查询词典 detection charge

Results By optimization test, capture antibody and detection antibody (from PVA detection kit, Agdia, USA) were finally diluted at 1:50 (4ug/mL) and 1:25 to be used in one step CBA, respectively. A total CBA reaction time of 2 h was needed. No cross reactivity with other plant virus similar to potato virus Y, lettuce mosaic virus and tomato ring spot virus were found. Detection sensitivity for positive control sample in one-step CBA was 4 folds higher than that in normal plate double antibodies sandwich enzyme-linked immunosorbent assay.

通过实验优化检测条件，最佳捕获抗体工作浓度为4μg/mL、最佳检测抗体工作浓度为1：25倍稀释、最佳反应时间为2h；与马铃薯Y病毒、莴苣花叶病毒、番茄环斑病毒等均未出现交叉反应；阳性样品经64倍稀释后依然可检出，检测灵敏度是传统微孔板ELISA的4倍。

Then the more advantaged detection technique is needed to be used in the pile detection. Computed Tomography is just such an advantaged technique, which has been widely and maturely used in iatrology, and in exploration of geophysics and scatheless detection in recent twenty years. It can reflect the inside structure of the inspected object precisely and gain the clear figure.

这就要求把更先进的检测方法应用到桩基检测中来，而层析成像技术就是一种非常先进的检测方法，它在医学中应用的非常成熟和广泛，最近二十年才应用到地球物理探测和无损检测中来的，它能精确反演被测物体的内部构造，得到清晰的图像。

Detection of wheel sideslip includes detection of sideslip between wheels and detection of sideslip between axles.

车轮侧滑检测包括轮间和轴间侧滑检测。

A ship target detection system, which based on the study of target detection, wake detection and feature extraction, has been developed.

在舰船目标检测、尾迹检测和特征提取的研究基础上，实现SAR图像舰船目标检测系统。

In order to extract the hierarchical and the narrative structure of news videos, the thesis puts forwards some low-level news video structure mining methods, including the shot detection method based on background tracking, the anchorperson shot detection method called AnchorClu and the news story detection method called SATS, which fuses multi-features.

为提取新闻视频的分层结构和叙事结构，提出了基于背景的镜头探测算法，基于聚类的播音员镜头探测方法AnchorClu，以及融合多特征的新闻故事单元探测方法SATS。

in order to improve the detection rate for anomaly state and reduce the false positive rate for normal state in the network anomaly detection, a novel method of network anomaly detection based on tsk fuzzy inference system was proposed.

在网络异常检测中，为了提高对异常状态的检测率，降低对正常状态的误判率，该文提出利用tsk模糊控制系统进行网络异常检测的新方法。

Unsupervised anomaly detection methods can detect the anomaly records in unlabled dataset. It can overcome the shortcoming of the traditional data mining methods, and automate the labeling and creating process of the intrusion detection model. It has become the useful tool of the intrusion detection.

以聚类为代表的无监督异常检测方法可以在无标记数据集上发现异常数据，克服了传统数据挖掘方法的缺陷，使标记数据集和入侵检测建模过程自动化，成为了入侵检测的有力工具。

Traditional data mining techologies used in data mining need to build intrusion detection model in labled data set. The process to lable data set is all by hands, and if the lables have some errors, the efficiency of the intrusion detection system will be affected. Unsupervised anomaly detection methods can detect the anomaly records in unlabled dataset.

传统的面向入侵检测的数据挖掘技术需要在大量的已标记数据集上建立入侵检测模型，数据集的标记过程需要以手工的方式进行，并且如果标记错误，将导致入侵检测系统检测效率的降低。

As a result, the DNA segment of predicted size, about 372 bp, appeared in the two kinds of vibrio, but didn't appear in the others. Therefore, a rapid detection kit for V. alginolyticus was developed successfully. It could provide a quick, exact diagnosis and detection method for the diseases in aquatic animals and human beings, and also for the aquatic food security caused by V.alginolyticus. 3. Furthermore, this detection kit was used to investigate the distribution of V.alginolyticus in seawater of living area, healthy aquicultural seawater ...

此外，我们利用此试剂盒对海南地区的生活区附近海水、水产动物养殖健康区海水和发病区海水进行了溶藻弧菌存在情况的调查，结果显示，生活区附近海水的溶藻弧菌数明显低于水产动物养殖健康区海水和发病区海水，后两者中又以发病区海水的溶藻弧菌数量最多；无论是在水产动物养殖健康区还是在水产动物养殖发病区，养殖场的进水处海水中溶藻弧菌的数量也明显低于养殖池水中的数量。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

The shaping method of noncircular part and the tool holder's radial motion characters in noncircular turning process are discussed in detail in the thesis.

论文详细研究了非圆零件的成型方法和加工过程中刀架的径向运动规律。

I have not really liked him,I do not like his this kind of disposition.

我没有真的喜欢他，我不喜欢他的这种性格。