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Methods The 26th exon, the 30th exon and the 21st intron of gene GP Ⅱb in 110 individuals were amplified by polymorphism and sequenced to investigate whether there was linkage among the polymorphisms of the gene. Human platelet antigen-3 (HPA-3) gene frequency was detected by Fok 1 enzyme in 147 patients with hematologic diseases, and was compared with that in 110 normal individuals. Forty-four patients who received apheresis platelet transfusion repeatedly were randomly divided into the HPA-3 homotype group and the control group. The antibodies of the platelet were detected after 3 times of platelet transfusion.

聚合酶链反应-单链构型多态性分析检测110例正常人GP Ⅱb基因第26和30外显子(Exon 26, Exon 30)及21内含子(Intron21)基因多态性,进行基因序分析,研究这些基因多态性是否存在连锁关系;应用Fok1酶切法对147例血液病人人类血小板抗原-3(human platelet antigen-3, HPA-3)基因进行分型,并与110例正常人进行比较;将接受单采血小板输注的44例血液病人随机分为HPA-3同型输注组和对照组,血小板输注3次以后检则血小板抗体。

Angiography detected 42 hypervascular tumors in all 23 patients. CO2US detected 46 and CO2CT 37 liver tumors. No complication was noted in all patients.

在所有23个病人中,肝脏血管摄影司以侦测到42个高血管性的肝肿瘤,二氧化碳显影超音波可以侦测到46个肝肿瘤,而二氧化碳显影电脑断层可以侦测到37个肝肿瘤。

The cell suppression ratio and surviving ratio were detected respectively under conditions of simple hypoxia, or 5mmol/L glucose complicated with hypoxia. The mRNA levels of key enzymes that are involved in regulating glyco-metabolism were detected, including the mRNA levels of pyruvate-kinase, hexokinase, succinic dehydrogenase and isocitric dehydrogenase, and the activity of lactate dehydrognase.

以SMMC-7721人肝癌细胞和L02正常肝细胞作为研究对象,分别在单纯缺氧及加葡萄糖缺氧条件下,观察细胞生长,并检测糖代谢关键酶:丙酮酸激酶(pyruvate-kinase, PK)、己糖激酶(hexokinase, HK)、琥珀酸脱氢酶(succinic dehydrogenase, SDH)、异柠檬酸脱氢酶(isocitric dehydrogenase, IDH) mRNA表达水平和乳酸脱氢酶(lactate dehydrogenase, LDH)活性。

The results were expressed in mean±1SD. Pearson X~2 test and One-way ANOVA test were used. The data analyzed using the SPSS (version 11.5). Results: The sensitivity, specificity, positive and negative value of US for the LPEH model on the children cadaver were 88%, 84%, 79%, 91%, respectively. With regard to the thickness of femora head cartilage, the thickness of the anterior layer or posterior layer, there were no significant differences among three groups. However, the anterior layer was thicker than the posterior layer in three groups. The fluid in hip joint was detected in all of 21 symptomatic hips, which was clear commonly (90%) in early procedure. The amount of fluid in anterior recess showed a positive correlation with age (p .05). No fluid was detected in the asymptomatic and normal hips (2mm). The mean maximum width of inferomedial recess was significantly larger than that of anterior recess (12.50±4.04mm vs.4.35±0.8mm, p 0.05) in the symptomatic hip joints. The echogenic entrapped labral plicaes were demonstrated in the inferomedial recess in all of 21 children with LPEH, whose length and width ranged from 5.3mm-25.0mm (mean,15.6±5.6mm) and from 4.0mm-17.0mm (mean,8.9±7.8mm).

结果1,尸体LPEH髋关节模型的超声诊断敏感性、特异性分别为88%、84%,阳性预测值、阴性预测值分别为79%、91%。2,21例患儿的LPEH患髋(21侧)、健髋(21侧),以及21例正常儿童健髋(42侧)的超声检查显示:髋关节周围软组织及股骨头无形态结构差别;股骨头软骨厚度无统计学差异(3.5±0.5mm vs.3.6±0.4mm vs.3.6±0.5mm,p>0.05);关节囊前层及后层厚度无统计学差异(前层厚度2.79±0.74 mm vs.2.56±0.40mm vs.2.56±0.72mm;后层厚度2.70±0.82mm vs.2.48±0.54mm vs.2.44±0.58mm,p>0.05),但LPEH患髋关节囊前、后层均较后二组有增厚趋势。3,LPEH患髋均存在关节腔内积液,且早期较为清晰;积液以髋关节内下间隙明显,内下间隙较前间隙明显增宽(12.50±4.04mm vs.4.35±0.8mm,p<0.05),其内见嵌顿滑膜唇皱襞呈稍强回声的占位性团块,长约15.6±5.6mm,宽约8.9±7.8mm,90.5%(19/21)嵌顿皱襞内未见血流信号。4,所有LPEH患髋治疗后超声复诊均显示正常。

In contrast to the RplJ, Opa, H.8 antigen, and Laz, which were detected in all seven strains, Opc was only detected in one strain confirming the specificities of the antibodies towards the denatured proteins.

结果在7株菌株都可侦测到RplJ、 Opa、H.8以及Laz,但只有一株菌表现Opc,证明抗血清对变性后的NM表现目标蛋白质具有特异性。

Objective To analyze the CT and MRI manifestations of oral carcinoma,and to evaluate the value of imaging technologies in the diagnosis of oral carcinoma,thus to explore the most reasonable imaging method Methods 46 cases with oral carcinoma,proved by surgery and pathology (2 lip tumors,8 carcinomas of the gingival ridge,5 carcinomas of the buccal mucosa,24 tongue carcinomas,5 carcinomas of the floor of the mouth,2 tumors of the hard palate),were analysed retrospectively32 cases were examined with CT and 14 cases with MRIImaging presentations were compared with surgery and pathology Results Of the 46 cases with oral carcinoma,22 were ulcerative lesion of infiltrative growth,24 were masslike lesion of extruded trowthOf the 32 cases examined with CT scan,27 were detected,5 were missed,the 4 missed cases were confirmed to be superficial ulcers by surgery and pathologyOf the 10 cases with bone destruction examined with CT scan,8 were manifested,2 were missedAnd of the 14 cases with MRI scan,all 14 were detected Conclusion CT and MRI scan are valuable for diagnosis of oral carcinomasThey can manifest the morphology and size of primary tumor,contiguous structures,bone invasion and cervical lymph node metastasisMRI is the optimal imaging method for superficial ulcerative lesion of infiltratibe growth

徐 锋,罗德红,唐威,欧阳汉,周纯武目的分析口腔癌的CT及MRI表现,评价影像检查在口腔癌诊断中的应用价值,探索合理的影像学检查方法。方法回顾性分析经手术及病理证实的口腔癌46例(唇癌2例,牙龈癌8例,颊黏膜癌5例,舌癌24例,口底癌5例,硬腭癌2例),其中32例行CT扫描,14例行MRI扫描,将影像表现与手术所见及病理结果进行对照分析。结果 46例口腔癌中,呈浸润性生长的溃疡型病变22例,外生性生长的肿物型病变24例。CT扫描32例,明确显示病变27例,遗漏病变5例,其中4例为表浅溃疡型病变。CT扫描病例中,10例有骨质侵犯,CT扫描准确评价8例,遗漏2例。MRI扫描14例,均准确评价肿瘤及其与周围结构的关系。结论 CT、MRI对口腔癌原发肿瘤的形态、大小、周围结构侵犯、颈部淋巴结转移的评价有重要价值。对于较小及呈浸润性生长的表浅溃疡型肿瘤,CT扫描尤其是CT平扫难以准确显示,应首选MRI。

Objective To analyze the CT and MRI manifestations of oral carcinoma,and to evaluate the value of imaging technologies in the diagnosis of oral carcinoma,thus to explore the most reasonable imaging method Methods 46 cases with oral carcinoma,proved by surgery and pathology (2 lip tumors,8 carcinomas of the gingival ridge,5 carcinomas of the buccal mucosa,24 tongue carcinomas,5 carcinomas of the floor of the mouth,2 tumors of the hard palate),were analysed retrospectively32 cases were examined with CT and 14 cases with MRIImaging presentations were compared with surgery and pathology Results Of the 46 cases with oral carcinoma,22 were ulcerative lesion of infiltrative growth,24 were masslike lesion of extruded trowthOf the 32 cases examined with CT scan,27 were detected,5 were missed,the 4 missed cases were confirmed to be superficial ulcers by surgery and pathologyOf the 10 cases with bone destruction examined with CT scan,8 were manifested,2 were missedAnd of the 14 cases with MRI scan,all 14 were detected Conclusion CT and MRI scan are valuable for diagnosis of oral carcinomasThey can manifest the morphology and size of primary tumor,contiguous structures,bone invasion and cervical lymph node metastasisMRI is the optimal imaging method for superficial ulcerative lesion of infiltratibe growth

中华硕博网核心提示:徐锋,罗德红,唐威,欧阳汉,周纯武关键词:口腔肿瘤目的分析口腔癌的CT及MRI表现,评价影像检查在口腔癌诊断中的应用价值,探徐锋,罗德红,唐威,欧阳汉,周纯武目的分析口腔癌的CT及MRI表现,评价影像检查在口腔癌诊断中的应用价值,探索合理的影像学检查方法。方法回顾性分析经手术及病理证实的口腔癌46例(唇癌2例,牙龈癌8例,颊黏膜癌5例,舌癌24例,口底癌5例,硬腭癌2例),其中32例行CT扫描,14例行MRI扫描,将影像表现与手术所见及病理结果进行对照分析。结果46例口腔癌中,呈浸润性生长的溃疡型病变22例,外生性生长的肿物型病变24例。CT扫描32例,明确显示病变27例,遗漏病变5例,其中4例为表浅溃疡型病变。CT扫描病例中,10例有骨质侵犯,CT扫描准确评价8例,遗漏2例。MRI扫描14例,均准确评价肿瘤及其与四周结构的关系。结论 CT、MRI对口腔癌原发肿瘤的形态、大小、四周结构侵犯、颈部淋巴结转移的评价有重要价值。对于较小及呈浸润性生长的表浅溃疡型肿瘤,CT扫描尤其是CT平扫难以准确显示,应首选MRI。

Serum estradiol level of rats was detected by means of radioimmunoassay. The positive staining and intensity of lipid in the meibomian gland alveolus was detected by Sudan Ⅲ staining.

实验评估:采用放射免疫分析方法检测血清雌二醇水平;采用组织化学苏丹Ⅲ染色方法对睑板腺腺泡上皮做脂质染色阳性和强度检测。

Results Although overexpression of P53 protein was not detected in normal eyelid cutaneous epithelium, meibomian gland and mild epithelial dysplasia, the positive rate of P53 increased in degree in moderate and severe dysplasia, carcinoma in situ and invasive tumors, and PCNA labeling index progressively increased too. Overexpression of P53 protein was detected in 30/53 cases of basal cell carcinoma, 16/32 cases of squamous cell carcinoma, 10/17 cases of meibomian gland carcinoma, and the intensity of P53 protein immunostaining increased with the lowering in the degree of histological differentiation.

结果 在正常眼睑皮肤、睑板腺及轻度不典型增生眼睑上皮中,未见P53蛋白表达,而中、重度不典型增生,原位癌,浸润癌中,P53蛋白阳性表达率逐渐增高,其 PCNA指数也有增加趋势。30/53例基底细胞癌(basal cell carcinoma,BCC)、16/32例鳞状细胞癌(squamous cell carcinoma,SCC)、10/17例睑板腺癌中,P53蛋白阳性表达;同时,随着肿瘤组织分化程度的降低,P53蛋白阳性表达程度有逐渐增高的趋势。

Some field samples of Solanaceae collected from Zhejiang and Shanxi Provinces were detected by DAS-ELISA and biological tests, results showed that ToMV was a common pathogen of tomato and eggplant. About 50%of detected Solanum melongena samples had ToMV both in Zhejiang and Shanxi provinces.However,there was a significant difference between tomato samples from Zhejiang and Shanxi provinces.In Zhejiang province, only about 10%of samples were infected by ToMV, while the numbers of infected samples were above 60%in Shanxi.

利用此检测系统并结合生物学方法,对浙江及山西等地的田间病样进行测定,结果发现茄子、番茄等茄科作物中均有ToMV的侵染,其中茄子中有50%左右的发生率,番茄中ToMV发生率南北差异较大,在浙江发生率仅为10%左右,而在山西有60.7%的发生率,辣椒中未测到ToMV。

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