查询词典 detected

Alter intragastric administration of Ecr to rats, the effect of Ecr on the changes of gastric motility was detected. At the same time, the changes of frequency of electro-physiological activity, as well as expression of c-Fos in nucleus tractus solitari and dorsal-nuclei of the central bulb were also observed. The mechanism of Ecr on gastric motility were also detected by observing the activation of the NTS and DMV.

在前期工作的基础上，采用灌胃给药方法，给大鼠以最有效剂量的萝卜提取物按60mg/kg灌胃后，经胃电记录，观察其对胃运动的影响，并对其对中枢支配胃运动的迷走复合体(dorsal vagal complex， DVC)中神经元放电频率的变化以及c-Fos表达的情况为标志，观察上述两个核团中神经元被激活状况，来探讨萝卜提取物促胃动力的作用机制。

The expression of p53 was detected in thymocytes, splenic cells. p53 gene was not detected in liver.

脾脏、胸腺细胞中户p53蛋白表达阳性，肝脏细胞中p53蛋白表达阴性。

A portion of network detected by only one sniffer for all traffic passing through it is detected while all sniffers exchange detections for the optimization.

在抗体的进化过程中，提出了较好地评价抗体适应度的评估函数，为优化抗体的遗传算法的实现打下了基础。

The summary results are below:1. GUS expression under the driving of the BjCHI1 promoter (-1060/+17) was essentially undetectable in the young seedlings under normal growth conditions. GUS activity was first detected in the stigma of young flowers, peaked in the young siliques, and decreased when the siliques became older. No GUS expression was found in the mature siliques, seeds or root.2. The BjCHI1 promoter (-1060/+17) was inducible by NaCl, PEG, wounding and MeJA treatments. High levels of GUS expression were detected in the transgenic tobacco and Arabidopsis plants after wounding, NaCl, PEG, and MeJA treatment, indicating that the BjCHI1 promoter responses to both biotic and abiotic stresses.3. RT-PCR analysis confirmed that the expression of the BjCHI1 gene in B. juncea was inducible by PEG and NaCl.4. The transcription start site was determined by 5′-RACE, and was located at the 17th nucleotide upstream of the translation initiation codon of the BjCHI1 gene.5. A -805/+17 promoter fragment was enough to response to wounding and MeJA induction, which was proved in transgenic tobacco and Arabidopsis plants. The 397 bp region between -805 and -409 of the BjCHI1 promoter contains a cis-acting element that is essential for the wounding and MeJA inducibility.6. The -695/-620 region was necessary but not sufficient to confer MeJA-responsive expression. A T/G-box locates in -353 play an important role in the expression of the BjCHI1 gene in response to MeJA treatment. The 76 bp region is coupled with the T/G-box to confer full MeJA-inducible transcription of the BjCHI1 gene.

主要结果如下：1、利用转基因拟南芥植株分析表明，正常生长条件下，BjCHI1启动子(-1060/+17)驱动GUS基因主要在花柱中表达，幼嫩的荚也有表达，并随着果荚的成熟而减弱，成熟的果荚、种子和根没有显示GUS活性。2、BjCHI1启动子(-1060/+17)能驱动GUS基因在转基因烟草和拟南芥中响应伤害的诱导，转基因拟南芥的分析还证明BjCHI1启动子也受MeJA、NaCl和PEG的诱导，证明BjCHI1启动子是一个伤害、MeJA、NaCl和PEG等生物和非生物因素诱导启动子。3、RT-PCR进一步证明芥菜中BjCHI1基因也受NaCl和PEG的诱导表达。4、5′-RACE法鉴定了BjCHI1启动子的转录起始位点，位于翻译起始位点ATG上游第17个碱基A.5、转基因烟草和拟南芥分析证明，-805/+17的启动子片段足以响应伤害和MeJA的诱导，-805和-409之间397 bp的启动子片段含有对伤害和MeJA诱导必要的元件。6、本明烟叶片瞬时表达系统分析证明，一段76 bp的序列(-695/-620)对BjCHI1启动子响应MeJA的诱导是必要的，但不足以响应MeJA的诱导，位于-353的T/G-box也参与MeJA的诱导。76 bp的序列(-695/-620)与T/G-box协同起作用，赋予BjCHI1启动子MeJA诱导性。

Methods The serotyping, hybridization and RAPD typing methods were used. Seven strains of GBS were detected in 2 pairs of mother-baby, which included 2 strains from the 2 mothers and 5 strains from their babies with early and late-onset infections. Eighty-eight strains of GBS were detected in vaginas of pregnant womens.

采用血清学、核酸杂交和PARD方法对从2例诊断为早发型GBS肺炎和晚发型GBS脑膜炎患儿体内分离的5株GBS和其母阴道分离的2株GBS菌株作母婴配对分析，对88株孕妇生殖道携带GBS进行RAPD分型研究。

Methods: A randomized, single blind controlled clinical design was used to accomplish the series clinical trials included 123 cases. The troop of gatifloxation injection with sodium chloride included 20 cases in gatifloxation group and 23 cases in ciprofloxation group; The troop of gatifloxation injection with glucose included 21 cases in gatifloxation group and 19 cases in levofloxacin group; The troop of gatifloxation pivoxil included 19 cases in gatifloxation group and 21 cases in ciprofloxation group. The antibacterial activity of clinical isolates were detected by scrips which contain gatifloxation(5ug/L), levofloxacin (5ug/L), ciprofloxation (5ug/L), spafloxation(5ug/L), cefotaxime(30ug/L), penicillin(10U/L) respectively. The MICs of sixkinds of antibiotics were detected by 2-fold agar dilution method. Result: The common data of patients in the two groups of 3 troops were similiar.

采用区组分层均衡随机单盲试验设计，分别完成甲磺酸加替沙星氯化钠注射液组试验药20例及对照药23例、甲磺酸加替沙星葡萄糖注射液组试验药21例及对照药19例、甲磺酸加替沙星片剂组试验药19例及对照药21例共123例感染患者的临床试验；用K-B法测定临床分离致病菌对加替沙星(5ug／L)、左氧氟沙星(5ug／L)、环丙沙星(5ug/L)、司帕沙星(5ug/L)、头孢噻肟(30ug／L)、青霉素(10U／L)纸片的敏感性，再用按美国国家实验室标准委员会(National Committee for Clinical Laboratory Standards，NCCLS)推荐的琼脂平皿对倍稀释法测定临床分离菌株对加替沙星、左氧氟沙星、环丙沙星、司帕沙星、头孢噻肟、青霉素的最低抑菌浓度(Minimum Inhibitory Concentration，MIC)以了解其体外抗菌活性。

The biological activity of IFN-γwas detected in the supernatant of the cultured 293 cells following rAds' infection, and it could be blocked by the antibody for IFNγ. The B16F10 cells infected with the purified rad (RSV/IFN-01) didn't show cytopathic effect , and more biological activity of IFN-γwas detected in the infected B16F10 cells' supernatant with the increased MOI values. Therefore, the recombinant adenovirus constructed in this study can express and secrete human IFN-γ.

其中的1号重组病毒纯化后，按不同的MOI值感染复制缺陷型腺病毒不能在其中增殖的B16F10细胞，未出现细胞病变效应（cytopathic effect，CPE），而上清中的γ-干扰素活性却随MOI值增大而升高：这证实构建的复制缺陷型腺病毒是能表达并分泌人γ-干扰素的。

MLPA detected deletions and duplications in 2 probands, which were not detected by DHPLC.

与DHPLC法和传统的多重PCR方法相比，MLPA法检测DMD基因的缺失/重复突变位置更为准确。

Results Well-resolved, reproducible 2-DE profiles of human colon carcinoma tissues and paired normal tumor-adjacent colon tissues were obtained. For colon carcinoma tissues, a total of (980±28) spots were detected; for paired normal tumor-adjacent colon tissues,(860±45) spots were detected. For normal tumor- adjacent colon tissues, the average deviation of spot position was (10.487±0.11) mm in IEF direction and (0.944±0.12) mm in SDS-PGE direction; for colon carcinoma tissues the average deviation of spot position was (0.654±0.11) mm in IEF direction and (1.20±0.22) mm in SDS-PGE direction. Number of differentially expressed proteins was 72.00±12.34 between colon carcinoma and paired normal tumor-adjacent colon tissues.

结果 建立了结肠癌及正常肠组织的双向凝胶电泳图谱，其中癌旁正常结肠组织和结肠癌组织电泳图谱中平均蛋白质点数分别为860±45和980±28；癌旁正常结肠组织和结肠癌组在IEF方向上的平均偏差分别为(0.487±0.11)mm和(0.654±11)mm，在SDS-PAGE方向上的平均偏差分别为(0.944±0.12)mm和(1.20±0.22)mm，结肠癌与癌旁正常结肠组织的差异表达蛋白质点数为72.00±12.34。

Zhongding Detected Detected by virtue of professional and technical personnel as well as professional testing laboratory, has won the customers trust agreement.

中鼎检测凭借检测专业的技术人才以及专业的检测实验室，赢得了客户的一致信赖。

It seems likely that Kraft will return with a better offer.

看起来卡夫要重新考虑一个更好的出价了。

But he'd rather burn off my feelers and watch me squirm.

可他却放任那个家伙对我为所欲为！