查询词典 detected

The result showed that: 148 poly morphic hands were detected by use of, 22 RAPD primer and 158 allele were detected by use of 34 SSR primer.

利用筛选出的22条RAPD引物，检测到了148条有多态性的带；利用筛选出的34对SSR引物，检测到158个等位基因。

C2C12 cells were induced to differentiation into muscle cells in vitro and the myogenesis model was detected by inverted microscope, RT-PCR, western-blot, cell immunofluorescence histochemistry, flow cytometry and confirmed to be successful. On the foundation of differentiation model, miR-206 was detected by northern hybridization and the result showed that the expression level increasing gradually during muscle differentiation with a character of phase specificity.

利用C2C12细胞在体外成功诱导其向成肌细胞分化，通过形态学观察，RT-PCR、western-blot、细胞免疫荧光组织化学及流式细胞仪等方法进行检测，证实我们所建立的细胞分化模型是成功的，并观察到随着诱导时间的延长，miR-206在成肌分化过程中表达量逐渐增高的，表达改变具有阶段特异性。

Methods Fifty surgical specimens of uterine leiomyoma and corresponding normal myometrium were collected. The expression of TGF-β1 mRNA was detected by in situ hybridization; while the expression of TGF-β1 and IGF-1R proteins was detected by immunohistochemical S-P method.

采用原位杂交方法检测50例子宫肌瘤及瘤旁正常的子宫肌层中的TGFβ1 mRNA的表达，同时采用免疫组化技术检测相应标本中TGFβ1和IGF-1R蛋白的表达。

Methods The nephelometric analysis was detected to all kinds of viral hepatitis'surm which were determined marker in humoral immunity: ImmunoglobuinIg.Complement(C3.C4).And the marker stand for the hepatocellar damage which contain: Preabumin.Transferrin.Ceruloplasmin.were detected.A statistic alanalysis was made on the results of 8 items specific protein in viral hepatitis' surm together with the results of healthy adults.

采用免疫散射比浊法对各型病毒性肝炎患者血清进行体液免疫指标：免疫球蛋白Ig，补体(C3、C4)和肝损伤指标前清蛋白、转铁蛋白，铜兰蛋白检测，并与正常成人结果为对照进行统计学分析。

METHODS:Nestin expression was detected in 33 cases of neuroepithelial tumors using the immunocytochemical staining. The Nestin positive and negative cells were isolated by magnetic activated cell sorting. Nestin positive cells were culutered, identified, and induced to differentiate. The expression of Nestin protein was detected in Nestin+ cells by RTPCR.

采用免疫组化法检测神经上皮肿瘤组织中Nestin的表达情况，免疫磁珠法分选肿瘤组织中的Nestin+细胞，悬浮细胞培养法进行Nestin+细胞的增殖、分化与鉴定，RTPCR法检测Nestin蛋白在其中的活性表达情况。

With the same DH population and genetic map, a total of 24 QTLs were identified for five traits. Under salt stress, nine (37. 5%) QTLs were detected, while in the nonstress environment, 17 QTLs (70.8%) were detected.

在水稻生长的全生育期对盐胁迫环境与非胁迫环境下5个重要的农艺性状分别进行调查，总共检测到24个QTL，分布在除第9、11号染色体外的各染色体上。

Results Negative expression of Skp2 was detected in all osteochondroma. In contrast, it was detected in 43.75%(21/48)of osteosarcoma.

用免疫组织化学SP法检测16例骨软骨瘤和48例骨肉瘤组织中Skp2和p27蛋白的表达情况。

The levels of serum gonadal hormone were detected by radioimmunoassay. The effects of ZYJN on ovaries and ovarial follicular development were observed by light microscope. The expressed level of LIF was detected by using the immunohistochemical staining technique.

随机分为对照组、PCO模型组、PCO模型组＋助孕胶囊组、PCO模型组＋克罗米酚组，采用放免法测定血性激素水平的变化，光镜观察卵巢、卵泡发育的情况，免疫组化法检测卵巢组织LIF的表达水平。

Methods The PCO rat models, prepared by pregnancy hormone combine with human chorionic gonadotrophin, randomly divided into four group as control, model, Chinese crude drug and western medicine group; The levels of serum gonadal hormone were detected by radioimmunoassay; The effects of ZYC on ovaries and ovarial follicular development were observed by light microscope; And the expression level of TNF-α was detected with the immunohistochemical staining technique.

采用孕激素联合人绒毛膜促性腺激素造模法制备PCO模型大鼠。随机分为对照组、PCO模型组、PCO模型组+助孕胶囊组、PCO模型组+克罗米酚组，运用放免法测定血清性激素水平的变化；光镜观察卵巢、卵泡发育的影响，免疫组化学法检测卵巢组织肿瘤坏死因子α表达水平。

The experimental group corneas were preserved by organ culture for 4 weeks, the corneal thickness was measured with ultrasonic corneal pachymeter. Then every corneas were divided into half -chip, there are 48 half-chip total. It was divided into 4 groups, there are 12 half-chip in every groups. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution, HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

实验组经器官培养保存4周后以角膜测厚仪测量角膜厚度，然后每个角膜被分成两半，共48个半片角膜，再分成4组，每组12个半片。12个半片用茜素红-台盼蓝染色染色行角膜内皮细胞计数；12个半片角膜用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；12个半片角膜用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；12个半片角膜用实时荧光定量PCR检测AQP-1mRNA表达改变。

It seems likely that Kraft will return with a better offer.

看起来卡夫要重新考虑一个更好的出价了。

But he'd rather burn off my feelers and watch me squirm.

可他却放任那个家伙对我为所欲为！