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Methods The differentially expressed genes between liver tumor and non-tumor tissue were cloned by using suppression subtractive hybridization. Differentially expressed genes were sequenced and analized for homology in Gene Bank. In situ hybridization was performed to identify special expression of differentially expressed genes in livre tumor and non-tumor tissues.

方法通过抑制消减杂交法克隆土拨鼠HBV引发的肝癌组织和非癌组织中差异表达的基因；对差异表达基因进行DNA序列测定后于Gene Bank内分析其同源性；应用原位杂交确定差异基因在肝癌组织和非癌组织中的特异性表达。

The number of differentially expressed genes was 71 at 24h, and 62 at 72h. The metabolisic gene class was the majority among all the differentially expressed genes, and the next was transportational、differentiation and adhesional gene class by turns.1.2. RT-PCR verification of the known HIF-1 targeted genes and proliferation related genesIn the 131 differentially expressed genes, we found 7 known HIF-1 targed genes (ldha、aldoa、eno1、gapdh、tpi1、pgk1、bnip3) and 5 genes (dnaja2、ccnb1、tgfβ2、ccng1、rgs7) which were related to proliferation and cell cycle.

对差异表达的基因进行基因功能分类发现：代谢类基因最多，其次是运输类基因、细胞分化及黏附类基因。1.2 RT-PCR验证HIF-1靶基因和增殖相关的基因由于我们的前期实验证明了低氧促NSC增殖是有HIF-1参与的，我们对131个差异表达基因中，7个已知的HIF-1靶基因(ldha、aldoa、eno1、gapdh、tpi1、pgk1、bnip3)和5个与细胞周期和增殖相关的基因(dnaja2、ccnb1、tgfβ2、ccng1、rgs7)做RT-PCR验证。

Expression patterns of genes differentially expressed in starch metabolism and cell cycle during endosperm development7 DAP kernals, 15 DAP endosperms and 25 DAP endosperms of B73, ae/wx and sh1 were used to study the expression patterns of 18 differentially expressed genes in sugar metabolism and 9 differentially expressed genes in cell cycle. We found that the expression patterns of Pul, SBEⅡa, UGPase, SUS3, SSⅡa in sh1 and Sh2-1, SUS3, SSⅡa in ae/wx were altered, revealing that these genes may interact with each other.

碳水化合物代谢基因和细胞周期基因随胚乳发育的变化以B73、ae/wx、sh1授粉后7天种子、15天和25天胚乳为材料，分析18个糖代谢差异基因和9个细胞周期调节相关的差异基因的表达模式，研究发现sh1突变体中Pul、SBEⅡa、UGPase、SUS3和SSⅡa基因表达模式发生改变，ae/wx突变体中Sh2-1、SUS3和SSⅡa基因表达模式发生改变，揭示了突变体对这些基因的巨大影响，反映了它们可能存在相互作用。

AIM: To detect the differentially expressed genes in the process of esophageal carcinoma induced by nitrosamine, and analyze the property of gene expression at different stages, especially the relationship between differentially expressed genes and DNA methylation.

摘要目的：通过基因芯片技术检测亚硝胺诱导食管癌变过程中，食管组织基因表达的差异，分析不同阶段基因表达的特点以及基因差异表达与DNA甲基化的相关性。

Methods The differentially expressed genes in the vascular tissue of diabetic gangrene foots were screened by the functional classific gene chip. Reverse transcription polymerase chain reaction detection was used to verify the differentially expressed 3 genes.

应用功能分类基因芯片筛选糖尿病坏疽血管组织中的差异表达基因；用反转录多聚酶链反应对3个差异表达基因进行验证。

To give consideration to both validity and reliability, six combinations are recommended when differentially expressed genes are less, RMA+fold-change, RMA+ SAM, RMA+ limma, PDNN+ fold-change, PDNN+SAM, and PDNN+limma. Three combinations are recommended when differentially expressed genes are more, dChip+ fold-change, dChip+SAM, and dChip+limma.

若同时注重信度与效度，我们推荐几种方法组合：当表现量差异基因个数少时，推荐RMA+fold-change、RMA+SAM、RMA+limma、PDNN+ fold-change、PDNN+SAM与PDNN+limma此六种组合；而当表现量差异基因个数多时，则推荐dChip+fold-change、dChip+SAM与dChip+limma此三种组合。

Of 8000 cDNA elements, custom cDNA array identified 47 as differentially expressed genes/ESTs with above 3-fold changes in the hippocampus 35 days after lesion, in which 25 were up-regulated and 22 down-regulated. Northern blot hybridization indicates that false ratio is high with the cDNA array experiment. Of 6234 rat CNS genes/ESTs, cDNA microarray identified 152 as differentially expressed cDNA clones with changes between 1.5-2.8 fold in the hippocampus 10 days following perforant path injury, 32 were up-regulated and 152 down-regulated.

Custom cDNA array 筛选的8000个cDNA 克隆中有47个在去神经30天海马显示差异表达且表达变化在3倍以上，其中25个表达上调，22个表达下调； Northern blot 实验表明cDNA array 的假阳性率是较高的。cDNA microarray在大鼠中枢神经系统表达的6234种基因和EST序列中，筛选到了152个基因和EST 序列在去神经10天海马差异表达，表达变化在1.5-2.8倍之间，其中表达上调的32个，下调的120个。

To elucidate the molecular mechanisms underlying the neural plasticity after CNS injury, the gene expression profile in the rat hippocampus following perforant path transections was explored by several methods including custom differential screening, custom cDNA array and cDNA microarray. Of 2300 cDNA clones from low-abundance rat brain cDNA bank, 6 were identified as differentially expressed genes/ESTs in the hippocampus 35 days after lesion, in which none was confirmed by Northern blot. Of 8000 cDNA elements, custom cDNA array identified 47 as differentially expressed genes/ESTs with above 3-fold changes in the hippocampus 35 days after lesion, in which 25 were up-regulated and 22 down-regulated.

中文题名去传入海马的差异表达基因筛选和Cystatin C表达副题名外文题名 Screening of the differentially expressed genes and cystatin C expression in the deafferented hippocampus 论文作者应国新导师周长福研究员学科专业神经生物学研究领域\研究方向学位级别博士学位授予单位中国科学院上海生命科学研究院学位授予日期2002 论文页码总数121页关键词海马星型胶质细胞 Cystatin C 基因表达去神经海马馆藏号BSLW /2003 /Q42 /27 为了阐明中枢神经系统损伤后神经可塑性的分子机制，本实验探索了多种方法，包括传统差异筛库、传统cDNA array和cDNA microarray，对穿通纤维切断后大鼠海马的基因表达图谱进行了研究。

According to the alignment of Blastn and Blastx, one differentially exp ressed fragment, A7, was 97% identical in nucleotides to the 26S ribosomal RNA gene of Photinia fraseri and 91% in amino acids to cytochrome P450 monooxygenase in maize, respectively. Another differentially exp ressed fragment, A16, was 85% in nucleotides and 93% in amino acids identical to alpha-expansin 2 in Triphysaria versicolor.

核酸和氨基酸比对分析中同源性最高的是片段A7和A16.A7的核苷酸序列同源于红叶石楠Photinia fraseri 26S核糖体RNA (97%)、蛋白同源于玉米的细胞色素P450单加氧酶(91%)， A16的核苷酸(85%)及蛋白(93%)同源于直果草属 Triphysaria versicolor 的α-expansin 2基因。

Extract total proteins from these two tissue samples respectively and then employ a SDS-PAGE method to identify the most differentially expressed protein between them and estimate the molecular weight and relative abundance ratio of the protein.

从这两个组织中分别提取总蛋白并定量，然后采用 SDS-PAGE 方法识别出在这两个组织中差异表达最为明显的蛋白质并且估计它的分子质量以及相应的丰度。

The use of the web to spread fears that flicking the switch on the LHC could create a Black Hole that could swallow up the Earth particularly concerned him, he said.

他说，使用互联网散布恐慌说启动LHC会生成一个可能吞噬地球的黑洞的做法让他感到忧虑。

Not a single word have I written down.

我一个字也没有写下来。