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In this paper, we studied M. meretrix ferritin, cathepsin B and caspase genes, which are involved in clam larval shell formation, nutrition, metabolism and apoptosis, respectively. We have cloned the three genes, investigated the temporal and spatial expression profile both at gene and protein level in trochophore (L1), D-veliger (L2), pediveliger (L3) and postlarvae (L4). The potential roles of these proteins were analyzed with specific inhibitors during larval development. Firstly, embryos were found developed into trochophore-like larvae with no shell if cultured at gastrula stage in artificial seawater without iron. Shell-like structures were formed only in the presence of iron. The larvae which had been transferred at L1 stage into ASW developed normal shell. This indicated that iron and iron associated protein are important for larval shell formation. The EST sequence which is homologous with ferritin, which is a principal iron metabolic protein, was selected from the M. meretrix cDNA library. The full-length of ferritin subunit cDNA was cloned by RACE. The results of real-time PCR revealed that the MmeFer mRNA expression changed before and after the larval shell formation.

本论文以文蛤幼虫为研究对象，分别对文蛤幼虫发育过程中贝壳形成相关的铁蛋白、营养及变态相关的组织蛋白酶B及变态过程中细胞凋亡相关的caspase三个基因进行了克隆，分析了基因及编码蛋白在担轮幼虫期(L1)、D形幼虫期(L2)、壳顶幼虫期(L3)和稚贝期(L4)的时空表达特征，解析了其可能的功能，并研究了相应酶类的特异性抑制剂作用对幼虫发育过程的影响，进行了目标蛋白的功能验证，详述如下：研究结果显示，在文蛤胚胎发育到原肠胚时放入不含铁离子的人工海水中培养，发育成无壳的畸形，随着人工海水中铁离子添加浓度的升高，幼虫长出壳状组织接近正常状态；而发育到L1期幼虫放入不含铁离子的人工海水中培养却可以发育出正常的壳，推测铁和铁代谢相关蛋白在幼虫贝壳初始形成有重要的作用。

Based on the finite strain theory, a subroutine‐UEL for ABAQUS is developed , and the residual pore pressure under wave load is computed, and stability of soil around pipeline and seabed is analyzed. Regarding the liquefaction soil as model, a subroutine‐UMAT for ABAQUS is developed , and the behavior of pipeline in liquefaction soil analysis qualitatively.

并据此模型对复杂波浪荷载下埋置管线的动力响应进行分析，发现波浪的非线性和随机性以及管线-土体之间的接触效应对计算结果的影响十分显著，如果忽略这些因素，则会在分析埋置管线动力响应时造成较大误差。4。

Article 38 The state encourages units and individuals in the development of unexploited land in accordance with the overall planning for land utilization and under the prerequisite of protection and improvement of the ecological environment, prevention of water and soil erosion and land desertification; the land suitable to be developed into agricultural land should be developed into agricultural land on a priority basis.

第三十八条国家鼓励单位和个人按照土地利用总体规划，在保护和改善生态环境、防止水土流失和土地荒漠化的前提下，开发未利用的土地；适宜开发为农用地的，应当优先开发成农用地。

Although problems and limitations remain, management science has developed as an effective means for solving tedious but limited problems. Algorithms, or exact procedures for optimum solutions, have been developed for problems that are well defined or repetitious, and where a universal unidimensional cardinal scale utility, such as money, can reasonably be used as a measure of the desired outcome.

虽然一直存在着缺陷和问题，但是科学管理已经发展成为解决复杂问题的很好方法，种算法，或者是一种最优选择的方法，已经发展为解决问题的一种很好的界定或者反复的工作模式，是一种单一维度和协和效用尺度，例如金钱就可以经常用来衡量产出。

A suit of software used for boiler's cold and hot tests is developed by EXCEL 2000 and VB6.0, which can process all the data and cartography during the tests. The software can print test report in a certain extent. Many universal ActiveX control and Class are developed. The experiences on the software are summed up. At last, the direction of the software's development used for boilers' tests is forecasted.

依据试验原理与方法，以 Excel 2000和 VB6.0为开发工具，开发了一套针对四角切圆燃烧锅炉的冷、热态性能试验通用计算软件，该软件能够完成试验过程中所有数据的处理和图表、曲线的绘制，并具有一定的报告自动打印功能；制作了可通用的 ActiveX 控件和类，并对软件开发的经验进行了总结；最后，对锅炉性能试验软件的未来发展趋势进行了展望。

Medical examination in China which developed along with the upgrowth of medical education originated in Xizhou period and developed in Tang and Sui Dynasty.

我国医学考试起源于西周时期，形成和完善于隋唐，此后随着医学教育事业的兴起而不断发展。

The "crystal sea" brand series stainless steel vacuum flask and coffee pot complete series has been developed and mass production, the company developed are initiated research and development of new projects.

其中"晶海"牌不锈钢保温瓶系列及咖啡壶系列已研制完成并批量生产，公司研制人员正着手研究开发新的项目。

This kind of notation seems to have developed no earlier than the eight century , but by the ninth it was firmly established as the primary method of musical notation. The basic notation of the virga and the punctum remained the symbols for individual notes, by other neumes soon developed which showed several notes joined together.

纽姆谱标识的种类似乎在18世纪后才得以发展，但是在19世纪它已稳定的奠定了作为音乐记谱首要谱法的地位，virga和punctum仍然是在为单独的音符做标识，但是不久后，另一种可以可以为几个连在一起的音符做标识的纽姆谱得到了发展。

It is important to developed of cross wedge rolling techniques that the software package has been developed.

该系统的开发、研制成功，对楔横轧技术的发展与推广具有重要的推动作用。

The research of report of our country wind and engineering capability and developed country photograph are older than having difference, great majority wind-force sends electric field is to use the loan of the developed country to buy foreign equipment, dimensions small, cost is high.

我国风电的研究和制造能力与发达国家相比有较大差距，绝大多数风力发电场都是利用发达国家的贷款购买国外设备，规模小、成本高。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。