查询词典 detected

The general situations of the eye were observed and the corneal thickness were measured with ultrasonic corneal pachymeter after the animal models was established. After a week, the corneas were removed after the experimental animals are put to death. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution , HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

术后观察眼球大体情况、测量角膜厚度。1周后处死实验动物取角膜，用茜素红-台盼蓝染色染色行角膜内皮细胞计数；用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；实时荧光定量PCR检测AQP-1mRNA在角膜内皮细胞表达的改变；并于正常对照组角膜比较。

Results Sera from 150 patients with schistosomiasis were detected, the average sensitivity was 84.7%(72.0%～91.0%). No false positive reaction was detected in 40 normal controls and none but 2 patients(2/10) with paragonimiasis showed cross reactions among 74 cases with other parasitic infections.

结果 用于检测 15 0例血吸虫病患者的循环抗原，其敏感性平均为 84.7%(72 。0 %～ 91.0 %)，40例正常人未出现假阳性反应，74例其他寄生虫感染者中，有 2例并殖吸虫病患者阳性，其余均未出现交叉反应。

Methods: The motion of parasternal papillary muscle anterior wall, inferior wall, posterior wall, and lateral wall were detected by 2DE at short axis view of left ventricle before PTCA, 3 d and 1-3 months after PTCA in 33 cases of patients with coronary heart disease, who received PTCA+stent at selected time. Peak systolic velocity of subendocardial, subepicardial and middle layer myocardium, transmural velocity difference of the same segments and Outer lateral mitral valve annulus were also detected at apical 4 chamber view by pulsed wave tissue Doppler imaging.

对33例择期行PTCA+支架术的冠心病患者用HP5500型超声诊断仪，于PTCA术前及术后3d、1-3个月对胸骨旁左心室乳头肌短轴切面的前间壁、下壁、后壁、侧壁行二维超声心动图(2DE)检测各节段的室壁运动情况，用脉冲组织多普勒成像检测上述各节段的内、外膜下及中层心肌的收缩期峰值速度，内外膜下心肌的收缩速度差及心尖四腔观二尖瓣外侧环的收缩期峰值速度。

Bacteriological examination, Pasteurellosis dye concentration polarization can be detected in the bacteria, but this disease can be detected in a similar kind of Brevibacterium sp.

细菌学检查，巴氏杆菌病可检出两极浓染的杆菌，而本病可检出类似球菌样的短杆菌。

MethodsThe transplanted tumor model of the mouse S180 sarcoma was established.Fifty mice were randomly divided into five groups,the control group,the CTX group,the Gecko group.They were treated respectively with oral administration of saline,and intraperitoneal injection of CTX 100 mg/kg only one time,oral administration of Gecko in doses of 13.5,9,4.5 g/kg,one time a day.After 14 days,the anti-tumor activity was evaluated by tumor tissue weighing. The impact on immune organ was detected by accounting thymus index, spleen index and the number of peritoneal macrophage which phagocytose chicken red blood cells.The protein express of vascular endothelin growth factor and basic fibroblast growth factor were detected by SABC immunohistochemistry.

方法建立移植瘤小鼠S180肉瘤模型，将50只雌鼠随机分为对照组、环磷酰胺组、壁虎高组、中组、低组共5组，分别给于生理盐水灌胃1次/d，CTX（100 mg/kg）腹腔注射1次，壁虎高、中、低组（13.5，9，4.5 g/kg），每天灌胃1次。14 d后，称取荷瘤小鼠瘤重、胸腺重、脾脏重，计算抑瘤率、胸腺指数、脾脏指数，观察腹腔巨噬细胞吞噬鸡血红细胞实验指标；用SABC免疫组化法检测血管内皮生长因子、碱性成纤维细胞生长因子的蛋白表达，TUNEL方法检测细胞凋亡率。

1D-SDS-PAGE eletrophoresis and molecular markers were employed to survey the Waxy proteins and Waxy genes in 312 winter wheat cultivars and lines from Chinese winter wheat and U.S.A. and Austrilia, 60 lines selected from the cross of Baihuomai and Norin 67 which was detected preliminarily to be waxy wheat with Iodine-dyeing-seed-section , 120 F2 plants from cross of Lu935031(Jimai19) and Yumai 47 . Amylose content and RVA properties were also recorded. The major results were presented below:1 The components of waxy protein subunits could be detected precisely by 1D-SDS-PAG Eelectro- phoresis.

以国内外冬播小麦品种312份、从白火麦×Norin 67中选育出经碘染籽粒剖面初步鉴定为糯性小麦的品系60份以及鲁935031×豫麦47的F2代120个单株为材料，利用1D-SDS-PAGE电泳技术研究了Waxy蛋白亚基组成，并利用分子标记检测了相应Waxy基因；检测了部分材料的直链淀粉含量和RVA特性等，分析了国内主要麦区小麦淀粉特性；结合生化标记、分子标记和淀粉特性三者各自的优势，探讨了快速、准确、简便筛选具有优良淀粉品质的小麦资源和品种的途径。

Catenin was detected in suprabasal, epidermal basal layer, placode, hair bud in early phase(E68～93),and expressed strongly in epidermal basal layer, placode, and hair bud, in suprabasal expressed less strongly; in metaphase(E94～184),β-catenin was detected in epidermis, hair peg, and in suprabasal, epidermal basal layer, hair follicle bulge, inner root sheath, outer root sheath, follicular infundibulum expressed less strongly; in late phase(E184～225),β-catenin expressed weakly in epidermal basal layers, while expressed strongly in epidermal keratinocytes.

结果显示：β-catenin在胎牛蹄边缘毛囊形态发生早期(68～93天)表达于表皮、基底层、基板和毛芽中，其中基底层、基板、毛芽中呈强阳性，表皮中呈中阳性；在毛囊形态发生中期(94～184天)表达于基底层、表皮和毛钉中，其中基底层、表皮、隆突区、内外根鞘和漏斗部呈中阳性；在毛囊形态发生晚期(184～225天)只在表皮及基底层有表达，其中基底层呈弱阳性，表皮呈强阳性。

By using β-NADPH-diaphorase histochemical staining technique,wefound for the first time that NOS activity was detected in the epithelium of airpathway and endothelium of vessels but was not detected in the small vessels(ID＜100μm),and the NOS express in pulmonary vascular endothelium of preascitic andascitic broilers were elevated,especially in the endothelium and smooth muscle layer ofsmall vesseles with thicker walls and of muscularized nomuscular artery in asciticbroilers.

本研究从田间自然发生腹水症的肉鸡群中选取正常肉鸡、亚临床腹水鸡和腹水鸡为实验对象，通过比较这三种肉鸡血流动力学、心电图、动静脉血血气、肺外动脉舒张反应性、肺血管结构以及肺组织一氧化氮合酶的活性和分布等方面的不同，探讨了肺动脉舒张能力在肉鸡肺动脉高压形成中的作用和机制。

Even carbon number alkenes, phthalic esters and nitrogen heteroatom-containing components were detected in the elution fractions, while such components were not detected without silica gel column separation, indicating that preseparation is very important for increasing the sensitivity of GC/MS analysis.

各洗脱馏分用GC/MS分析，其可测成分与直接检测相比，多检测出偶碳烯烃、邻苯二甲酸酯类和含氮化合物等，表明合适的预分离对提高GC/MS分析的灵敏度非常重要。

Methods: HPV 16/18 infection was detected by nested-polymerase chain reaction, the p53 mutation was detected by direct sequencing, and the p53 and the HPV 16/18 E6 proteins were studied using immunohistochemistry on 129 pterygial specimens and 20 normal conjunctivas.

本研究利用巢叠式聚合脢锁反应分析自129例罹患翳状赘肉之翳状赘肉组织及20例正常无翳状赘肉的结膜组织检体中，是否有人类乳突病毒16及18型的感染，并利用直接定序的方式检测p53基因是否发生突变，此外亦以免疫组织化学染色法侦测p53蛋白与人类乳突病毒E6蛋白。

It seems likely that Kraft will return with a better offer.

看起来卡夫要重新考虑一个更好的出价了。

But he'd rather burn off my feelers and watch me squirm.

可他却放任那个家伙对我为所欲为！