处理，以FDR和筛检的差异表达基因个数为指标考察其筛选效果。

We got alerted a couple of times while we were down south that HETs were on the way to bring us back up north because things were going to go hot again, but it was just rumors.

南下的途中我们不只一次得到警告说重型装备运输车将拉着我们重新北上，因为局势正在变得重新紧张起来，但这只是谣传。

It's the one where they find the ghost in the salt mine.

这一集是演他们在盐矿找到鬼