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AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞；以不同浓度的LPS(0μg/ml，0.01μg/ml，0.1μg/ml，1μg/ml，10μg/ml)和不同作用时间(0min，5min，15min，30min，60min，120min)分别刺激小室培养的细胞单层，观察NF-κB的核内浓度及NO合成量的动态变化，选择LPS的最佳用量和作用时间；然后分成四组实验，设正常对照组，LPS处理组，特异性PKC抑制剂阻断组，NF-κB抑制剂阻断组；收集培养的单层细胞及培养液；采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平；免疫组化法检测NF-κB的核内移位变化；硝酸还原酶法测定细胞培养液中NO含量；吖啶橙染色观察凋亡细胞的形态学变化，凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

The somatic hybrids usually showed specific bands and combined all the bands of both parents. In combinations, some new bands were detected, and occasionally specific bands of parents were lost, which indicated that chromosome recombination and exchange happened after fusion. Additionally, in some combinations, specific bands from leaf parent were detected, which revealed the plants were of putative hybrid origin, because leaf protoplast cannot redifferentiate by both single culture and co-culture.

体细胞杂种一般表现为具有双亲的特征带，且综合双亲的所有带，但在部分杂种中检测到了双亲都没有的新带，也发现有丢失亲本的特征带或共有带的现象，说明融合后染色体发生了重组和交换；有的引物只检测到叶肉亲本的特征带，根据柑橘叶肉细胞无论单独培养还是共培养均不能再生的事实，可推测其为体细胞杂种或二倍体叶肉亲本型胞质杂种。

TUNEL method was used to detected the apoptosis in the artery. Results:①The MSC cocultured with VSMC expressed smooth muscle a-actin, calponin and CD31, no cells positive for calponin and CD31 were detected in the control group; and a lot of filaments were observed in the co-cultured MSC by electron microscopy.②We gain dual-stable expression of AT2R gene medicated by doxycycline regulatable system of mesenchymal stem cells.

单独培养及VSMC条件培养液培养的MSC仅SM-α-actin表达阳性；(2)Dox-on系统由四个质粒组成：调节质粒pUHD17-1 hyg，荧光报告质粒pUHC 13-3，筛选质粒pSV2neo以及含目的基因AT2R的应答质粒pUHD10-3/AT2R，以上质粒经鉴定均与其背景资料相符合，可用于实现目的基因片段在靶细胞中可调控表达研究；(3)本实验通过连续两个回合的转染及抗生素压力筛选，经Dox诱导表达后获取出低背景、高诱导表达AT2R基因的克隆。

To resolve the discrepancy between what was detected in chicks and not detected in rodents, the Reh laboratory conducted a systematic analysis of the response to injury in the mouse retina, and the effects of specific growth factor stimulation on the proliferation of Müller glia cells.

要解决之间的差异是什么检测雏鸡，并没有发现在啮齿动物，在Reh的实验室进行了系统的分析的反应损伤的小鼠视网膜的影响特异性生长因子刺激对米勒扩散的胶质细胞。

The level of ifn γ secreted from splenocytes of immunized mice after specific antigen stimulation was detected by a cytopathic effect reduction assay and the splenocytic proliferation of immunized mice in response to antigen restimulation was detected by mtt assay.

分离免疫小鼠的脾淋巴细胞，体外用抗原再刺激，mtt方法检测ifn γ产生水平和mtt法检测淋巴细胞增殖，并对脾脏细菌负荷计数。

Methods Seral anti-P 53 antibody was detected with immuno-PCR.The expression of P 53 in tissue was detected with enzyme imunohisto-chemistry technique.

采用免疫PCR方法检测血清抗P 53 蛋白抗体，酶免疫组化方法检测组织P 53 蛋白表达。

Objective To present a new serological method for diagnosis of breast cancer. Methods Seral anti-P 53 antibody was detected with immuno-PCR.The expression of P 53 in tissue was detected with enzyme imunohisto-chemistry technique.

目的 探讨乳腺癌患者P 53 蛋白及抗体表达情况方法采用免疫PCR方法检测血清抗P 53 蛋白抗体，酶免疫组化方法检测组织P 53 蛋白表达。

Model of animals were established by putting rats in a container with slippy inner wall and half-full of water in it, letting them swimming freely till died of being drowned. Each rat from different groups was killed by breaking its neck except drowning group, then BW and SO_2 was detected, and its chest was cut open rapidly, lung was taken out and weight was measured accurately to count lung coefficient. Its lung appearance was observed and lung tissue was fixed with formalin, which was made into pathology slices with HE staining, and pathology change of the lung tissue was observed;Expression of IL-8 and IL-10 in lung tissue was detected with Immunocytochemistry, and correlation between them was analyzed .

动物模型制作采用将大鼠放入盛有黄河水的内壁光滑的容器内，让其自由泳动自然溺死，除溺水致死组外，各组动物分别在规定的时间点断头处死，行体重和血氧饱和度等指标检测，并在冰盘中迅速开胸取肺，准确称重以计算肺系数，观察各组大鼠肺脏外观，取肺组织福尔马林固定，制成病理切片HE染色，光镜下观察肺组织病理改变；用免疫组化的方法检测肺内IL-8、IL-10的表达，并对其相关性进行分析研究。

Materials. For example , the specifical chemical ingredient in air or liquid are detected by this kind of sensors ,and are converted into detected current or voltage signal.

化学传感器是一类专门用于检测、感知化学物质的特殊传感器，通常用于检测气体或液体中的特定化学成分，并将该化学成分的浓度信号转换为可检知的电流或电压信号。

The activity of erythrocyte AR and 〓-ATPase, the level of the plasma NO and NOS, the content of sorbitol in sciatic and erythrocyte were detected by spectrophotography; The level of ET in plasma were detected by radioimmunassay .

荧光法测定红细胞AR活性；分光光度法测定红细胞膜〓-ATPase活性、血浆NO及NOS水平、坐骨神经及红细胞内山梨醇含量：放射免疫方法测定血浆ET水平：RT-PCR方法半定量坐骨神经内ARmRNA水平。

It seems likely that Kraft will return with a better offer.

看起来卡夫要重新考虑一个更好的出价了。

But he'd rather burn off my feelers and watch me squirm.

可他却放任那个家伙对我为所欲为！