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The level of serum amylase, the pulmonary dry/wet weight ratio and histological changes were measured at different time points in each group. The expression of pulmonary CINC protein and mRNA were detected by immunohistochemistry and semi-quantitative reverse transcriptase polymerase chain reaction, respectively.

分别检测各组不同时间点血清淀粉酶、肺干湿重比和肺组织病理学改变，用免疫组织化学法和半定量逆转录聚合酶链反应法检测肺组织中CINC蛋白和CINC mRNA表达的变化情况。

The phase of optical wave can be recovered by the intensity transport equation under the paraxial approximation For the optical system with diffraction limit, it is difficult to get the boundary radical slope of phase and sample in the circular domain boundary when using the intensity transport equation to recover the phase, consequently, we reviewed an algorithm mentioned in the reference ［4］, namely, the equation, computation domain and boundary condition are changed, then the phase is reconstructed by the multigrid method and corrected at last Moreover, the experiment system is set to testify the algorithm, and the recovered phase from the intensity data detected by CCD is compared with the result from phase retrieval algorithm, it is found that the phase reconstruction error can reach 017 λ in the case of non-uniform intensity distribution, as a result, the method is suitable for the phase recovery with low wavefront sensing accuracy.

在傍轴近似条件下，可以利用光强传播方程对畸变波前进行相位恢复。对于衍射受限的光学系统，很难获得相位的边界径向斜率值作为边界条件，此外，要获得精确的圆域边界采样值也并非易事。为了克服上述困难，进一步研究了相位恢复改进方法，即改变了方程的表示形式、计算区域和边界条件，并用多重网格法进行求解获得重构相位，最后再将其修正。为了验证算法的精确性，搭建了实验系统对算法进行实验验证，将由CCD探测的光强分布进行计算得到的相位分布与相位恢复算法的结果进行比较，证明在光强分布非均匀的情况下，该方法恢复的相位均方根误差能够达到017 λ，可以适用于波前传感精度要求不是很高的相位恢复。标签自适应光学相位恢复相位修正光强传播方程 adaptive optics phase retrieval phase correction intensity transport equation

Methods: Parallel-plate-flow-chamber was used to assay the influence of shear stress on endothelial cells morphology and cytoskeleton and apoptosis and NO secretion. endothelial cells morphology was observed under microscopy, cytoskeleton was showed by Coomassie brilliant blue staining, fluorescence stain, and transmission electron microscopy, apoptosis was detected by FCM, NO of circulating culture medium was assayed by NO kit.

细胞形态显微镜下直接观察；细胞骨架通过考马斯亮蓝染色、荧光标记和透射电镜进行观察；细胞凋亡通过流式细胞仪检测进行分析，一氧化氮通过流动循环培养基的收集后应用检测试剂盒进行检测分析。

In this paper,the growth,the fermentation conditions,the production of monacolin K and so on were studied;and the monacolin K ,the citrinin and the flavone was detected and analised.

本论文主要是研究了红曲霉在荞麦介质上的生长、发酵条件、产生莫那可林K的情况，并对莫那可林K、桔霉素、黄酮作了分析检测。

Methods Three experimental groups of ROS17/2.8 cells were cultured in rotating clinostat for 24 h, 48 h and 72 h respectively, 1 h before the end of culture, BMP-2 (500 ng/ml) was added into the medium, and the expression of total ERK1/2 and phosphated-ERK1/2 (p-ERK1/2) were detected by Western Blotting method at th...

方法在地面1G和回转器模拟失重条件下培养ROS17/2·8细胞，培养时间分别为24h、48h和72h。

It was he who first detected the small variations in hundreds of stars closely packed into the globular clusters.

正是他首先发现了星团中密密麻麻的千百颗星球中的细微变化。

If this type of clot is detected in time, it can normally be removed by a straightforward operation with effective results.

如果有这种类型的血块是在检测时间，它通常可消除一个简单的运作与有效的结果。

Such analysis shows that:(1) Some OH masers are confirmed of their radiative pumping mechanism while some other OH masers might be pumped by other mechanisms, and for another set of OH masers whose radiative pumping lines which is expected by our estimation based on modeling work are not detected, which may be due to other reasons such as clumpy OH shell and limb filling emission effect.

这些分析结果显示：(1)除了一些OH／IR源的OH 1612MHz脉泽可能确实是由红外辐射抽运的外，另有一些OH／IR源可能是由其它抽运机制抽运的，还有一些OH／IR源，可能是由于诸如OH脉泽壳层分布不均匀或OH脉泽壳层填充发射等原因，其光谱中的这两条红外吸收线按照模型估计本应该被探测到而实际上却没有探测到。

Methods Expressions of Ph chromosome and bcrabl mRNA of 437 CML patients were detected at the same time.

目的 探讨Ph染色体和bcrabl mRNA在慢性粒细胞白血病诊断、分期、治疗及微小残留病变监测中的意义。

Results Among 70 strains of Staphylococcus spp., 56 strains were positive Staphylococcus coagulase gene with PCR; the same 56 strains were also positive with latex agglutination test; 54 of which were positive with tube test method, 2 strains were false negative; strong positive were detected with slide method, all these 56 strains were identified as Staphylococcus aureus by VITEK-Ⅱ automatic analyzer.

结果 70株葡萄球菌属细菌中，PCR法检测葡萄球菌凝固酶基因56株为阳性；Slidex Staph Plus与PCR法相对应的56株为阳性；试管法检测与前2种方法相对应的54株为阳性，2株为假阴性；玻片法为强阳性；VITEK-Ⅱ全自动分析仪鉴定56株为金黄色葡萄球菌

The objective is to subjugate and discourage the people, because that allows the elite to continue to rule unopposed.

其目的是压制和打击人民的积极性，因为这可以让实权派继续统治不会沦为反对派。

GOD,this is the second time you vanquished me!

天啊，这是第二次你打败了我！