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And the repression of NO-induced cell death with Ppbi-1 overexpression in transgenic lines was apparent even at SNP concentrations up to 1mM.In addition work,we found that most of suspension cells treated with 0.5mM SNP can be stained by Sytox green,some PCD morphologic characteristics such as chromatin condensation and margination can be observed.DNA Ladder was also detected with these cells.As to the control and the cell treated with 0.5mMSNP+20μM Est,the above-mentioned characteristics can\'t be detected.

对0.5mM SNP和0.5mM SNP+20μM Est处理的飞廉转基因细胞培养三天后用特异性核染料sytox染色,0.5mMSNP单独处理的细胞大部分核被染色,而且细胞核出现了核凝聚、以及染色质边集等凋亡细胞的特征;提取细胞DNA电泳分析,观察到了DNA LADDER,这说明,该细胞可能已经发生凋亡,而Est诱导Ppbi-1基因表达的细胞均未出现这些凋亡特征。

objective to understand the status of sterilization in pedo-phore institution in jiangmen city; as to improve the quality of sterilization and to prevent the health of the children.methods the detected results of sterilization from 2003 to 2006 were analysis by retrospective method.results from 2003 to 2006 3079 samples were detected .the quam~ed rate of up to standard is 80.25%.the highest rate is 87.96% in 204, then 82.81% in 2003, 77.09% in 2005, 77.30% in 2006.there was no statistical significance for the qualified rate in air (83.38%), object surface (89.16%) and mess kit(88.28%); just as tile pubic- and personal- pedo-phore institution, the rate is 79.56% and 81.08%.

目的 了解江门市区托幼机构消毒工作状况,提高相关单位消毒质量,保护入托儿童身体健康。方法对本市区2003~2006年托幼机构监测结果进行回顾性分析。结果 2003~2006年间对本市区托幼机构采样 3 079件,总合格率为80.25%。2004年监测合格率最高为87.96%,其次为2003年82.81%,2005年、2006年合格率分别为77.09%、77.30%。不同监测项目中空气、物件表面、餐饮具合格率分别为83.38%、89.16%、88.28%。工作人员手合格率较低为52.53%,差异有统计学意义。

At the same concentrations of saccharose , the lactic acid accumulation amounts of the treatments with inoculation are lower than those of the controls , and in controls methylamine is detected because of the decomposition of protein by pathogenic bacteria , but in treatments of inoculation they are not detected .

相同蔗糖浓度下,接种处理的丁酸积累量均低于对照,不接种对照处理中均检测到了腐败菌分解蛋白质产生的甲胺,而接种的则没有。

The antibiotic resistance of staphylococcus aureus was detected by K-B method. Of 33 staphylococcus aureus stains isolated from food, 28 were resistant to penicillium and ampicillin, the resistance rate was as high as 84.8%.21 stains were resistant to carbenicillin, the rate was 63.6%.5 stains were detected resistant to methicillin, which took up to 15.2%. The stains also were resistant to cephalosporin antibiotics, aminoglycoside, microcline antibiotic and tetracyclines in different levels, all strains were sensitive to vancomycin.

结果 33株食品中分离的金黄色葡萄球菌,有28株对青霉素和氨苄西林耐药,耐药率高达84.8%;21株对卡比西林耐药,耐药率达63.6%;检出了5株耐甲氧西林的菌株,耐药率为15.2%;对其他头孢类抗生素、氨基糖苷类、大环内脂类、四环素类等都出现出不同程度的耐药;所有菌株均对万古霉素敏感。

The pathogenic bacteria of AV was primarily Gram-positive aerobic bacteria,with the detection rate of 80% including Staphylococci aueas,Enterococcus faecalis and E.coli;low rate of B.streptococcic was detected.The pathogenic bacteria of BV was primarily Gram-negative anaerobic bacteria,while the rate of Gardnerella was detected as less than 40%.There were 8 cases with of AV concurrent infection in 48 cases of BV infection,and 6 cases with BV concurrent infection in 31 cases of AV infection.Conclusion Microphyte profile analysis indicated that patients infected by AV were susceptible to anaerobic bacteria such as Gard...

正常女性阴道中以乳酸杆菌为主,产H2O2乳酸杆菌的检出率89%,AV的致病菌主要是革兰阳性率需氧菌、检出率可达80%,其中金黄色葡萄球菌,粪肠球菌和埃希氏大肠菌较为常见,B族链球菌的检出率较低;BV的致病菌主要是厌氧菌,以革兰阴性厌氧菌的检出率最高,加德纳菌的检出率不足40%。48例BV患者中有8例合并AV感染,31例AV患者中有6例合并BV感染。

The I/D polymorphism of ACE was detected by polymerase chain reaction, and the CMA/B poly- morphism of Chymase gene was detected using PCR-restriction fragment length polymorphism.

运用聚合酶链反应检测 ACE基因的I/D多态性,运用限制性片段长度多态性技术检测Chymase基因的CMA/B多态性。

Methods RF was measured by rate nephelometry method, anti-CCP antibody was detected by ELISA, and anti-Sa antibody was detected by immunoblotting testing in 72 patients with RA, 115 patients with autoimmune diseases, and 102 healthy controls. The consistency of their clinical significance and other indexes were intercompared and analyzed.

对72例RA患者组,115例自身免疫性疾病组和102例正常对照组人群血清进行同时测定RF、抗CCP抗体和抗Sa抗体,并对三者之间检测的一致性以及与其他临床指标的关系进行了分析。

Noll. From the irradiance distribution in two defocal planes, one in front of focal plane and the other behind, with a given detector array the elements of response matrix for each normalized term of Zernike polynomials can be obtained. For an incoming atmospheric disturbed random wavefront, with its irradiance distribution detected in two defocal planes and the response matrix, it is easy to solve the corresponding Zernike term coefficients of incoming wavefront and reconstruct the detected one.

Noll建议的Zernike多项式表征经大气扰动的入瞳处的光学波前畸变;求出由两个离焦面上的光强分布决定的归一化Zernike项在特定形状的探测器上的响应矩阵;由响应矩阵和入瞳处的波前在两个离焦面上的光强分布,求出其Zernike项的系数,从而实现波前的探测和重构。

Results: No protein peak was detected in thirty urine samples of the control group, hut protein peaks were detected in seventy urine samples of the case group with the exception of two and among them 16 were selective proteinuria, 50 were nonselective proteinuria and 2 were overflow proteinuria.

用微流控芯片电泳对30例尿蛋白定性试验为阴性的对照组和70例尿蛋白定性在++以上的病例组进行检测,以白球蛋白的峰面积比值判断蛋白尿的选择性,并与美国Helena琼脂糖凝胶电泳结果比较。

Results:No protein peak was detected in thirty urine samples of the control gruop, but protein peaks were detected in seventy urine samples of the case group with the exception of two and among them 16 were selective proteinuria, 50 were nonselective proteinuria and 2 were overflow proteinuria.

用微流控芯片电泳对30例尿蛋白定性试验为阴性的对照组和70例尿蛋白定性在++以上的病例组进行检测,以白球蛋白的峰面积比值判断蛋白尿的选择性,并与美国Helena琼脂糖凝胶电泳结果比较。

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