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The loss of the diesel soot and smoke Cha Cha PAHs in the leaching rate of the most important factor is the length of brewing time;PAHs were detected in the loop on the small number of PAHs,5 of the ring PAHs were only in the low level of test,and the experiment detected no PAHs, detected no 6 of the ring,regardless of diesel smoke or soot Cha Cha,the concentration of PAHs has the highest concentration of dihydrogen Acenaphthene;On the influence of the number of brewing tea,the total concentration and the leaching rate of PAHs in the soot and smoke Chai tea are lower than on the influence of the brewing time and temperature.

其中影响煤烟茶和柴烟茶中PAHs浸出率的最主要因素都是冲泡时间的长短;检测出的PAHs都集中在环数较少的PAH,5环的PAHs仅在较少的试验水平中检出,无6环的PAHs检出,无论煤烟还是柴烟茶中PAHs的浓度都以二氢苊的最高;在冲泡次数影响下,煤烟茶、柴烟茶中PAHs的总浓度和总浸出率,比在冲泡时间和温度影响下要低。

In the model of tumor cells metastases with cell line A673, 10〓 tumor cells can be detected after only one ampliation with outer primers, which means we can find 1 tumor cells in 10〓 normal PBMC, and enlarged again with inner primers, 10〓 tumor cells can be detected, which means we can find 1 tumor cell in 10〓 normal PBMC. In the simulative model of tumor cells metastases with RNA, 10〓 tumor cell can be detected that is to say we can find 1 tumor cell in about 10〓 normal PBMC. In all 15 samples of 11 patients, 2 patients'EWS/FLI1 expressions were negative, no evidence of metastases was found followed up by 8 months and 30 months respectively. Semi-quantitative study was used to detect the expression level of EWS/FLI1 mRNA in the other 9 positive patients, and metastases occurred in 6 high-level expression patients in 2-24 months after the operation, no evidence of metastases in the other 3 low-level expression patients up to now.

应用A673细胞系建立的肿瘤转移模型,单独应用外引物进行一次扩增,可以检测到10〓级肿瘤细胞,即可以从10〓个正常细胞中检测到1个肿瘤细胞;而在此基础上再次应用内引物扩增,则可以检测到10〓级肿瘤细胞,即可以从10〓个正常细胞中检测到1个肿瘤细胞;在用RNA模拟的肿瘤细胞转移模型中,两次扩增可以检测到10〓级肿瘤细胞,即可以从10〓个正常细胞中检测到1个肿瘤细胞。11例患者的15份标本中,2例没有检测到EWS/FLI1 mRNA的表达,分别随访8个月和30个月,没有发现临床转移的证据;对其余9例阳性表达的病例进行半定量的研究,6例EWS/FLI1 mRNA高表达的病例,分别在术后2-24个月发生转移,而3例EWS/FLI1 mRNA低表达的病例,则至今没有发现转移的证据。

Methods 60 patients with newly diagnosed ITP, their serum TPO concentration were detected by double-antibody sandwich enzyme-linked immunosorbent assay; and the bone Marrow megakaryocytes were recorded; HP was detected by 14C urea breath test. The control group of 40 cases was also detected by above methods.

60例初治ITP患者,在接收治疗前采用双抗体夹心酶联免疫吸附法检测血清TPO水平,计数骨髓涂片的平均巨核细胞数量,用14C尿素呼气试验检测患者HP感染情况。

In test group fibroblast growth factor immunoreactivity was detected in 89% of the samples, mRNA was detected in 20% of the samples. In control group no fibroblast growth factor immunoreactivity and mRNA was detected.

结果:实验组30例免疫组化和原位杂交的阳性率分别为89%和20%,对照组6例免疫组化和原位杂交均为阴性,两组免疫组化方法检测阳性率有显著差异。

Results toxic test and ld 50 test showed that nata de coco strain was free from toxin.quality control system has not been established and sanitary facilities were incomplete in most fac-tories.totally54samples of nata de coco were hygienically determined.the pollution level of elements of pb,as,nitrate and nitrite was low,but chromium was relatively higher.no aflatoxin b 1 and pathogenic bacteria were detected.the residual hydrogen peroxide were detected from15out of the18samples detected.

结果 菌种毒力试验和急性毒性试验(ld 50 )显示为无毒物;多数企业未建立卫生管理制度,卫生设施不完善;采集54份椰果样品做卫生学检测,铅、砷等重金属元素、硝酸盐、亚硝酸盐污染水平较低,铬含量相对较高,未发现黄曲霉毒素b 1 和致病菌的污染;18份椰果样品中有15份残留过氧化氢。

For different FAB subtypes,translocation was most common in M3 patients,detected in 58%;deletion was most common in M1,detected in 18%;and trisomy was most common in M5,detected in 29%.

在不同的FAB亚型中,M3患者发生染色体易位的比例最高,为58%;M1患者中发生染色体丢失的比例最高,为18%;而M5患者中发生三倍体的比例最高,为29%。

Isolations of the s.aureus insoluble peptidoglycan:(1) the s.aureus insoluble peptidoglycan was gained by cold, hot and hypersound affection repetat of s.aureus(ATCC25923);(2) the s.aureus insoluble peptidoglycan was gained by 10% trichloracetic acid, 5% trichloracetic acid,NH4HCO3 buffer with trypsin digestion of s.aureus(ATCC25923) in order. 2. Isolations of the s.aureus soluble peptidoglycan and quality control:(1) the s.aureus soluble peptidoglycan was released by short dose benzylpenicillin sodium and purified by sephadex G-100.(2) The quality control in the process of isolation: the soluble peptidoglycan was detected by SLP reagent kit; the LPS was detected by kinetic turbidimetric limulus test;and the protein was detected by improved LOWRY reagent kit.

金黄色葡萄球菌可溶性肽聚糖的分离提取及其质量控制(1)采用青霉素钠诱导法和葡聚糖G-100(Sephadex G-100)凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖;(2)金黄色葡萄球菌可溶性肽聚糖提取过程中的质量控制:采用SLP试剂盒对提取物定性检测,应用动态浊度法鲎试验测定提取物中内毒素含量,采用改良LOWRY试剂检测提取物中蛋白的含量;(3)采用青霉素诱导和Sephadex G-100凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖的条件:青霉素使用量、加入青霉素后孵育时间和菌液浓度等优化选择。

Results Multidrug resistance could be detected in the tumor tissue after the injection of retrovirus around and into it.After filtration and centrifugalization,the concentrated supernatant was 2 times efficient than the original supernatant and P-GP expression was detected in 60% of the tumor cells.mdr1 mRNA expression in the tumor tissue was significantly increased after gene transfer as detected by RT-PCR.

结果 携带mdr1基因的逆转录病毒上清液注射后肿瘤模型产生多药耐药性,经过滤和离心浓缩的病毒上清液60%细胞表达P-GP,是病毒原液基因转移率的2倍;RT-PCR表明基因转移后,肿瘤组织内mdr1 mRNA的表达量明显增加。

The expression of NSE was detected in cortex of adrenal cortical hyperplasia, cortical adenomas and pheochromoeyte carcinoma, hut not in that of pheochromocytoma and normal adrenal. The expression of SYN was detected in cortex of corticohyperplassia, cortical adenomas, medull neoplasm, chromophile tumor and pheochromocyte carcinoma, but not in that of cortical adenocarcinoma and normal adrenal. The expressions of CgA, NSE and SIN were detected in the medulla of all cases. Conclusion The characteristic of CgA, SYN and NSE in cortex and medulla of adrenal are useful in the differential diagnosis of tumor and hyperplasia in the adrenal.

结果 肾上腺皮质腺瘤、皮质腺癌、嗜铬细胞瘤和嗜铬细胞癌的皮质中均可见CgA染色阳性细胞,皮质增生、髓质增生和正常肾上腺病例皮质CgA染色阴性;肾上腺皮质增生、皮质腺瘤、皮质腺癌、髓质增生和嗜铬细胞癌NSE染色呈阳性,嗜铬细胞瘤和正常肾上腺皮质NSE染色呈阴性;皮质增生、皮质腺瘤、髓质增生、嗜铬细胞瘤和嗜铬细胞癌的皮质中SYN染色呈阳性,皮质腺癌和正常肾上腺皮质SYN染色呈阴性;所有病例髓质CgA、NSE和SYN染色均呈强阳性。

Results Localization diagnosis:2 of 3 cases were detected by CEUS(66.7%); All of 3 cases were detected by pancreatic thin-section dynamic real-time enhanced spiral CT(100%); All of 2 cases were detected by ASVS,but 1 case was located inexactly(50%); All of 4 cases were detected by IOUS with operation exploration(100%).

结果 定位诊断:3例行超声造影,检出2例(66.7%);3例行胰腺薄层螺旋CT动态实时增强扫描,均获检出(100%);2例选择性动脉钙刺激静脉采血测定胰岛素,均获检出,但其中1例定位不准确(50%);4例行术中超声+手术探查,均获检出(100%)。

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