查询词典 detected

In Western blotting, Ig and Ig heavy chain of the sturgeons have the same antigenicity and can be detected by antibody of rabbit anti the sturgeon Ig respectively in colloxylin membrane while light chain cannot be detected.

Western blotting的检测结果表明，3种鲟鱼Ig的重链与其Ig具有同样的抗原性，在硝酸纤维素膜上可被兔抗鲟Ig多克隆抗体所识别，而轻链的Western blotting检测结果则呈阴性。

The surface of image can be looked as a curved surface, and we can simulate a conicoid function in local area. Based on this function we can estimate the gradient and the curvature of the curved surface points, and set proper thresholds of gradient and curvature, we can detect the centre lines of roads with correlative process including removing small areas, area growth, thinning, etc. Lastly through seting proper thresholds of the gradient and the curvature again, the original road junctions can be detected and then verified by the road lines detected formerly.

基于微分几何的道路交叉口识别方法：把影像的灰度表面看作是一个曲面，在局部区域内拟合一个二次曲面函数，通过该函数来估算梯度和曲率并设定合适的梯度和曲率阈值来检测道路中心线，并进行相关处理，例如去除小区域、区域增长、细化等，然后再设定合适的梯度和曲率阈值，检测出初始道路交叉口，并用检测出来的道路来进行确认。

In addition, a benzene, toluene (0.32%), was also detected in 80% ripening fruit. In 90% ripening fruit were detected 27 kinds of aroma components belonging to 2 categories. Among the components, 17 were esters, and 10 alkenes. Of the 17 esters, methyl octanoate is the highest (21.00%), followed by ethyl hexanoate (17.64%), methyl hexanoate (14.34%). Of the 10 alkenes cis-β-ocimene is the highest (1.00%), followed by à-copaene (0.81%) and trans-ocimene (0.48%).

在9成熟果实中检测到2类共27种香气成分，分别是酯类17种和烯类10种；在酯类中，相对含量最高的是辛酸甲酯(25.27%)，其次是辛酸乙酯(21.00%)、己酸乙酯(17.64%)、己酸甲酯(14.34%)；在烯类中相对含量较高的有β-顺式－罗勒烯(1.00%)、à-蒎烯(0.81%)和反式－罗勒烯(0.48%)。

In this study, copepoda and its resting eggs in shrimp culture ponds were detected using PCR dot-blot hybridization and nest-PCR for the presence of WSSV. The results showed that WSSV positive cases were detected in copepoda from egg to adult, and the positive copepoda appeared earlier than cultural shrimp in ponds.

1。本研究利用PCR-斑点杂交和巢式PCR法检测野外发病对虾养殖池塘中桡足类及其休眠卵，以及由这些休眠卵孵化出的幼体携带病毒情况，发现桡足类由卵到成体各阶段中均有阳性个体，而且池塘中在春天放养对虾苗前桡足类就出现阳性个体。

METHODS: The BEC were preincubated with crocetin (1, 0.1 and 0.01 μmol/L) for 12 h, then exposed to AGE (100 mg/L). The RAGE mRNA expression was detected by RT-PCR analysis, the intercellular adhesion molecule-1(ICAM-1) was measured by ELISA. The extracellular superoxide ion and thiobarbituric acid reactive substances were assessed with superoxide ion kit and colorimetric assay, respectively The intracellular I2O2 was also detected using the probe 2,7-dichlorofluorescein. The mitochondrial membrane potential and mitochondrial Succinate dehydrogenase were analyzed by the retention of rhodamine 123 (Rh123) and MTT.

不同浓度的西红花酸(1、0.1、0.01μmol/L)预孵BEC细胞12h后，用AGE(100mg/L)刺激细胞12h，RT-PCR法测定RAGEmRNA的表达水平；ELISA法测定细胞间黏附分子－(ICAM-1)的表达；试剂盒分别检测胞外超氧阴离子和硫代巴比妥酸反应产物浓度；同时，还用2，7-二氯荧光素测定了胞内H2O2的浓度，并用罗丹明123(Rh123)荧光法及MTT法分别检测细胞线粒体膜电位水平和其琥珀酸脱氢酶的活性。

Methods The Cynomorium polysaccharide and Cynomorium water extract were extracted and prepared chewable tablets. Wistar rats were randomly divided into normal control, aging model, VitE, Cynomorium polysaccharide chewable tablets and Cynomorium water extract chewable tablets groups. Dgalactose was injected to establish the aging model. After drug intervention, the proliferation activity of rat spleen lymphocytes stimulated by ConA was detected by MTT assay and the spleen index was measured. The pathological morphological changes of spleen were observed. The activity of superoxide dismutase, the content of malondialdehyde and nitric oxide in serum of rats were detected.

提取并制备锁阳多糖、水提取物咀嚼片；Wistar大鼠随机分为空白对照组、衰老模型组、维生素E对照组、锁阳多糖组、水提物组，以D半乳糖建立衰老大鼠模型，药物干预后，MTT法检测各组大鼠脾淋巴细胞对ConA刺激的增殖能力，检测各组大鼠脾脏指数，观察脾组织的病理形态学变化；并检测各组大鼠血清中超氧化物歧化酶活性、丙二醛及一氧化氮的含量。

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果：1合成的CS-PAA磁性聚合物微球呈球形，大小均一，TEM测定其干态下粒径为80nm左右，磁化曲线证实具有超顺磁性，具有一定的PH敏感性，固载表柔比星后，水溶液性状稳定，无沉淀物，DLS测定直径约200nm左右，测定载药率为15％，较文献报道高，包封率为20％。2肉眼观察试验组大鼠膀胱表面呈褐色，可见大量的Fe粒子聚集，普鲁士兰染色法显示，试验组大鼠膀胱壁内有大量的Fe粒子，分布至膀胱壁全层，与对照组大鼠相比，试验组大鼠的肝、脾内的Fe粒子聚集量明显降低；HPLC测定结果与Fe染色相同；高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高，其他组大鼠血生化指标没有明显变化。3MTT法发现，高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组，FCM发现试验药物组可引起明显的肿瘤细胞凋亡，试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C，试验组细胞Bcl-2蛋白减少，Bax蛋白变化不明显，Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P＜0.01)，其中高剂量组的抑制率最高。

A large number of digenic interractions were detected, 18.2% of which were detected simultaneously in both two years.

在检测到的大量2位点互作对中，约18.2%在两年都能被检测到。

Four fever' patients were detected,the incidence of endogenous malaria was 0,1 335 flowing population and returners from other places were detected,3 ecdemic patients were found out,the positive rate was 0.22%.

全市共血检&四热&病人11 018人，疟疾内源性发病率为0。流动人口和当地居民外出回归者血检1 335人次，输入性疟疾病人3例，阳性率为0.22%。

The results dicate that there was nearly no difference from hibernation and activity period in all tissures except the liver and skeletal muscle of Elaphe rufodorsata as shown by electrophoresis plot, hibernation was lower than in activity period except that in liver of Erinaceus dealbatus, and LDH4 was not detected in all detected tissues of the three species of snakes.

结果表明：LDH同工酶的电泳图谱只有红点锦蛇的肝和骨骼肌中存在活动期和冬眠期的明显差异，在其余动物两个时期的组织中未表现出明显变化；刺猬的肝脏LDH活性在冬眠期显著地高于活动期，而其余的动物组织冬眠期LDH活性均显著低于活动期；三种蛇类组织LDH4均未见表达。

It seems likely that Kraft will return with a better offer.

看起来卡夫要重新考虑一个更好的出价了。

But he'd rather burn off my feelers and watch me squirm.

可他却放任那个家伙对我为所欲为！