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Three major clusters of numt sequences ( cyt b numt、 ATP8+ATP6 numt and ND4+ tRNA-His+ tRNA-Ser numt) were particularity amplified and sequenced from 10 species within Galliformes and A.galericalata. Comparison numts sequences with homologous mitochondrial partial genes get the different evolutionary characteristics and evolutionary speed compared to mtDNA, then examine the age of insertion of numts using the sequence divergence of numt from the mtDNA. Finally, joined Coturnix japonica、 Coturnix chinensis、 Lophura leucomelana、 Lophura swinhoii and A.albifrons mitochondrial sequences in GenBank, we study the importance function to phylogenetic research of mitochondrial genes and homologous numts.The evolutionary rate of Numts is lower than their authentic sequences of mtDNA, cyt b and ND4 gene evolve faster than homologous numts 2.5 times; ATP8+ATP6 gene evolve faster than numts 18.9 times in Galliformes. Numts show less codon position bias and transition bias under not phylogenetic pressure.

根据2004年3月GenBank登录的红原鸡核基因组草图与其线粒体基因组比对发现红原鸡核基因组中存在3段较长的线粒体假基因,利用PCR方法扩增鸡形目10种鸟类和雁形目中鸳鸯核基因组中3段numt序列:cyt b numt、ATP8及下游ATP6 numt和ND4+tRNA-His+tRNA-Ser numt,测序后分别比较线粒体基因与核同源numt序列的进化特点和进化速率,确定numt序列插入核基因组的时间,以及结合GenBank已登录的日本鹌鹑、蓝胸鹑、黑鹇、蓝鹇和1个雁形目物种——白鹅雁(A.albifrons)相应序列探讨两种同源序列对于鸟类系统进化发育重建的重要作用。

What\'s more, different intervals of UV have their own characters in influencing DNA: the damage to some bases by UVA may be more serious than that by UVB, and UVB did more severe damage to some deoxyribose than UVA; however, as far as the pyrimidine bases are concerned, the damage by UVB is by far more serious than that by UVA. These results may indicate that there are preferential damaging sites on DNA molecules caused by UVA and UVB irradiation. Moreover, with UVA or UVB irradiation for a long time, the photoreactivation of DNA occurred. The serious damage to deoxyribose and bases by UVB and UVC may confirm the conformation of cyclobutane pyrimidine dimers and (6-4) photoproducts.

而不同波段紫外辐射对DNA的损伤各具特点并且差异明显,UVA对一些碱基的损伤比UVB要严重,而UVB在对脱氧核糖和磷酸盐的损伤上又比UVA要严厉,但就嘧啶碱基来说,UVB对它的损伤远大于UVA,这似乎表明了不同的紫外波段对生物大分子DNA的损伤是有选择性的。

Results After different time of reperfusion, the score of ethological index in control group was higher than that in sham-group, that in breviscapine group was higher than sham group, but was lower than control group.

结果 沙土鼠脑缺血再灌注后1、2、4和7d,对照组相关行为学指标评分明显高于假手术组,而灯盏花素组沙土鼠行为学评分尽管高于假手术组,但明显低于对照组。

At last, ILIF in segment L3-4 and L4-5 is feasible. 2. There are enough operation space for ILIF in L3-4 and L4-5 with the root being retracted gently. And it is feasible to insert a couple transpedicle screws homolaterally. The intertransverse area was too narrow to perform ILIF on L5-S1.3. ILIF provide greater biomechanical stability than TLIF. while there was no significantly different stability between ILIF with homolateral or bilateral pedicle screw fixation. Adequate postoperative stability can be achieved by intertransverse lumbar interbody fusion with homolateral pedicle screw fixation.4. ILIF with transpedicle screws fixation homolaterally was a safe, convenient and effective operation, and less intraspinal complications were occurred of ILIF than TLIF.5. The fusion condition was satisfaction in ILIF group, which was better than TLIF group.

横突间入路腰椎椎体间融合术在L3-4、L4-5节段具有可行性。2、在L3-4、L4-5节段可提供足够的空间行ILIF手术,并可通过同一切口附加椎弓根钉固定,操作安全、简便。L5-S1节段横突间区域较小,操作困难,不适合行ILIF手术。3、ILIF手术术后生物力学稳定性有所下降但优于TLIF手术;ILIF附加同侧椎弓根钉固定与附加双侧椎弓根钉固定生物力学稳定性相当,使用ILIF术式附加侧同椎弓根螺钉固定,可提供较好的即刻稳定性。4、ILIF手术及附加同侧椎弓根钉固定是一种安全、简便、有效的手术方式,在减少椎管内并发症方面具有优势。5、ILIF手术可获得较好的融合率,其融合情况从组织学观察稍好于TLIF手术。

The results showed that there were inadaptive responses,and there were significant differences (P<0.01) among all levels. There were less inadaptive responses in the secondary nursing students than in the baccalaureate standard(P<0.05) and college nursing students (P<0.05); The Basic Physiologic Needs and the Role Mastery were not significantly difference among different level nursing students (P>0.05); The difference in the self-Concept and in the interdependence was significant (P<0.01). There were less inadaptive responses in the secondary nursing students than the BSN(P<0.05) and college nursing students (P<0.05) in the Self-concept; there were more inadaptive responses in the BSN than that in the college (P<0.05) and secondary(P<0.01) nursing students,and the difference was not significant among the college nursing students and secondary nursing students in the Interdependence.

结果表明:不同层次护生在毕业实习期均存在不适应反应,不同学历间有显著差异(P<0.01),中专护生不适应反应少于本科(P<0.05)及大专护生(P<0.05),在生理需要及角色功能方面,不同学历间无显著性差异(P>0.05),在自我概念方面,不同学历间存在显著性差异(P<0.01),中专护生少于本科(P<0.01)及大专(P<0.05)护生,在互相依赖方面,不同学历间存在显著性差异(P<0.01),本科护生多于大专(P<0.05)及中专(P<0.01)护生,大专与中专护生间无显著性差异(P>0.05)。

Except 58 cases missed and 5 cases induced abortion, we followed up 163 cases(241 kidneys) in different gestational age and postnatal period. Among them, 209 kidneys (86.72%) were discovered pyelectasis between 5mm to l0mm. The pyelectasis of 30 kidneys(12.45%) was between l0mm to l5mm, and that of 2 kidneys(0.83%) was thicker than 15mm. Follow-up result is as follow: 137 cases(214 kidneys) were recovered in normal in fetus period or postnatal 15 months. Their pyelectasis was thinner than 5mm(88.80%). Among them, 16 cases(2l kidneys) were recovered in fetus period(8.71%). 121 cases(193 kidneys) were recovered in postnatal 15 months(80.09%). The rest 20 kidneys' pyelectasis was between 5mm to l0mm(8.30%). 4 kidneys' pyelectatis was between 10mm to l5mm(1.66%), and 3 kidneys' pyelectasis was thicker than l5mm(1.24%).

结果:发现一侧或双侧肾盂扩张≥5mm的胎儿有226例,除58例失访,5例引产外,对163例241只肾脏在不同孕龄及出生后的孩子进行了随访。241只肾脏按肾盂扩张在5~10mm间2的只(占86.72%),在10~15mm间30只(占12.45%),≥15mm者2只(占0.83%)分组观察,随访结局如下:137例214只肾盂在胎儿期或孩子出生后15个月内恢复正常,肾盂扩张<5mm(占88.80%):其中16例21只肾盂在胎儿期恢复至5mm以下(占8.71%),121例193只肾盂在孩子出生后15个月内恢复至5mm以下(占80.09)。20只肾盂扩张在5~10mm间(占8.30%)。4只肾盂扩张在10~15mm间(占1.66%)。3只肾盂扩张≥15mm(占1.24%)。

The result showed that the dosage of 5000 IJs/plant was the best one among the 8 dosages, the average of injured legume (3.l%) with BPS was significant lower than that of 40% isofenphos-methyl solution (9.0%) and than that of tap water (53.2%). The rate of grub decrease (95.7%) and the dry weight of peanut (5941.5 kg/ha) with BPS were significant different (P.05) to that of with 40% isofenphos-methyl solution (82.7%, 303.8 kg). The application of BPS was better with irrigation than with spray. At the dosage of 5000 IJs/plant, the combination of BPS and Rhabditis sp.(4500:500) did not show conflictive, coordinate or synergistic effect to grub control.

结果显示:在8个处理剂量中,以5000 IJs/穴的剂量田间效果最好,花生荚果受害率(3.1 %)显著低于40 %甲基异柳磷对照的(9.0 %)和清水对照的荚果受害率(53.2 %),蟒螬减退率(95.7%)和平均产量(5941.5 kg/hm^2)也与40%甲基异柳磷对照的(82.7%, 303.8 kg)有显著差异(P.05);灌施效果显著好于喷施;在5000 IJs/穴的剂量下,混合喷施BPs线虫和小杆线虫(4500:500),未表现出相克、协同或加性效应。

The lowest surface tension of N_xB solution was better at 25℃, and lowest surface tension would down with carbon . cmc( critical micelle concentration) and γ_ in N_(12)B are 4.1 × 10~(-4)mol/L, 24.5 mN.m~(-1),what was smaller than LAS . The cmc of N_(12)B- NaCl are down with NaCl up.The surface tension and cmc of N_(12)B and N_(16)B are higher than that of N_(12)OB and N_(16)OB because of hydrophilic group ,but that of N_(12)B is lower than that of N_(16)B because of hydrophobic group .5. The borate structure and semipolar bond were studied in different pH by infrared spectrum .It indicated that N_xB and N_xOB have not semipolar bond at pH

4、25℃,N_xB系列都有较低的表面张力,并随碳原子数的增加而最低表面张力下降。N_(12)B比同碳原子数的十二烷基苯磺酸钠的最低表面张力34.5mN/m低,且临界胶束浓度为4.1×10~(-5)mol/L,也比LAS低,且随着NaCl浓度的增加而略有下降,比较N_(12)B和N_(16)B,由于C_(16)疏水基的异构体使N_(12)B的临界胶束浓度和临界胶束浓度时的表面张力γ_(cmc都低,由于增加了亲水基团使得N_(12)OB和N_(16)OB的表面张力和临界胶束浓度都要高于N_(12)B和N_(16)B。

It was showed that pectenolone and pectenoxanthin were all found in Gonads, hepatopancreas, mantle and gill, among of them, ovary, hepatopancreas and gill possessed more carotenoid than other tissues, but no carotenoids were found in the white muscles of Yesso scallops. Hepatopancreas contains a variety other carotenoids some kind of other not identified beside pectenolone and pectenoxanthin. The result also showed that the Yesso scallop with orange muscle had more the carotenoids in a variety of tissues than the common, and the female more than the male.4 Impacts of carotenoid accumulation on morphological structure and chemical composition in orange muscle of Yesso scallopThere was no difference between the orange and white muscle obserced by conventional HE staining, while musclular fiber type and matrix density in the orange muscle was different from white ones by the TEM observation of ultra-thin slices, and there were three musclular fiber types, among of them, the loose muscular fiber was orange muscle specifically.

利用HPLC法对橘红/白色闭壳肌虾夷扇贝各组织中含有的类胡萝卜素种类和含量,结果表明,虾夷扇贝在性腺、肝胰腺、外套膜、鳃丝中均含有类胡萝卜素pectenolone和pectenoxanthin,白色闭壳肌中未检测到类胡萝卜素,其中肝胰腺除上述两种类胡萝卜素外,还含有其他多种未鉴定的类胡萝卜素;在各组织中,雌性性腺、肝胰腺、鳃丝中类胡萝卜素的含量较高;橘红色闭壳肌虾夷扇贝各组织中的类胡萝卜素含量高于白色闭壳肌虾夷扇贝;雌性虾夷扇贝中的类胡萝卜素的含量高于雄性。4累积类胡萝卜素对虾夷扇贝闭壳肌的形态结构和化学成分的影响本文对橘红色闭壳肌进行了常规HE染色切片观察,并未观察到异常现象;超薄切片透射电镜观察发现橘红色闭壳肌在肌纤维类型及基质密度等方面有别于白色闭壳肌,在橘红色闭壳肌中有三种肌纤维,其中疏松型肌纤维为橘红色闭壳肌特有。

Using bubble oxygenator, ecc time more than 180minutes , pressure before the artery filter higher than 240 mmhg and haematocrit in the first blood gas analysis more than 27% were significantly different.

使用鼓泡肺,ecc时间大于180 min,及泵压大于240 mmhg组,术中第一次血气分析血球压积大于27%三组,p.05,有显著差异。

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I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里,举止就文雅一些,因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计,这件高贵的制服是香港本土制造,是我们第一套不配帽子的制服。

Do not 'inflate' your achievements and/or qualifications or skills .

不要 '夸大' 你的业绩或成果,条件或者技能。