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The profile of liver CYP450 induction by DEX in male rat After 4 daily i.p. administering of 0,25, 50 and lOOmg/kg/d DEX in male Wistar rat, it was determined the total CYP450 content, the mRNA and protein expression levels of CYP3A1, CYP3A2 and CYP2B1/2, and the ERD (CYP3A catalytic activity), PROD (CYP2B catalytic activity) and BROD (total CYP450 catalytic activity) in livers.

DEX对雄性大鼠肝脏CYP450的诱导效应雄性Wistar大鼠腹注0、25、50和100mg DEX/kg/d诱导处理4天后,测定大鼠肝脏总CYP450含量,CYP3A1、CYP3A2和CYP2B1/2的mRNA及蛋白表达水平,肝脏红酶素脱甲基酶(ERD,CYP3A活性),苄氧基试卤灵脱乙基酶(PROD,CYP2B活性),苯氧基试卤灵脱乙基酶(BROD,总CYP450活性),结果表明,CYP450含量、ERD、PROD和BROD在DEX多次诱导后都有升高,CYP3A1 mRNA表达水平、蛋白含量和酶活性有明显的升高,剂量效应关系明显;CYP3A2蛋白也有明显升高,而其mRNA表达水平却没有变化,提示CYP3A1和CYP3A2的诱导机制可能不同。

The profile of liver CYP450 induction by DEX in male ratAfter 4 daily i.p. administering of 0,25, 50 and lOOmg/kg/d DEX in male Wistar rat, it was determined the total CYP450 content, the mRNA and protein expression levels of CYP3A1, CYP3A2 and CYP2B1/2, and the ERD (CYP3A catalytic activity), PROD (CYP2B catalytic activity) and BROD (total CYP450 catalytic activity) in livers.

DEX对雄性大鼠肝脏CYP450的诱导效应雄性Wistar大鼠腹注0、25、50和100mg DEX/kg/d诱导处理4天后,测定大鼠肝脏总CYP450含量,CYP3A1、CYP3A2和CYP2B1/2的mRNA及蛋白表达水平,肝脏红酶素脱甲基酶(ERD,CYP3A活性),苄氧基试卤灵脱乙基酶(PROD,CYP2B活性),苯氧基试卤灵脱乙基酶(BROD,总CYP450活性),结果表明,CYP450含量、ERD、PROD和BROD在DEX多次诱导后都有升高,CYP3A1 mRNA表达水平、蛋白含量和酶活性有明显的升高,剂量效应关系明显;CYP3A2蛋白也有明显升高,而其mRNA表达水平却没有变化,提示CYP3A1和CYP3A2的诱导机制可能不同。

Trace bromate in drinking water was determined by ion chromatography with suppressed conductivity detector.

摘 要:采用抑制电导离子色谱法检测饮用水中的痕量溴酸盐。

The kinetic parameters of vanadium catalytic oxidation of azomethine H by bromate have been determined by using spectrophotometric method .

以酸性介质中痕量钒催化溴酸钾氧化甲亚胺 H显色反应为对象,利用光度法研究了反应的动力学参数。

Methods: The method used direct sampling technique to determinate chloroform, bromodichloromethan, dibromochloromethane, bromoform, which was seperated by tube column, detected by electronic capture detector and determined by inner standard method.

水样直接进样,用毛细管柱色谱分离,电子捕获检测器检测,采用内标法定量,可同时测定水中三氯甲烷、一溴二氯甲烷、二溴一氯甲烷和溴仿。

Methods: The twelve volatile halogenated hydrocarbons (1, 1-dichloroethene, dicloromethane, trans-1, 2-dichloroethene, cis-1, 2-dichloroethene, chloroform-1, 1, 1-trichloroethane-1, 2-dichloroethane, tetrachloromethane, bromodichloromethane, dibromochloromethane, tetrachloroentene and bromoform) in drinking water were determined by headspace capillary gas chromatography.

采用顶空毛细管柱气相色谱法测定饮用水中1,1-二氯乙烯、二氯甲烷、反-1,2-二氯乙烯、顺-1,2-二氯乙烯、三氯甲烷、1,1,1-三氯乙烷、1,2-二氯乙烷、四氯化碳、一溴二氯甲烷、二溴一氯甲烷、四氯乙烯及三溴甲烷等12种挥发性卤代有机物。

Intestine, lung, heart, kidney and liver tissue samples were also collected after reperfusion 2 hours, and (1) protein concentration of bronchia alveolus lung fluid ,(2)HSP70 expression in heart and kidney (3) histology pathology and (4) oxidation injury (MDA and GSH-PX) in above tissues were determined.

休克2小时,收集小肠、心肌、肺、肝、肾等组织用于测定(1)支气管肺泡灌洗液的蛋白含量(2)免疫组化检测心脏和肾脏HSP70的表达(3)组织病理切片(4)组织抗氧化指标。

Methods Serum TNF-2 and NO/NOSlevels were determined in 42 patients with children broncho pneumonia both before and after treatment as well as in 35 controls.

方法分别应用放免法和化学法测定了42例小儿支气管肺炎患者血清NO/NOS和TNF-2含量,并与35名正常儿童作比较。

The percentage of Clara cells in the bronchiolar epithelium and PMN was determined in the broncho alveolar lavage fluid.

目的 探讨机械通气对大鼠肺组织中CC10蛋白表达的影响及CC10对肺炎症反应的影响。

Methods Serum IGF-Ⅱ, IL-6 levels were determined with RIA in 40 patients with child bronchopneumonia both before and after treatment as well as in 30 controls.

应用放射免疫分析对40例支气管肺炎患儿进行了血清IGF-Ⅱ、IL-6水平检测,并与30名正常健康儿童作比较。

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To Be Determined
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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。