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The difference of enumeration data was detected with )

计数资料差异性测定采用x2检验。

This study that observed the expression and distribution of 4 apoptotic factors, relation apoptotic factor with apoptosis, changes of T and B subgroup, and changes of molecular pathology in 14 cows suffering from enzootic bovine leucosis from several resides was detected by histochemistry, TUNEL technology, immune-fluorescent flow cytometry and so on. The aim of this experiment supplies theoretic basis for preventive and therapy of EBL, and consult dates for T lymphoid cell leucosis of human being .

本研究检查并剖检了14例临床与实验室诊断为EBL的病牛,观察分析了肿瘤发生的情况和特征,采用免疫组织化学ABC法、原位末端标记TUNEL技术和免疫荧光流式细胞检测法等技术,从EBL肿瘤细胞凋亡特性入手,围绕4种凋亡因子和几种免疫相关因子与EBL肿瘤发生的关系及其在诊治方面的应用进行了首次有价值的探索,其目的在于为防治EBL提供科学的理论依据,同时,也将为人类T细胞白血病Ⅰ。

It is some uncertain chlorinated compounds not pentachloro ethane and/or hexachloro ethane however that can be detected in most EOM from sedimentary rocks, indicating that the interaction of copper with chloroform can be restrained by EOM and/or they may be transformed into other chlorinated compounds.

铜对氯仿的催化会影响氯仿沥青的定量和组成。

Vascular invasion was also detected by hematoxylin and eosin stains.

应用免疫组化方法检测标本中血管内皮生长因子的表达及微血管计数,HE染色显示脉管浸润。

In blastula embryos and 50% epiboly embryos, the zygotic pou2 transcript is also distributed in every blastomere cell. At the stage of 90% epiboly, expression of pou2 converges to the dorsal midline of embryos and locates in two waves of lateral neural plate and two transverse stripes in the brain region. In embryos at 100% epiboly, pou2 transcript is restricted in the neural plate and areas of presumptive midbrain amid himdbrain. From somite stage, gibel carp pou2 can not be detected any more, which is consistent with the RT-PCR result.

在高囊胚期和50%下包期,高度表达的合子转录本仍在所有的胚盘细胞中,但至90%下包期时,pou2的表达向胚胎背部的正中线汇聚,集中在神经板的两侧区域和脑部的两条横向条带在100%下包期时,pou2的表达集中在神经板的中间区域以及预期形成的中后脑区域至体节期时,转录本消失,这与RT-PCR结果高度一致。

Temporal and spatial expression pattern of gibel carp pou2 during embryogenesis was investigated by RT-PCR and whole-mount in situ hybridization. RT-PCR result indicates that there is maternal transcript of gibel carp pou2 in early embryos and the zygotic pou2 transcript is abundantly expressed from blastula stage. Large amount of pou2 transcript is detected at both 50% and 90% epiboly stages. At 100% epiboly stage, however, the pou2 transcript reduces rapidly and disappears at the later embryogenesis. Whole-mount in situ hybridization shows that the maternal transcript ubiquitously exists in all blastomere cells.

我们用RT-PCR和整体原位杂交的方法研究了银鲫pou2基因在胚胎发育过程中的时空表达图式RT-PCR结果显示,银鲫pou2基因有母源转录本,其合子基因在高囊胚期强烈表达,在50%下包期和90%下包期也有高量的转录本,但在100%下包期表达量急剧降低,至体节期时已经完全检测不到其转录本胚胎整体原位杂交结果显示其母源转录本在所有的胚盘细胞中。

The ultrastructural localization of the immunoglobulin and complements in diseased skin of several sub-epidermal bullous diseases, including 1 case of Bullous Pemphigoid , 3 cases of Epidermolysis Bullous Acquisita , 1 case of Bullous System Lupus Erythematosus and 2 cases of Linear IgA Bullous Dermatoses , was detected by postembedding immunogold electronmicroscopic technique.

利用直接免疫金标电镜技术同时检测几种表皮下大疱病:大疱性类天疱疮1例、获得性大疱性表皮松解症3例、大疱性SLE1例、线性IgA大疱性皮肤病2例皮损组织中免疫球蛋白和补体〓,比较它们沉积部位和形态的异同,以探讨该技术在鉴别不同表皮下大疱病中的意义。

Preadipocytes from epiploon of newborn calf were cultured by monolayer adherent method, and total RNA of the preadipocytes was extracted on day 4,6,8,10,12,14 and 16 post-culture, respec-tively. The abundance of ADPN mRNA and HSL mRNA was detected by real-time fluorescent quanti-tative RT-PCR during differentiation period from preadipocyte to adipocyte.

选取犊牛大网膜脂肪组织,进行牛前脂肪细胞的单层贴壁培养,在细胞接种后第4、6、8、10、12、14、16d分别提取细胞总RNA,采用实时荧光定量RT-PCR方法,检测了体外培养的犊牛前脂肪细胞不同时期ADPN基因mRNA和HSL基因mRNA的表达情况。

The process of extraction of procyanidin from sorghum episperm was studied, the extract was detected for DPPH activity; and the extraction process was applied to practical extraction of procyanidin from six kinds of sorghum episperm.

对高粱种皮中原花青素的提取工艺进行研究,对提取物进行了DPPH活性检测;并将提取工艺应用于6种高粱种皮的原花青素提取实践。

All QTLs detected in this study were affected by epistasis of additivexadditive and interactions of QTLsxE.

本研究所定位到的QTLs中都存在加×加上位性效应牙QTL与环境的互作效应。

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The objective is to subjugate and discourage the people, because that allows the elite to continue to rule unopposed.

其目的是压制和打击人民的积极性,因为这可以让实权派继续统治不会沦为反对派。

GOD,this is the second time you vanquished me!

天啊,这是第二次你打败了我!

So a kind of strong antagonism permeated in the relations of two countries.

所以两国这一时期的政治关系始终弥漫着一种强烈的对抗情绪。