查询词典 detected

The recombinant viruses, with the gene of reference RV strain VP2, field strain T114 VP6 and T73 G1-serotype VP7, respectively, co-infected Sf9 cell at a multiplicity of infection of 0.5. Harvested the supernatants of culture when the cells were lysed fully (about 7-10 days), or harvested cells before cell lysates (about 4-5 days after infection) and then treated the cells with lysing solution. The supernatants of and cell lysates were ultracentrifuged twice with 40% sucrose cushion at 93 000g, and the sediment products were harvested with TNC buffer. The purified VLPs samples were separated by SDS-PAGE, and then stained by Coomassie blue and silver nitrate and detected by Western blot to analyze the composition of VP2, VP6 and VP7 and their specificity. The morphologic structure of recombinant VLPs was detected by EM.

含标准株VP2、地方株T114 VP6和地方株T73 G1型VP7蛋白编码基因的重组病毒以0.5 MOI共感染Sf9细胞制备2／6／7病毒样颗粒，待细胞完全裂解后收获培养基上清或感染后5天细胞裂解前收获细胞并用裂解液裂解，培养基上清和细胞裂解液样品两次40％蔗糖垫93000g超速离心，沉淀样品经SDS-PAGE胶分离，用考马氏亮兰和硝酸银染色以及Western blot检测样品中VP2、VP6和VP7蛋白的组成以及特异性，电镜检测重组病毒样颗粒的形态结构。

The amount of bacteria in cuhure was detected, the concentration of ammonium oxalate was detected through photometric determination of oxalic acid by its catalytic reaction of methyl red by potassium chromate.

经过72h培养，所有培养基中的草酸浓度都有不同程度的下降，但乳酸菌组的降幅明显大于对照组，其中嗜酸乳杆菌组的降幅最大。

The model of ECV-304 cell oxidative stress injury was established by hydrogen peroxide (H2O2).Then EPZ-contained blood serum was taken as experimental drug. The adherence of monocytes to endothelial cell were measured by method of rose Bengal. The total RNA of cells was extracted. The intercellular adhesion molecule-1 (ICAM-1),vascular cell adhesion molecule-1 (VCAM-1) and MCP-1 mRNA expression in cells were detected by RT-PCR.MCP-1 protein expression were detected by ELISA.

采用过氧化氢建立离体培养的血管内皮细胞(ECV-304)氧化应激损伤模型，取健康大鼠每日灌服不同剂量的鲜姜有效部位(200，400，800 mg·kg-1)或阳性对照药洛伐他汀(40 mg·kg-1)，取含药血清作为受试药物，用孟加拉玫红染色法测定单核细胞与内皮细胞的黏附力；逆转录聚合酶链反应测定细胞内细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子(VCAM-1)以及单核细胞趋化蛋白(MCP-1)的mRNA表达水平；酶联免疫吸附法测定MCP-1蛋白表达水平。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Based on joint several edge detection methods, the edge was detected from SAR image by using Haralick algorithm and general fuzzy operator algorithm, and then the detected edge was subtracted from SAR image, then Lee filter was used to filter the speckle image without edge, at last the edge was added to the filtered image.

常规滤波算法在滤除噪声的同时也带来了图像边缘模糊问题，利用多种检测图像边缘算法的有机组合，首先提取出图像边缘，然后将边缘从图像中剔除，对剩下的无边缘噪声图像用改进Lee滤波法进行噪声抑制，再把边缘图像加入，最后得到了能保持边缘的SAR图像相干斑滤除图像。

Then we detected the quantity of 5-HT and 5-HIAA in colon,serum and hippocampus by high-pressure liquid method, detected the quantity of 5-HT in amygdale and hypothalamus by immunohistochemistry method.

蜘蛛香环烯醚萜各组均可明显降低结肠中5-HT的含量(P.05)，24.92mg/kg组和6.23mg/kg可显著降低5-HT/5-HIAA比值(P.05)，24.92mg/kg可明显降低结肠中5-HIAA的含量(P.05)。

Then we detected the quantity of 5-HT and 5-HIAA in colon,serum and hippocampus by high-pressure liquid method, detected the quantity of 5-HT in amygdale and hypothalamus by immunohistochemistry method.

蜘蛛香环烯醚萜各组均可明显降低结肠中5-HT的含量(P.05)，24.92mg/kg组和6.23mg/kg可显著降低5-HT/5-HIA(来源：Abc6777BC论文网www.abclunwen.com)A比值(P.05)，24.92mg/kg可明显降低结肠中5-HIAA的含量(P.05)。

The proteins in milk and dairy products were mostly eliminated by the precipitators. Three aseptics and two sweeteners were separated on a C18 column with the mobile phase of methanol -0.05mol/L potassium dihydrogen phosphate under gradient elution. With a diode array detector, acesulfame, benzoic acid, and sorbic acid were detected at 230 nm and sodium saccharin and aspartame were detected at 210nm.

通过加入适量沉淀剂除去样品中绝大部分蛋白质后，采用C18色谱柱分离，以甲醇－0.05mol/L磷酸二氢钾溶液为流动相梯度洗脱，用二极管阵列检测器于230nm波长处检测安赛蜜、苯甲酸和山梨酸，于210nm波长处检测糖精钠和阿斯巴甜。

Six and twelve boors after sepsis, leptin levels in liver homogenate were detected by radioimmunoassay serum alanine aminotransferase and 4 enzymes in liver homogenate. myeloperoxidase, glutathin-S-transferase, xanthine oxidase and superoxide dismutase, all related with synthesis of free radicals, detoxication and purine metabolism, were detected by 96 well spectrophotometry. H-E staining was used to examine the histopathologic changes of liver.

于脓毒症后6 h和12 h，采用放射免疫法测量肝组织匀浆液中Leptin水平，采用96孔分光光度法检测血清丙氨酸氨基转移酶及髓过氧化物酶、谷胱甘肽－S转移酶、黄嘌呤氧化酶、超氧化物歧化酶等4种与自由基合成、解毒和嘌呤生成代谢相关的酶的水平，同时以H-E染色法观察肝组织病理学改变。

Tartaricum, moreover, it was much more obvious in tissues of F. tartaricum. EST-1 and EST-2 existed in leaf and caudex of F. cymosum 2. EST-3 and EST-5 were found in all the tested leaves and the bands in F. cymosum 1 were clearest. For CAT, only 3 bands were detected in all tissues, CAT-1 appeared in the leaves of three buckwheat and clearer in F. cymosum and F. cymosum 1. CAT-2 was detected in different location in leaves and caudexes. CAT-3 existed in the leaves though it was unclear.

EST-1及EST-2只出现在金荞麦2号茎和叶中，EST-3和EST-5只存在于叶片中，并且在金荞麦1号中的带最强，EST-4存在于茎和叶片，在金荞麦1号最强；CAT只有3条带，其中CAT-1出现在叶片中，在苦荞麦1号及金荞麦1号表达较强；CAT-2出在现在茎和叶片，并且茎，叶位置不同；CAT-3只存在于叶片中，非常微弱。

It seems likely that Kraft will return with a better offer.

看起来卡夫要重新考虑一个更好的出价了。

But he'd rather burn off my feelers and watch me squirm.

可他却放任那个家伙对我为所欲为！