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The function of EM65-2 on the RL95-2 was detected by in vivo and in vitro test. We established the protocol of making the human RL95-2 endometrial carcinoma xenografts in the ovariectomized nude mice at first. Secondly we detect the function of EM65-2 on the subcutaneous enografts with or without benzoate estradiol and estrone. The function was evaluated by the change of tumor volume and the weight of the tumor and the uterine at the end of the test.

VEGF的调控分也分为体内和体外两部分进行,体内部分利用前面实验中获得的不同条件下的裸鼠的皮下移植瘤和小鼠的子宫,通过免疫组化方法检测不同雌激素和抗雌激素的条件下的VEGF的表达情况;体外部分,首先建立RL95-2细胞的无血清培养方法,然后建立无雌激素正常pH、无雌激素低pH的培养液和雌二醇浓度为10nM的正常pH等三种条件培养液,于培养的不同时间分离上清液,通过Elisa法检测上清中VEGF的变化。

Long filamentous and rhabdoviruses were first found in garlic. The leaves were detected by DAS-ELISA,and the result showed that masaic garlic contained LYSV and PVX,etiolation garlic contained OYDV and GCLV,leaf roll garlic contained GCLV、LYSV and OYDV.

发病寄主叶片的DAS-ELISA检测结果:花叶症状的大蒜样品中含有LYSV和PVX,黄化症状的大蒜样品中含有OYDV和GCLV,卷叶症状的大蒜样品中含有GCLV、LYSV和OYDV。

Results: the yield rate of the collected volatile oil of artemisia indica and artemisia feddei was 0.32%, 0.29%, respectively. the main constituents of them were as follows: eucalyptol, camphor, borneol, terpineol, cubebene, caryophyllene, caryophyllene oxide, germacrene d, etc, which was similar to the constituents of volatile oil from artemisia argyi. but the contents of volatile oil were different in different medicinal materials. longiverbenone with high content in artemisia feddei was can not be detected in artemisia indica and artemisia argyi.

结果:广西产五月艾与细叶艾挥发油的得率分别为0.32%、0.29%;五月艾、细叶艾和艾叶挥发油中均含有桉油精、樟脑、龙脑、松油醇、荜澄茄油烯、石竹烯及其氧化物、大根香叶烯d等药效成分,但各成分在不同药材中的含量存在一定差别;此外,细叶艾挥发油中含量较高的长叶马鞭烯酮在五月艾和艾叶挥发油中均未检出。

At approximately 05.30 on Saturday 24 May, as the German squadron was about to leave the Denmark Strait, Prinz Eugen's hydrophones detected the presence of two additional ships some distance to port.

5月24日,星期六,早5点30分,当德国舰队正要离开丹麦海峡的时候,"欧根亲王"上的声纳系统探测到有另外两艘舰船在他们的左翼。

Results: miR27a was identified as the most stable reference gene by geNorm and Normfinder software. Among the 52 microRNAs detected by real time PCR array, miR-33 and miR-19a were significantly down-regulated, whereas miR-130 were significantly up-regulated in the hippocampus of Ts65 Dn mice as compared with the euploid control mice. The expression of miR-802, which was the trisomy chromosome 16 derived microRNAs, was very low in hippocampus with no difference between the two groups.

结果:geNorm和Normfinder两种方法均选定miR-27a为最稳定的内参照基因,共检测了52种microRNAs,其中miR-33和miR-19a在Ts65Dn小鼠海马组织中表达显著下调,miR-130在Ts65Dn小鼠海马组织中表达显著上调,小鼠16号染色体三体区段包含的miR-802在Ts65Dn小鼠和对照二倍体小鼠海马组织中表达量均低,差异没有统计学意义。

In about 80%(32/40) of protan and deutan the changing of exon 5 can be detected.

这些指标在基因诊断中有一定的应用价值。

Methods:The mutations of exon 5,6,7 and 8 of p53 gene in 135 patient with colcorectal cancer were detected by PCR-SSCP.

应用 PCR-SSCP 技术检测 135 例大肠癌患者 p53 基因第5、6、7、8外显子的突变。

The changes of microorganisms' number in different kind of Red Soil from CAS Ecological Experiment Station were detected.

跟踪检测中科院红壤生态站三种不同土壤中微生物数量在一年中的变化。

The stochastic exponential growth model and birth-death process model were established to analyze the extinction risk and population viability with detected data of wild Pere David's deer populations.

利用野放麋鹿Elaphurus davidianus种群检测数据建立了随机指数增长模型与生死过程模型,并对种群的未来状态、灭绝风险及种群生存力进行了分析。

The results showed that the SCE value detected in the exposer in first year approximated to that of the second year, and both were significantly higher than that of normal controls.

本文在两年内观察了长期接触化学致癌物的肿瘤研究室工作人员以下简称接触者

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The objective is to subjugate and discourage the people, because that allows the elite to continue to rule unopposed.

其目的是压制和打击人民的积极性,因为这可以让实权派继续统治不会沦为反对派。

GOD,this is the second time you vanquished me!

天啊,这是第二次你打败了我!

So a kind of strong antagonism permeated in the relations of two countries.

所以两国这一时期的政治关系始终弥漫着一种强烈的对抗情绪。