查询词典 detected

Methods: Eleven B-CLL patients were studied. Leukemic lymphocytes with (n=8) or without (n=3) P2z receptors were exposed in vitro to ATP, benzoylbenzoic-ATP, 2-methylthio-ATP(2MeSATP), adenosine-5′-［γ-thio］ triphosphate, and other nucleosides for 8h. Apoptosis was detected by electron microscopy, agarose gel electrophoresis, and quantitative TdT assay. Results:Apoptosis was detected only in leukemic lymphocytes with P2z receptors. By using the quantitative assay, ATP-inducing DNA strand breaks were found to occur specifically for BzATP, ATP and 2MeSATP, but not for ATP-γS and other nucleosides.

将表达P2z受体［P2z］与不含P2z受体［P2z］的两组CLL细胞分别同1.0mmol/L三磷酸腺苷体外培养8小时，以电镜、DNA凝胶电泳和定量DNA 3′端TdT法检测细胞凋亡；并对ATP、苯甲酰苯甲酸ATP、2-甲基硫ATP(2MeSATP)、γ-硫代ATP及其它核苷的诱导效应和氧化型ATP、1-［N，O-二(5-异喹啉碘酰基)N-甲基-L-酪氨酰］-4-苯哌嗪KN-62）的抑制效应做定量研究。

Methods DNA aneuploid and TA were detected respectively in 165 cases of malignant pleural effusion and 127 cases of benign dropsy of chest (benign group, including tuberculous,other effusion and transudate of chest) by FCM and TRAPHybELISA.Carcinoembryonic antigen was also detected in the two groups.The difference of DNA aneuploid and TA between these two groups was analyzed.

恶性积液165份、良性积液127份(包括结核性、其他渗出液、漏出液)，应用流式细胞术检测DNA异倍体、端粒末端重复序列扩增—杂交—酶联免疫分析技术检测TA，同时检测临床常用的癌胚抗原，比较各类积液中上述3项指标检测结果的差异。

We detected the distribution of lipases in 35 Aeromonas isolates by PCR andphenotypic test using tributyrin as substrate. The positive rates were 100% and 83%,respectively. The lipase activity or associated genes could be detected in the two avirulentstrains, A. hydrophila W1 and A. hydrophila 4332. It suggested that lipase might not be animportant factor in pathogenic mechanism of A.

对本实验室保存的35株气单胞菌分离株进行了脂酶的表型检测和PCR检测，结果分别为83％和100％，特别是嗜水气单胞菌无毒株W1和Ah4332也能检测到脂酶活性或基因，表明脂酶在该菌的致病机制中不是决定性因子。

To elucidate the possible mechanism of differentiation and /or apoptosis in NB4, K562 cells induced by tributyrin, a histone deacetylase inhibitor, the level of acetylated histone H3 was detected by Western blot, p21~ WAF1expression was detected by semi-quantitative RT-PCR.

为了探讨组蛋白去乙酰化酶抑制剂三丁酸甘油酯(tributyrin，TB)诱导NB4、K562白血病细胞分化和凋亡的作用机制，利用Westernblot方法及逆转录聚合酶链反应检测TB作用前后NB4、K562细胞组蛋白H3乙酰化水平以及p21WAF1表达量的改变。

To elucidate the possible mechanism of differentiation and/or apoptosis in NB4, K562 cells induced by tributyrin, a histone deacetylase inhibitor , the level of acetylated histone H3 was detected by Western blot, p21(superscript WAF1) expression was detected by semi-quantitative RT-PCR.

为了探讨组蛋白去乙酰化酶抑制剂三丁酸甘油酯(tributyrin， TB)诱导NB4、K562白血病细胞分化和凋亡的作用机制，利用Western blot方法及逆转录聚合酶链反应检测TB作用前后NB4、K562细胞组蛋白H3乙酰化水平以及p21(上标 WAF1)表达量的改变。

Methods MCF7 cells were treated by Trollius flavonoids of different concentrations for 24 h. The inhibition effect of cell proliferation was detected by CCK8 method, and the telomerase activity was detected by flow cytometry.

不同浓度金莲花黄酮作用于MCF7细胞24 h，以CCK8法检测细胞增殖抑制作用、流式细胞术检测端粒酶活性。

Methods Serum CEA and CA19-9 were detected by radio immunoassay in 38 patients of observation group with cholangiocarcinoma and carcinoma of ampulla before endoscopic retragrade cholangiopancreatography, took 5 ml bile to detecte CEA and CA 19-9 While ERCP, and took endoscopic biopsy for histopathological with the help of wise, recorded the feature of imaging. Bile CEA and CA19-9 were detected in 30 patients with unmalignant of contrast group in the same way.

观察组38例肝外胆管癌及壶腹癌患者在行ERCP检查前检测血清CEA及CA19-9，行ERCP检查时经造影导管抽取胆汁5ml用放免方法检测CEA及CA19-9，同时在导丝引导下自胆管咬取活检行病理检查，并记录影像学特征；同样，对照组30例非恶性病变患者在行ERCP检查时抽取5ml胆汁检测CEA及CA19-9。

Any organic acids couldn't be detected from the untransformed Scout66. After aluminium stress, malate and citrate could be detected from the transformed Scout66 and the highest amounts were 0.162μmolg-1 root fresh wt 24h~(-1) and 0.312μmolg-1 root fresh wt 24h~(-1) respectively.

Scout66的对照材料未检测到任何有机酸盐的信号，在转化植株中则检测到了苹果酸盐和柠檬酸盐分泌的信号，且分泌的最高量分别可达0.162μmolg-1 root fresh wt 24h~(-1)和0.312μmolg-1 root fresh wt 24h~(-1)。

Methods The animal model of skin avulsion injury was replicated, and the expression of CD18mRNA in avulsed skin tissues was detected by RT-PCR method; and the amount of neutrophilic granulocyte in tissues was detected by MPO method in order to analyze the tendency of neutrophil of avulsed tissues after trauma.

复制猪皮肤撕脱伤模型，采用RT-PCR方法检测伤后不同时间撕脱组织中CD18mRNA的表达；用髓过氧化酶（Myel operoxi daBe enzyme，MPO）法测定组织中中性粒细胞数量，研究伤后撕脱组织中中性粒细胞的趋化情况。

Methods: Renal IRI models were made in rats(the right kidney was resected, and the left renal artery was closured by a clip for 1 hour and then was loosened to reperfusion). The concentration of TNF-a, IL-8 and IL-6 were detected by double antibody sandwich enzyme-linked immunosorbent assay. Using biochemical analyzer, concentration of serum urea nitrogen and creatinine were detected.

采用大鼠肾I-R模型(将大鼠右肾切除，夹闭左肾蒂1h再，松开使左肾再灌注)，用双抗体夹心ELISA法动态检测肾组织和血液中TNF-a、IL-8、IL-6含量，以及用生化分析仪测定SUN、Scr等指标变化。

It seems likely that Kraft will return with a better offer.

看起来卡夫要重新考虑一个更好的出价了。

But he'd rather burn off my feelers and watch me squirm.

可他却放任那个家伙对我为所欲为！