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Methods: The telomerase activity in breast cancer cells and MRC-5 cell line were detected semi-quantitively by TRAP-ELISA assay. Virus proliferation as- say and cell viability assay were used to evaluate the proliferation and cytolysis selectivity of CNHK300, and the results were compared with those of wtAd5. The breast cancer cells were treated with CNHK300 combining paclitaxel to evaluate the killing effect of the combined regimen.

用TRAP-ELISA方法检测乳腺癌细胞株(MDA-MB-453、SK-BR-3)和正常成纤维细胞株MRC-5的端粒酶活性；进行病毒增殖实验和细胞生长抑制实验，并与野生型腺病毒wtAd5进行对比，验证CNHK300选择性复制和选择性杀伤能力；用细胞生长抑制实验进一步考察CNHK300与化疗药物紫杉醇联合应用对乳腺癌细胞的杀伤作用；用Western blot检测腺病毒E1A在细胞中的表达。

Flow cytometry showed that the proliferation of splenocytes and cytolysis to tumor cells detected in early phase of tumor development were enhanced in the presence of Tim-3, and this anti-tumor effect was further improved by the combined use of Tim-3 with 4-1 BBL.

膜型Tim-3可在免疫启动阶段作为正向免疫调节因子增强抗肿瘤免疫应答，并可与4-1 BBL协同产生更强的抑瘤效应。

488Anti-rat CD106 and Bis-Benzimide staining to calculate cytoplast purity. Mitochondrial membrane potential was detected by flow cytometry following labeling with Rhodamine123. RESULTS: The purity of cytoplasts was up to 95%.

488anti-rat CD106及Bis- Benzimide染色进行形态学观察，计算胞质体纯度；应用Rhodamine123标记后用流式细胞仪检测胞质体中线粒体膜势能。

It shows in the test of simulation sample that the ratio of interference inhibition is higher than 5000, and the minimum detected discharge magnitude is 0.06pC. Again, a method of polarity discrimination and high interference inhibition for the dual-input and single-output damped oscillation signal is proposed firstly.

在脉冲鉴别法的基础上，通过详细分析脉冲鉴别的原理和双端输入、单端输出信号的极性，首次提出了对双端输入、单端输出衰减振荡的局部放电信号进行极性鉴别的方法和极大干扰信号抑制方法。

Results 7 lung cancer patients were detected three marker VOCs in their breath: Benzene,Propyl benzene and Decane.

使用TEDLAR气袋收集此8例患者的呼吸气体，然后使用固相微萃取－气相色谱对呼吸气体进行分析，根据其出峰时间的不同对VOCs定性，测得患肺癌的患者的呼吸气体中含有苯、丙基苯和癸烷三种特征性气体，而炎症患者的呼吸气体中则没有出现这样的特征性气体。

Compared the VOCs in lung cancer patients and normal people and metabolic product of lung cancer cells to analyze the correlation between lung cancer and its marker VOCs.Results 7 lung cancer patients were detected three marker VOCs in their breath: Benzene,Propyl benzene and Decane.

在肺癌患者的呼吸气体中所含的特征性有机成分的检测方面［1～3］，已经有部分科研人员做了尝试，也有培养肺癌组织细胞并探测其代谢气体成分的研究［4］，期望能建立通过检测患者的呼吸气体即能够初筛肺癌患者的简便方法。

The decimal point on the seconds digit is turned on when sync is detected and turned off when sync is lost.

秒小数点的数字时打开同步检测并关闭时，同步丢失。

The photocatalytic properties of these samples were detected by decoloration rate of methyl orange solution and COD.

通过测试甲基橙溶液的脱色率和COD去除率对复合光催化剂的光催化活性进行了评价。

The AHLs were detected in three Pseudomonas strains by the AHLs biosensor and degraded by the AHLs degrader strain.

方法］利用AHLs检测菌对3株假单胞菌AHLs产生情况进行检测，并通过AHLs降解菌株对所产生的AHLs进行降解。

Basing on the model, we observed the vomiting times and delitescence, detected gastrointestinal hormones and neurotransmitters by radioimmunoassay and by HPLC-ED.

在此模型的基础上，观察家鸽呕吐次数和潜伏期，用放免试剂盒检测胃肠激素(EGF、GAS、VIP)；用高效液相色谱-电化学法检测神经递质（5-HT、DA）的含量。

The objective is to subjugate and discourage the people, because that allows the elite to continue to rule unopposed.

其目的是压制和打击人民的积极性，因为这可以让实权派继续统治不会沦为反对派。

GOD,this is the second time you vanquished me!

天啊，这是第二次你打败了我！