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The primary subject of this paper is to (1) The negative-selection algorithm that can be detected abnormity is improved by the mutation mechanism of genetic algorithm.

本文所做的主要工作如下(1)借助遗传算法中变异的机制,对能检测出异常变化的阴性选择算法作了新改进。

Cytokines in abomasum of goats immunized with rHco-GAL-f/m were detected in this study.

检测重组雌、雄捻转血矛线虫半乳糖结合凝集素(rHco-GAL-f/m)免疫山羊皱胃转录细胞因子。

Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%,0 and 38% respectively in sorted GPA positive cells of 3 samples by FISH,and the results of GPA+ cells of the 3 cases by PCR were positive,negative and positive respectively,which were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16~24周的孕妇血中分离出NRBCs,并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果,3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%;其PCR结果,分别为阳性、阴性、阳性,并在引产后得到性别证实。

Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%0 and 38% respectively in sorted GPA positive cells of 3 samples by FISHand the results of GPA+ cells of the 3 cases by PCR were positivenegative and positive respectivelywhich were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16~24周的孕妇血中分离出NRBCs,并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果,3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%;其PCR结果,分别为阳性、阴性、阳性,并在引产后得到性别证明。

Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200232,ChinaAbstract: Nine samples from swine abortus were detected by RT-PCR with a pair of primers specific to NS1 gene of Japanese encephalitis virus.

采用反转录聚合酶链式反应方法检测猪流产胎儿病料中的乙型脑炎病毒,并对阳性病料做病毒分离与纯化。

The omp17.3 gene of Brucella abortus was amplified by PCR and then the amplicon was cloned into the eukaryotic expression plasmid pcDNA3.1 to construct a recombinant plasmid pcDNA3.1-omp17.3. Then the recombinant plasmid pcDNA3.1-omp17.3 was transfected into COS7 cells, and the expressed OMP 17.3 was detected by Western-blotting.

采用PCR方法扩增了布鲁氏菌17.3ku外膜蛋白编码基因,并将该基因克隆至真核表达载体pcDNA3.1中,成功构建了真核表达质粒pcDNA3.1-omp17.3.pcDNA3.1-omp17.3转染COS-7细胞后,通过Western-blotting检测到了17.3ku蛋白的瞬时表达。

Your wage will be lower than most of the other people, if your absenteeism is detected by the manager.

如果你旷工被经理发现,你这个月的薪水会比大部分人都低。

Methods patients in 4 different groups including pulmonary acariasis group (n=35), scabies group (n=35), demodex group (n=38) and control group (n=30) were detected by the two methods, and the results were compared each other.

应用SPA -ELISA和Map-IFAT分别对肺螨病组(35例)、疮疮组(35例)、蠕形螨组(38例)、对照组(30例)进行血清抗体检测并对比分析。

Materials and 64 dust acarid allergic rhinitis outpatients were randomly divided into 2 groups, 31 patients in the control group were treated with general immunotherapy, 33 patients in the trial group were treated with NHD, the serum level of IL-4, IL-5 was detected by ELISA.

常年性变应性鼻炎患者64例,随机分为常规免疫治疗组31例,标准化尘满变应原疫苗治疗组33例,比较两组患者在接受免疫治疗后的疗效及血清IL-4、IL-5水平。

But it can be detected if the water happens to have been taken from an accumulator which has been used for some time.

但如果从使用了一段时间的蓄电池中取水的话,便可以检测出来。

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