查询词典 detection

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

Methods MTT assay for detection of single use of Adriamycin and combination of Adriamycin with gleditsia saponin on the Role of the SMMC-7721 cell survival rate; detection of mRNA expression of bcl-2 by PCR method; detection of protein expression of bcl-2 by Western blotting method.

MTT法检测单独应用阿霉素或阿霉素与皂角皂苷联合作用的SMMC-7721细胞的生存率；RT-PCR检测bcl-2 mRNA表达；Western blotting检测bcl-2蛋白的表达。

Quantum photodetection: direct detection, heterodyne detection, and homodyne detection.

量子光电探测：直接检波，外差检波和零差检波。

The influence of concentration and pH of the mobile phase on the separation and detection was investigated. The elution and separation were carried out with an isocratic mobile phase containing 10 mmol/L NaOH. A quadruple-potential waveform used for the detection was optimized. The detection limit of neomycin was down to 27 ng/mL.

考察流动相浓度和pH对色谱分离和检测的影响，确定色谱分离的最佳流动相条件为10 mmol/L NaOH溶液，并优化新霉素的测定电位和电极的活化电位，获得脉冲安培的最佳四电位检测模式。

According to the meteyard of edge detection and consulting the design-aim of optimal edge-filter, this paper discussed the general rules of selecting the mother function of wavelet used in edge detection, then constructed a quadric B-spline wavelet and presented a method of adaptive thresholds edge detection for image based on wavelet transform.

根据边缘检测的评价标准，参照最佳边缘滤波器的设计要求，确定选择用于边缘检测的小波母函数的一般准则，并在此基础上构造出二次B样条小波，提出了基于小波变换的自适应阈值图像边缘检测的新方法。

Detection of VO_3~- by using promethazine;2. Chlorpromazine as a new inorganic analytical agent—rapid microdetection of bismuth;3. Based on the detection rules of Y802 oven, it makes a supplement to it and puts forward a detailed detection operating sequence.

本文指出了《JJG- 0 70 - 1 999纺织通风式烘箱计量检定规程》存在的问题，并根据作者多年的工作经验，在借鉴 Y80 2型烘箱检定规程的基础上，对其进行了补充、完善，提出了比较详尽的检定操作程序。

4In order to realize the detection of 360°seamless,we proposed the similarbiological modelling"the ommateum"staring detection system.According to the singlelens\' angle of view,we designed the image sensor system of"the ommateum"which uses 6groups of lens and the DSP processing flow.The partial parameter testing experimentindicated that the ommateum system could achieve the performance requirement of the rayradiation detection system.

4为了满足360°无缝探测的要求，提出了类似仿生的&复眼&凝视型探测系统，根据单个镜头的视场角，设计了由6组镜头组成的&复眼&图像传感器系统和DSP处理流程，部分参数测试实验表明复眼系统能满足核爆炸光辐射探测的性能要求。

The characteristics of the variance of the instantaneous velocity for the incident vehicle were analyzed, and an algorithm of automated incident detection for freeway based on GPS was designed. A series of relative issues were also studied, including choice of sampling intervals, determination of detection thresholds, durative detection time and design of the alarm mechanism, etc. The validity and transplantability of the proposed AID algorithm were tested with field data and simulated data.

通过分析交通事件条件下车辆瞬时速度的变化特征，设计了1种基于GPS的高速公路交通事件自动检测算法，并对采样时间间隔的确定、检测阈值的确定、持续性检测时间的设定与报警机制设计等方法进行了研究，分别利用软件模拟数据和实测模拟数据对算法的有效性与可移植性进行了检验。

The selectively etching on germanium-on-glass structure can achieve visible light and infrared detection on the same chip with the unetched part for infrared detection and the etched part for visible light detection.

若在玻璃上覆锗的结构上做选择性的蚀刻，便可达到在同一晶片上在未蚀刻的部分来侦测红外光而蚀刻的部分侦测可见光的好处。

In the implement of the detection engine, the combination of anomaly detection technology and pattern match technology is used to redetection, which cans valid monish matching scope of object, and raise speed of detection.

通过协议分析模块，可以直接与相应的协议进行模式匹配，或者与建立相应协议的HMM模型进行异常检测，针对性较强，大大减少了匹配的次数。

The shaping method of noncircular part and the tool holder's radial motion characters in noncircular turning process are discussed in detail in the thesis.

论文详细研究了非圆零件的成型方法和加工过程中刀架的径向运动规律。

I have not really liked him,I do not like his this kind of disposition.

我没有真的喜欢他，我不喜欢他的这种性格。