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To elucidate the relationship of GSE expression and gametogenesis, mRNA (0.5μg each) from mouse testis and ovary on different postnatal days were analyzed by RT-PCR with specific primers for GSE cDNA. The GSE mRNA in the testis of mouse was first detected at Day 14 postpartum, when cells at mid-pachytene are likely to appear.

为了阐明GSE表达与配子发生的关系，从0～49日龄小鼠睾丸和卵巢中提取mRNA（每样品0.5μg，将其与特定的GSE cDNA引物进行RT-PCR反应，分析的结果发现，GSE mRNA在出生后第14天的小鼠睾丸中首先被检测到，这一时间正是中粗线期精母细胞很可能出现的时期。

They detected no gamma rays associated with the blast, confirming another prediction of the models.

研究人员没有探测到跟爆炸相关的伽马射线，证实了对此类星体的另一个预测。

The ORF2 gene is the most important protein-code gene for porcine circovirus, and is the main gene differing porcine circovirus typel from porcine circovirus type2 (PCV2). To construct a specific PCR detecting method, we designed a pair of primers to amplify a partial fragment of ORF2 gene from PCV2, referring to fifteen strains complete genome published in Genbank. The PCR product was about 0.67kb in size detected by gelose gel electrophoresis.

猪圆环病毒的开放阅读框2(ORF2)是其主要结构蛋白的编码基因，也是区分猪圆环病毒1型(PCV1)和猪圆环病毒2型(PCV2)的重要基因，本研究参照GenBank已发表的15株PCV2基因序列，设计合成一对引物，对PCV2的ORF2基因进行PCR扩增，产物经琼脂糖凝胶电泳，呈现一条大小约0.67kb的特异条带，经条件筛选，建立了猪病料中PCV2感染的PCR检测方法。

When he detected what Gemma loved another man, he was angry and jealous.

当他发现gemma喜欢另外一个男子时，他很生气和嫉妒。

MDR of pediatric genitourinary RMS could be detected in the level of protein and mRNA.

对于小儿泌尿生殖系横纹肌肉瘤可以在蛋白和mRNA水平检测出耐药基因。

Experiment 3: Affections of Ginger Extraction on Cardiac Systolic/Diastolic Function of Rabbits with Heart Failure and the Comparison Between Ginger Extraction and 4-Retro-decoction (Si-hi Tang,四逆汤) Method 50 pure-breed New-Zealand rabbits were randomly allocated into 5 groups: control group, low (0.028mL/kg), middle (0.056mL/kg) and high (0.112mL/kg) dosage Ginger group, and 4-Retro-decoction group. The heart failure model of rabbit was established by intravenous administration of 20g/L pentobarbital sodium (0.15 mL·kg〓·min〓) at a certain rate. Oral tube perfusion of Ginger extraction was administrated after the establishment of the model. Their hemodynamic changes were detected before and after the model establishment, and at 5, 10, 15, 20, 30, 45, 60, 90, 120, 150 minutes respectively after the ginger administration by a RM-6000 4-graph physiological monitor.

干姜提取物对兔急性心衰模型心脏功能及血流动力学的影响及其与四逆汤作用的比较方法以50只纯种新西兰兔为研究对象，随机分为低、中、高剂量干姜组，溶剂对照组和四逆汤对照组，共5组，以0.15mL·kg〓·min〓静脉恒速给予20g／L戊巴妥钠的方法建立兔急性心力衰竭模型，造模成功后，经口插管入胃灌服受试品，用RM—6000四道生理仪测定造模前、造模成功时及用药后5min、10min、15min、20min、30min、45min、60min、90min、120min、150min兔的心肌舒缩性能指标，观察各时段指标的变化情况。

Methods 40pure-breed New-Zealand rabbits were randomly divided into4groups:low（0.028mL/kg）,middle（0.056mL/kg）,high（0.112mL/kg）dosage ginger group and4-Retro-decoction group.The heart failure model of rabbit was established by intravenous administration of20g/L pentobarbital sodium at a certain rate.Oral tube perfusion of ginger extract and4-Retro-decoction were administrated after the establishment of the model.Their hemodynamic changes were detected before and after the model establishment,and at5,10,15,20,30,45,60,90,120,150minutes respectively after the ginger administration by a RM-6004-graph physiological monitor.

以40只纯种新西兰兔为研究对象，随机分为高、中、低剂量干姜组和四逆汤组共4组，用恒速滴注戊巴比妥钠的方法建立兔急性心力衰竭模型，造模成功后，经口插管入胃灌服受试品，用RM-600四道生理仪测定造模前、造模成功时及用药后5min、10min、15min、20min、30min、45min、60min、90min、120min、150min兔的心肌舒缩性能指标，观察各时段指标的变化情况。

Objective To investigate the difference between the effects of Ginger Extract and4-Retro-deˉcoctionon cardiac systolic/diastolic function of rabbit with heart failure.Methods 40pure-breed New-Zealand rabbits were randomly divided into4groups:low（0.028mL/kg）,middle（0.056mL/kg）,high（0.112mL/kg）dosage ginger group and4-Retro-decoction group.The heart failure model of rabbit was established by intravenous administration of20g/L pentobarbital sodium at a certain rate.Oral tube perfusion of ginger extract and4-Retro-decoction were administrated after the establishment of the model.Their hemodynamic changes were detected before and after the model establishment,and at5,10,15,20,30,45,60,90,120,150minutes respectively after the ginger administration by a RM-6004-graph physiological monitor.

目的 探讨干姜提取物和四逆汤对心衰兔心脏舒缩性能的作用异同方法以40只纯新西兰兔研究对象，随机分为高、中、低剂量干姜组和四逆汤组共4组，用恒速滴注戊巴比妥钠的方法建立兔急性心力衰竭模型，造模成功后，经口插管入胃灌服受试品，用RM-600四道生理仪测定造模、造模成功时及用药后5min、10min、15min、20min、30min、45min、60min、90min、120min、150min兔的心肌舒缩性能指标，观察各时段指标的变化情况。

Furthermore, older haplotypes were detected in the two groups, respectively. Possible refugia are inferred in western Hubei Province and SW China during the glacial period.

我们在最小生成树的两个组中推断出一些古老单倍型，并推测在冰期时湖北和我国的西南地区可能是该物种的避难所。

The report on using to modify,thermosetting resins and glass fibre reinforced plasticshave not been detected.

未见有NPMI改性热固性树脂和玻璃钢的相关文献和研究报导。

You need to lie low for a while.

你们得歇手一段时间

If you do, you may just be pleasantly surprised.

如果你开口问了，或许有意想不到的惊喜。