查询词典 difference sequence

An arithmetic progression of primes is a sequence of primes with a common difference between any two successive numbers in the sequence.

在一个等差数列，任意相邻两项的差都是相等的。

Primers used in PCR were designed based on the sequence of mitochondrial 16S rDNA (AJ250642 and AF312718) and beta-actin (AY910691) of Eriocheir sinensis which had been deposited in GenBank. PCR analysis using total DNA from muscle, branchia, testis and sperm indicated that 16S rDNA exhibited different expression in the four samples. Beta-actin gene was detected plentiful in all the four tissues/cells and exhibited the same expression pattern. 16S rDNA gene amplification was detected at high levels in muscle and branchia, appreciable low in testis and very low in sperm. The PCR products of 16S rDNA gene were sequenced and aligned with the sequence of 16S rDNA from GenBank (AJ250642 and AF3 12718), and the alignment result showed there was no difference between them.

通过GenBank中的中华绒鳌蟹线粒体16S rDNA序列设计引物，利用PCR扩增方法，以beta-actin做内参，检测了中华绒螯蟹肌肉、鳃、精巢和精子中线粒体16S rDNA扩增情况，发现各组织或细胞beta-actin扩增片段大小、条带宽度和亮度基本一致，表明各模板DNA量基本一致；而在同一DNA浓度下，16S rDNA的扩增片段长度虽一致，但其产物量存在明显差异，精子16s rDNA产物量显著低于其他3种组织，其条带宽度和亮度很弱；16S rDNA扩增产物经测序分析及比对证明与已有序列完全吻合。

New inspection method was deduced by using constant coefficient of variation in sequence inspection method, and the approximate relationship of inspection variables between mean method and sequence one was gained by analyzing difference of two methods.

将变差系数不变的条件引入到序贯检验方法中，推导出新的结构强度检验方法，分析检验变量与均值法的差异，得到两者的近似关系。

As the RFID system has its own characteristics, there has great difference between location sequence and traditional sequence.

由于RFID系统的特殊性，位置信息构成的序列与传统的序列有很大的差别。

RT of the all age groups of students do not have significant difference; But in sequence learning, participation degree of consciousness and abstraction amount of sequence knowledge among various groups is different. Sequence learning of primary and junior high school students is implicit, and that of college students has participation of consciousness.

大中小学生的序列反应时没有显著差异；但是，在序列学习中，不同年龄组学生意识的参与程度和序列知识的提取量是不同的，小学生和初中生的序列学习以内隐性为主，大学生的序列学习则有意识成分的参与。

RDNA-ITS sequence analysis of 30 populations showed that there is difference among M. incognita, M. hapla, M. arenaria, M. javanica, M. panyuensis, M. zhanjiangensis. and M. hainansis, whereas little rDNA-ITS sequence difference among three normal Meloidogyne species.

通过对30个种群的rDNA-ITS序列分析，发现番禺根结线虫、海南根结线虫、北方根结线虫、湛江根结线虫、南方根结线虫、爪哇根结线虫和花生根结线虫种间的DNA-ITS区碱基序列存在差异，但后3种常见种之间差异小。

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

Sequence similarity of gyrB sequence from twelve Gordonia type strains ranged from 79.3% to 97.2%, corresponding to 270-41 nucleotide differences while there was only 0.3-3.8% difference in 16S rRNA gene sequence similarity at the interspecies level.

本研究之结果将可利用gyrB基因作为Gordonia菌种快速鉴定之依据，若两菌株之16S rDNA序列相似度大於99.7%，gyrB基因相似度大於97.2%，则判定两菌株为相同菌种。

Complete genome sequences of 20 viruses in Bunyaviridae loaded from NCBI were analyzed by two biological softwares of DNAMAN and DNASTAR. The results indicated great variability in both nucleic acid sequence and protein structure between plant viruses and animal viruses:(1) only plant viruses could encored NSm in M genome;(2) the length of nucleic acid sequence and protein sequence was different;(3) GC content of nucleic acid of animal viruses was higher than plant viruses of that;(4) protein topology analysis by online software SMART discovered that there was significant difference for the structure of glycoproteins G(subscript nG between animal-infecting and plant-infecting viruses;(5) usually, protein of plant-infecting virus had more complicated structure, more low compositional complexity and had N-Signal peptides except INSV.

本文从NCBI数据库下载具有完整基因组序列的布尼亚科病毒的5个属20种病毒的序列，用生物学软件DNAman，DNAstar进行比对分析，发现布尼亚科病毒属中的植物病毒在核酸序列及蛋白结构上与动物病毒有很大差异：(1)只有植物病毒的M基因组能编码NSm运动蛋白；(2)植物病毒和动物病毒在核酸序列和蛋白质序列长度均有差异，表明该科病毒是进化速度较快的病毒；(3)植物病毒核酸序列的GC含量低于动物病毒；(4)通过SMART网络软件进行蛋白质拓扑结构分析发现植物病毒和动物病毒在糖蛋白GG的结构上存在显著差异；(5)植物病毒糖蛋白结构较为复杂，有较多的紊乱区域，除INSV外，其他病毒都具有N端信号肽。

After amplying a 2.2kb fragment form the PPV-SC1 RF-DNA,we clone the fragment into pMD 18-T,named pTNSl.The whole sequence which is 1989 bp long was determined by sequencing, including the complete ORF of PPV-SC1 NS1 which encoding 662 amino acids.Alignment of pairs of sequence indicates that there are 98% and 99% similary with other porcine parvovirus strains Kresse and NADL-2, respectively. Multiple sequence alignment discloses that there are a few difference between ppv-scl nsl gene and other ppv nsl gene: A-G at 39nt,T-C at 153nt,A-G at 175nt, A-C at 1117nt, A-C at 1535nt .Alternative codon in ppv-scl nsl have distinctly different frequentfy by codonbias analysis at EMBOSS(http://genopole.toulouse.inra.fr/bioinfo/emboss). Thereis not distinct hydrophobicity and transmenbrane helices in ppv-scl nsl protein. Struction domain anslysis of PPV-SC1 NS1 protein indicate that there are a ATP/GTP-binding site motif A at 398-405,16 Protein kinase C phosphorylation site,21 Casein kinase II phosphorylation site,and 3 cAMP/cGMP-dependent protein kinase phosphorylation site.At the same time ,there is a same motif between ppv-scl nsl and Poxvirus D5 protein-like which may share in the same fuction which is necessary during virion duplication.

将PPV-SC1 NS1序列与其他PPV NS1基因进行多序列比对，结果显示，PPV-SC1 NS1与其他的PPV NS1的同源性较高，仅存在个别的差异，分别是第39位A→G，第153位T→C，第175位A→G，第1117位A→C，第1535位A→C；同源搜索比较表明，PPV-SC1与PPV NS1同源性可达98％、99％，与其他的细小病毒NS1基因也存在很大的保守性；密码子偏向性分析结果表明PPV-SC1 NS1基因在同一氨基酸的不同密码子的选择上存在一定的偏向性；PPV-SC1 NS1蛋白总体上说具有亲水性不存在明显的疏水性区段，用swiss TMPRED软件预测PPV-SC1 NS1的跨膜区，返回的结果并没有得到有显著意义的跨膜区的存在；根据基于motif数据库的结构域预测，PPV-SC1 NS1的第393-415位氨基酸残基存在潜在的ATP／GTP结合位点，该蛋白还存在16个蛋白激酶C磷酸化位点，21个酪蛋白激酶2磷酸化位点，3个cAMP-／cGMP依赖蛋白激酶磷酸化位点，PPV-SC1 NS1蛋白与POX_D5(痘病毒D5蛋白)具有一致的保守结构域，推测NS1可能与POX_D5有类似的功能。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。