查询词典 devise

Devise a pair of primer to put out the section of pgE which code the dense antigen episode, clone into pET-32a, name the masculine plasmid with pET-32pgE. Transform the BL21 strain with pET-32pgE, choose several single bacterium drops at random and contaminate 2X YT. vibrate at 37癈 over night. Contaminate another tube with the above culture to the final concentration of 2%, continue to culture to OD6oo0.6, add IPTG to the final concentration of Immol/L. inducing 6 hours. Analyse the expression with SDS-PAGE and Western-blot, the result is that the expression has actually happened and the product has immunoreactivity.

设计一对引物从gE全基因克隆载体pgE中调出抗原决定簇密集编码区，克隆进PET-32a表达载体，双酶切和PCR鉴定出阳性者，命名为PET-32pgE，转化BL21菌株，挑取单菌落在加有氨苄的2×YT培养基中37℃过夜长至饱和，按2％体积转种另一管，培养至OD_(600)为0.6，加IPTG至终浓度1mmol／l，诱导4～6小时，SDS-PAGE和Westernblot分析，证明确实表达并具有免疫反应性。

Confirm the establishment quantity and erection site of on-the-spot warning devise, such as the manual alarm button with telephone jack、indicator of rivers and hydrant button of fire prevention and so on, and also fire broadcasting system and fire telephone system.

确定带电话插孔的手动报警按钮、水流指示器、消火栓按钮等现场报警设备以及消防广播、消防电话的设置数量和安装位置等。

And no sooner had Joan, his handsome, shallow-minded wife, the easy-going mother of his many children, heard of her exalt ed estate than her romantic soul began secretly to devise a brilliant and fitting alliance for her beautiful young daughter.

他的妻子琼是一个健壮、浅薄的女人，也是孩子们随和的母亲。当她一听说自己高贵的地位时，便立刻想入非非，暗自打算为她的年轻漂亮的女儿找一个显赫、门当户对的婆家。

And no sooner had Joan, his handsome, shallow-minded wife, the easy-going mother of his many children, heard of her exalted estate than her romantic soul began secretly to devise a brilliant and fitting alliance for her beautiful young daughter.

他的妻子琼是一个健壮、浅薄的女人，也是孩子们随和的母亲。当她一听说自己高贵的地位时，便立刻想入非非，暗自打算为她的年轻漂亮的女儿找一个显赫、门当户对的婆家。

In order to evaluate the solution quality obtained by algorithm SB and compare its resulting backbone set with the optimal one, we devise an algorithm OOB (standing for Obtaining Optimal Backbones) to obtain the optimal solution.

为了评估演算法SB所得到的结果，我们设计了一个演算法OOB（全名为Obtaining Optimal Backbones）来得到最佳解。

Whether in using technology to devise accurate timepieces for athletes, or sleek artistic creations, Tissot watches are the must have for anyone with a desire for the best in quality and design.

无论是在利用技术来制订的运动员，还是光滑的艺术创作准确的手表，天梭手表，是任何人都必须有一个在质量和设计最好的愿望。

Agronomists are beginning to devise tools to help monitor the efficiency of water use.

农业学家们开始设计工具，帮助监测水的使用效率。

Agronomists are beginning to devise tools to help monitor the efficiency of water use.

农业专家们正开始想办法制造一些设备来帮助检测水资源的使用效率。

Agronomists are beginning to devise tools to help monitor the efficiency of wa ter use.

农业学家们开始设计工具，帮助监测水的使用效率。

It really is extraordinary that after all these years, educationists have still failed to devise anything more efficient and reliable than examinations.

考试在我们的社会中是成功或失败的标志。在具有决定意义的某一天，你的一生可能就被决定了。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。