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Design another set of specific primers, of which upstream and downstream primers were located in the two sides of the cleavage site respectively, to develop the one-step RT-PCR method for detecting H5 and H9 subtypes of AIV. Identify the sensitivity of the developed one-step RT-PCR method by detecting 2 AIV strains of H5 subtype and 3 AIV strains of H9 subtype, and the specificity by detecting the RNAs of NDV, IBV and IBDV as well as analogue detection using computer software.

根据H5、H9亚型HA基因序列,分别设计一套特异性引物,其上、下游引物分别位於裂解位点两侧,应用RT-PCR一步法检测AIV H5和H9亚型,并验证所建立方法的敏感性;通过检测NDV、IBV和IBDV等RNA病毒及电脑软件模拟检测,验证其特异性。

The main purpose of this thesis is to recommend the analogy to develop trends .

本论文的主要目的是介绍类比的一些发展动态。

We must do our best to develop the student's ability to analyse and solve problems.

我们必须尽力培养学生分析问题和解决问题的能力。

The company website operation, develops the B2C service to develop channels and so on Carrefour, Bestbuy to complete to Southeast Asia market survey Region marketing consultant works the responsibility division key sales territory, investigates the market environment and the domestic customer consumer behavior development essential channel seller, formulates the region sales program formulation reasonable price strategy, the opportune moment carries on the effective promotion customer maintenance, utilizes the actual seller the 4C theory to train The main achievement completes TOYOTA the SALE training (behavioral science, marketing theory, CS, brand management), grasps Toyota Production System and Just in the time management implements the Toyota type sale, manages in the region the channel, the achievement month by month promotes, to achieve the region year sales target (70,000,000 RMB) The commodity business planning works the responsibility deliberated company's profit structure, the analysis with the control division old times the profit and loss, the formulation new product operational policy, the strategy market environment investigation estimate potential market profit rate comparison product competition situation, the product localization, the estimate life cycle, the curve analytic product cost, after formulation price strategy product going on the market, track produce market situation The main achievement participates in W-PJT, NOVA-PJT, the AIR-FORCE project, under the project participates in the personnel joint effort, the product year sales volume achieves 100,000 Commodity business planning work responsibility market survey; Coordinates the South Korean research and development personnel, to the new product the brand-new development, formulates the zone price strategy, compared with competitive power situation.

公司网站运作,开拓B2C业务开发Carrefour、Bestbuy等渠道完成对东南亚市场调查区域销售顾问工作职责划分重点销售区域,调查市场环境及当地客户消费行为开发关键渠道销售商,制定区域销售计划制定合理价格策略,适当时机进行有效的促销活动客户维护,将4C理论运用到实际销售员培训主要业绩完成TOYOTA SALE培训(行为科学、营销理论、CS、品牌管理),掌握Toyota Production System和Just in time管理实施Toyota式销售,管理区域内渠道,业绩逐月提升,达成区域年销售目标(7千万RMB)商品企划工作职责与管理部研讨公司的利润结构、分析往年损益,制定新品经营方针、策略市场环境调查估计潜在市场获利率比较产品竞争情况,产品定位,预估生命周期、曲线分析产品成本,制定价格策略产品上市后,跟踪产品市场情况主要业绩参与W-PJT、NOVA-PJT、AIR-FORCE项目,在项目参与人员共同努力下,产品年销量均达到10万台商品企划工作职责市场调查;协同韩国研发人员,对新产品全新开发,制定区域价格策略,比较竞争力情况。

Combining the teaching practice of analytical geometry, this article puts forward the implement strategy of instruction of mathematics study: penetrate the principle of mathematics study towards the students; instruct the students how to study; help the students form the better non-intellect factor; develop the student's original cognizance level; present the instruction of mathematics study ; raise the extent of students' participation ; keep the teaching and instruction in harmony and instruct the solution of students' query.

结合立体几何教学实践,本文提出了数学学法指导的实施策略:向学生渗透数学学习方法理念:对学生学习的各环节进行学习方法指导;对学生形成良好的非智力因素进行指导;发展学生的元认知水平;数学学法指导的基本方法;提高学生的智力参与程度;教学与学法指导和谐统一;对学生质疑问难的方法进行指导。

This course is designed to develop your knowledge and understanding of financial management and build on your research and analytical skills.

本课程的目的是发展自己的知识和理解的财务管理和建设的研究和分析技能。

This essay mainly focused on the analyzation and discussion of how to enhance the management of science and technology information of high education , and to develop and utilize the resource of science and technology information reasonably, straining to make full use of it, to make high education to be the collecting and distributing center of science and technology information, boosting the development of economic society of the nation and the region and cultural advancement.

决定一个国家、一个地区经济高速发展的因素之一,就是在于具有丰富信息资源,并能充分利用信息的作用。如与我国一衣带水的日本是一个资源缺乏、土地面积狭小的国家,但是由于重视开发与利用信息

And this paper mainly introduce the empolder circs of the monopolistic store, the basic design anamnesis the develop circumstance of the system and the application circs in actual.

本文主要介绍了专卖店系统的开发情况,基本设计思想、系统开发环境及目前的应用情况。

It sounds like a great name. I've missed AnAn dearly, every now and then I would take a look at the photos and some of the clips that we took using my mobile phone. FangFang will be back in the next few days, we'll develop the photos for grandma and grandpa, can you please send over a few photos of your whole family?

很好听,好想安安的,没事就看看照片和用手机拍的短暂镜头,这几天方方回来,我们会把照片洗出来带给爷爷奶奶看,你也可以再发几张你们的全家福,还有安安铁蛋小时侯可爱的照片,我去给洗出来,爷爷奶奶看到会很高兴的。

In accordance with the lecturer, the Anasazi could develop thetechnology of diversion dams and canals to irrigate farmland and store crop.

模版不是不能用的,危险的就是模板的语言和文章其他的部分语言差距很大~这一篇文从逻辑到用词到整体的语言运用,都是很好的范例,大家可以学习一下。

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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。