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After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

There is no such thing as a "ty pical" honeynet deployment.m A ho Female eynet n contain different kinds of honeypots, in different numbers,d set up ma Female y different ways.

之前，一个正确的蜜网设置需要许多时间来安装，配置和测试。蜜网网关必须整合好几个不同的工具来提供数据控制，数据捕获和自动化告警。

While Andalusite's color play has been compared to Alexandrite, this is technically incorrect as pleochroic (displaying different colors in different directions) gemstones like Andalusite feature all their colors at once, whereas Alexandrite only changes color in different light sources.

把红柱石的颜色变幻与亚历山大石相比，从专业角度来讲，这种比较并非科学，因为如红柱石一类的多向色性（不同方向呈现不同的颜色）宝石会在瞬间显现出她所有的颜色，而亚历山大石是靠不同的光源进行着颜色的变化。

Fixed number of year of the essence to praedial depreciation, depreciation and the different understanding that incomplete is worth and different processing technique, can reach different assessment result.

对不动产折旧的实质、折旧年限及残值的不同认识和不同处理方法，会得出不同的评估结果。

The method comprises A: separately configuring different common channel priority indication for different service data stream transmitted on same dedicated service channel; B: according to sequence from high to low of public channel priority indication for different data stream, priorly scheduling public channel high priority indication service data stream for transmitting at every moment; C: if service data stream of low common channel priority indication being not scheduled in a preset reference time period, then upgrading the service data stream common channel priority indication, then return to B.

分别为在同一专用业务信道上传输的不同业务数据流配置不同的公共信道优先级指示；B。在每一时刻，所述专用业务信道按照不同业务数据流的公共信道优先级指示由高至低的顺序，优先调度公共信道优先级指示高的业务数据流进行传输；并C。预设一参考时间段，若公共信道优先级指示低的业务数据流在所述参考时间段内总没有得到调度，则将该业务数据流的公共信道优先级指示升高，然后返回B。

In this paper, the different volume ratio additives'influences on the economy of different fuels as well as the characteristics of combustion and emission have been investigated employing the knowledge about combustion dynamics and chemical reaction dynamics on the base of the gasoline additives application actuality in engine. Dimethyl Carbonate, Diisopropyl Ether and Ditert-Butyl Peroxide are selected as gasoline additives to explore the potential and promotable additives by engine tests in different base fuels.

本文根据汽油添加剂的应用现状并结合内燃机燃烧学、化学反应动力学等知识，选择用碳酸二甲酯、二异丙醚、二叔丁基过氧化物作为汽油添加剂，通过不同基础燃油的发动机台架试验，研究不同添加体积比的添加剂对不同燃油经济性、燃烧特性、排放特性的影响，以探求潜在的可推广使用的汽油添加剂。

Strain LT6 exhibited significantly different antagonistic activities against different species of Pythium or different isolates of the same species.

腐霉菌株对由LT6产生的抗生素吩嗪-1-羧酸的敏感性存在变异，被测的54株腐霉，当PCA浓度为7.0μg/mL时，敏感的为28株，高抗的为15株。

The statistic analysis of the relationship between the operative amounts and the corrected amount of eye primary position of 177 operations on the horizontal recti showed a positive proportional relation. And the correction effect in degree for each mm of surgery is between 1. 2°～2. 2°, and for different types of strabismus and different surgical treatments, the correction effects for each mm of surgery are different.

对临床177例眼水平直肌手术的手术量与眼位矫正量的关系进行了统计分析，结果表明水平直肌的手术量与眼位矫正量有较好的正相关关系，大约每毫米手术量对应的手术矫正量在〓之间，不同类型的斜视、不同的手术，每毫米手术量对眼位的矫正量不同。

The algorithm is applied in the segmentation of Magnetic Resonance Imaging of human brain for the 3D reconstruction on dependent of different human tissue structures, in which the problem on wraps between different tissues and fuzzy edges between the different regions in medical volume data may better be settled, which can not be well done by the typical"hard"segmentation using binary logical representation.(3) An accelerated algorithm of volume rendering with X-ray perspective and Phong shading effect based on textures mapping and blending is presented for realizing 3D reconstruction of medical volume data by 2D texture mapping and blending, in which the accelerated algorithm of window transformation is utilized to computing voxels' gray scales and opacities to produce X-ray perspective images, and Phone illumination model is used to import world light sources and an unit spherical surface under the same shading environment as the actual volume rendering is employed to simulate the real reflectional light of per-voxel so that 3D reconstruction possesses illumination effect in the world. Furthermore, 3D matrix transformations of degree rotation and other operations of 3D object are studied, and a fast equivalent method of corresponding shading correction is brought forward to improve its interaction speed in volume rendering process.

该方法利用体数据的窗口变换加速算法将医学体数据转换为计算机显示值，可以得到具有X光透视效果三维图像；该方法提出的Phong明暗处理加速算法利用Phong光照模型引入外部光源，使用一单位球面体来仿真体数据的法线矢量集所产生的反射光强，以快速得到每一个体素的反射光强度，使物体的三维重建达到现实世界中人眼直接观察的逼真效果；此外，还研究了三维重建物体的三维旋转等矩阵变换，提出了一种Phong明暗修正的快速算法来保证了体绘制中三维几何变换的多视角观察的交互速度。

It is divided into four categories about the legal result of invalid marriage In many countries" modern legislation. The legal results for invalid marriage under different legislation are also different on the retrospectiveness and the result after being declared to be invalid. In the stipulation on the invalid marriage and the revocable marriage system, single system and dual-tract system also exist. Such kind of difference are the result of the different countries" politics, history,culture and legal tradition, etc.

对于无效婚姻的法律后果，现代世界各国的立法，大体上可归于四类不同的体例，各种不同的体例下对婚姻无效的法律后果，主要是对无效的后果及是否有追溯力的规定各不相同，在无效婚姻与可撤销婚姻的设置上，也存在着单一制与双轨制的区分，这种不同，主要取决于各国不同的政治、历史、文化和法律传统等因素。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。