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Methods:The conservative region of mTOR gene was inserted into pLXIN reversedly, then the vector was packaged in PT67 cells by transfection with lipofectamine and transfected to VSMC. The efficiency of antisense inhibition was verified,and the changes of p70S6k and 4E-BP1 were also determined at the same time. The proliferation activity was determined by flowcytometry and MTT.

采用脂质体介导转染包装细胞后构建mTOR的反义重组逆转录病毒载体(pLXIN-AmTOR)感染VSMC，检测mTOR及其下游底物包括真核细胞启动子4E结合蛋白1(4E-BP1)和核糖体蛋白S6激酶(p70S6k)的表达变化，以及VSMC细胞增殖周期和增殖活性的改变。

Additionally, 75 volatile chemical constituents have been identified from the petroleum ether extracted part by capillary GC-MS, and the relative percentages of them are determined by GC method. The result showed that the volatile constituents of Musella lasiocarpa mainly includes advanced alkane, aliphatic acid and ester, aldehyde, ketone, aromatic and its heterocyclic compounds. With the compounds that have been isolated and identified from Musella lasiocarpa, the biogenensis pathway of new compound MT-3 is approached. Furthermore, the bacteriostasis of the 6 compounds to Escherichia coil, Pseudomonas aerusinosa, Bacillus megaterium, Sarcina lutea and Bacillus subtilic are determined by flat dilution method.

此外，从其石油醚萃取部分用毛细管色谱法分析鉴定出75种挥发性化学成分，并测定了各组分的相对百分含量，结果表明，地涌金莲挥发性化学成分以高级烷烃、高级脂肪酸酯及醛、酮、芳香和芳香杂环化合物为主；根据从地涌金莲中鉴出和分离得到的化合物，探讨了新结构化合物MT-3的生源代谢途径；采用平板稀释法测定了地涌金莲中六种化学成分对大肠杆菌、绿脓杆菌、巨大芽孢杆菌、藤黄八叠球菌和枯草芽孢杆菌的抑菌作用。

The serumal CRP wae determined with Immunonephelometric method, the IL-6 wae determined with double antibody sandwich ELISA method.

比较每组内治疗前后血清中的CRP和IL-6的变化，及两组间试验终点时间血清中的CRP和IL-6变化。

Moreover, nano-scale SiO〓 particle is as seed, styrene-acrylate-acrylic acid emulsion polymerization and soapless acrylic emulsion are studied from varying the level of SiO〓 particle and reaction temperature, particle size of these emulsions are determined and SiO〓 content of participating polymerization is also determined. Kinetic study is carried out in the presence of nano-scale SiO〓 particle.

此外用原位聚合法制备了纳米SiO〓/苯乙烯-丙烯酸酯复合乳液和无皂丙烯酸乳液，研究了不同SiO〓含量、反应温度等对聚合反应稳定性的影响，测定了所得乳胶粒的粒径，分析了在乳液中SiO〓含量，并进行了在SiO〓存在下乳液聚合反应的动力学研究。

Methods Modified Columbia chocolate agar was compared with blood agar staphy stick for the primary isolation of Hi from sputa. The sensitivities of 20 antibiotics to Hi were determined in vitro by KB method and MICs of 12 antibiotics were determined by broth microdilution method. The clinical characteristics of 92 cases with lower respiratory tract infection by Hi were analyzed.

比较改良哥伦比亚巧克力琼脂与血琼脂加金黄色葡萄球菌划线对Hi的分离率；以Kribry-Bauer法测定Hi对20种抗生素的敏感性，以肉汤微量稀释法测定12种抗生素对Hi的最小抑菌浓度；分析92例Hi下呼吸道感染的临床特点。

The purity of the labelled LL-EPO was determined by both trichloroacetic acid and SDS-PAGE method, and the biological activity was determined by the reticulocyte counting method .

同时比较了双相氯胺-T法和常规氯胺-T法标记LL-EPO、凝胶过滤和离心超滤法分离纯化标记蛋白的效果；并初步探讨了温度对标记蛋白放化纯度的影响。

The inner reorganization energy and the exothermicity of the reaction are then determined. Closed shell HF SCF calculations have been carried out for the systems Tryptophy1-Tyrosine and Tyrosyl-Tryptophan for different values of R, and the Koopmans^,theorem is invoked to estimate the energy level splitting A.From the obtained A~m~i~n, the values of electron transfer matrix element V~D~A are determined to be 0.96kJ. mol^-^1 and 0.87kJ.mol^-^1 for Tryptophyl-Tyrosine and Tyrosyl- Tryptophan, respectively.

对色氨酰酪氨酸和酪氨酰色氨酸体系进行闭壳层HF自洽场计算，按Koopmans定理计算体系分子轨道分裂能值A，在R约为0处发现了A的极小值，从而获得色氨酰酪氨酸及酪氨酰色氨酸体系分子内电子转移的电子转移矩阵元V~D~A分别为0.96kJ.mol^-^1和0.87kJ.mol^-^1。

By considering channel characters and thermal distribution of the rotational levels, experimentally determined absolute photoabsorption spectrum of molecular hydrogen have been analysed: these vibronic oscillator strengths in the region of 11.0―15.0 eV are determined, the total photodissociation of B′1Σ+u is also obtained.

利用通道规律，并考虑了转动分布对跃迁峰型的影响，对测量得到的绝对氢分子光吸收谱进行了分析。文中逐个确定了11.0―15.0 eV各振动态的振子强度，并得到了B′1Σ+u的解离强度。

While other reaction conditions were setted, viscosity and yield of the product P were determined by Ubbelohde viscosity meter in different temperature;By parity of reasoning,viscosity and yield of the product P were determined in different initiator concentration、monomer concentration and monomer ratio.

固定其它反应条件不变，用乌氏粘度计测定了在不同引发剂浓度下产物P的特性黏度及产率；以此类推，测定了在不同单体浓度、单体配比条件下产物P的特性黏度及产率。

Several investigators have determined that placing the electrode parallel rather than perpendicular to the target nerve substantially increases the size of the lesion, thereby reducing the likelihood the treatment will miss or only partially coagulate the target nerve.234,235 After a literature review and cadaveric study, Lau et al.234 concluded the ideal electrode position is across the lateral neck of the superior articular process rather than the groove formed at the angle of the superior articular and transverse processes, as was used in most studies.159,160Other investigators have found the maximal lesion size to be reached within 60 s of lesion time,235–237 independent of whether the system is temperature or voltage controlled.238 Studies conducted in human myocardium have determined that irrigation fluid has either no effect or a slightly beneficial effect on lesion size.239 Hence, the use of LA to prevent procedure-related pain or steroid to reduce the incidence of neuritis184 should theoretically have no adverse effects on the efficacy of radiofrequency denervation.

一些研究者认为，将点击平行的置于目标神经比将其垂直置于目标神经增加了消融的范围，因而减少损伤可能使目标神经不被或部分被消融。Lau等回顾了文献中对尸体的研究，总结出理想的电极放置位置为上关节突颈部外侧而非常采用的上关节突与横突形成的沟。其他学者研究发现，不论系统是温度控制或电压控制，其最大的损伤范围都在60秒内达到。在心肌灌注的研究中，灌注液多损伤范围造成轻微或不造成损伤。因此，使用LA预防过程相关性疼痛或类固醇防止神经炎发生时，需理论上确保对射频消融效果无副作用。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。