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1.To study the expression of in the different glucose concentration with the time changing. 2.To study the effect of different concentration of Hepatocyte Growth Factor on the Integrin-linked kinase in protein and mRNA level by Mesangial Cells in high concentration (25 mmol/L) of glucose. 3.To study the effect of different concentration of HGF on the Fibronectin'secretary by Mesangial Cells. 4.To study latent therapeutic action and possible mechanism of HGF on the glomeruler sclerosis. The difference of ILK's experession on mRNA level in different concentration of glucose and HGF are detected by means of revers transcription-polymerase chain reaction,and by means of immunohistochemistry to detect their difference in protein level;the effection of HGF to the FN's secretary in high glucose are detected by means of enzyme linked innunosorbent assay.

1探讨在不同的血糖浓度下大鼠肾小球系膜细胞中ILK伴随时间变化在蛋白和mRNA水平表达的差异 2探讨不同浓度的HGF对高糖环境下(25mmol/L)大鼠肾小球系膜细胞中ILK在蛋白和mRNA水平表达的影响 3探讨不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响 4初步探讨HGF对肾小球硬化的潜在的治疗作用及其可能机制采用RT-PCR的方法分析在不同血糖浓度和不同HGF浓度下大鼠肾小球系膜细胞中ILK在mRNA水平表达的差异；采用免疫组织化学的方法直观的观察它们在蛋白水平表达的差异；采用ELISA法分析不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响。

This invention concerns a current control process for a feed-in voltage converter, wherein a detected flux-inducing current component and a detected torque-inducing currrent component of an actual currrent vector of a rotating field machine are each controlled for a flux-inducing current component set value and a torque-inducing current component set value of the rotating field machine. To that effect, a control set-value (DELTA u*sp, DELTA u*sq) is determined, dependent each time upon a determined current component control deviation. From these, current components of an actual current vector are formed each time by overlay of a formed pilot value (u*spvor, u*sqvor). This invention also concerns a device for carrying out this process.

本发明涉及一种用于馈入电压式变流器的电流调节方法，其中分别将所检测的一感应式电机的一实际电流向量（i s 的磁通感应电流分量和一转矩感应电流分量i sq ）调节至该感应式电机的一额定电流向量的一磁通感应分量额定值和一转矩感应电流分量额定值，在这种情况下，分别根据所检测的一电流分量调节偏差计算出一调节器调节量（△u * sp ，△u * sq ），由这两个调节器调节量分别通过叠加所形成的一预控制量（u * spvor ，u * sqvor ）形成一额定电压向量的电压分量（u * sp ，u * sq ），本发明还涉及一种用于实施该方法的装置。

Activity of HO and concentration of H2S in plasma are detected by Spectrophotometer, concentration of hydrocortisone is detected by Electrochemiluminescence immunoassay,compared with 28 control subjectives.Drug action on the activity of HO、the concentration of H2S and hydrocortisone for schizophrene is observed.

采用分光光度计法测定其血浆内HO活性和H2S含量，采用电化学发光免疫分析法(Electrochemiluminescenceimmunoassay，ECLIA)检测血浆皮质醇水平，并与28名健康者对照比较，观察利培酮对精神分裂症患者血浆HO活性、H2S和皮质醇含量的影响。

Sometimes non cancerous polyps can contribute to colon cancer and these can be detected by a sigmoidoscopy which can detect sigmoid colon cancer or a colonoscopy and they can be detected very early.

有时非癌性息肉可有助于结肠癌和这些可以侦测由sigmoidoscopy ，其中可以侦测到乙状结肠癌，或大肠镜检查，他们可以很早就发现。

Concentrations of DA and 5-HT in different regions were measured by high performance liquid chromatography; the expression of SERTmRNA was detected by in situ hybridization; the degeneration of neuron and terminal was demonstrated by Bielschowsky and Glee Marsland silver staining; and the protein expression of GFAP was detected by immunohistochemistry.

2采用高效液相色谱法测定不同脑区神经递质DA和5-HT的含量；采用原位杂交方法检测SERTmRNA；Bielschowsky及Glee Marsland氏改良银浸染法显示神经元胞体、树突和轴突；免疫组织化学方法检测GFAP的表达。

Methods The lymphocytes of rats were isolated from the peripheral blood and cultured with sodium arsenite under 37 ℃.The ROS content was detected by DCFH-DA;The content of LPO was detected by fluoresce method.

体外分离大鼠外周血淋巴细胞后，施加处理因素，在37℃条件下恒温培养，用2'7'-二乙酰二氯荧光素染色法检测细胞内的ROS水平，用硫代巴比妥酸荧光法测定细胞内LPO含量。

Methods: hypoxia model of detrusor smooth muscle cell in vitro was established by sodium hyposulfite which consumed the oxygen of the medium. the cx43 and cx43 mrna were detected by western blot and rt_pcr. the level of malondialdehyde was detected.

体外培养膀胱逼尿肌细胞，用连二亚硫酸钠清除培养基内的氧，建立缺氧模型，通过rt_pcr、western blot检测正常对照组、缺氧组及vite预处理组的逼尿肌cx43表达情况，并检测丙二醛质量浓度。

Immunohistochemistry for HIF-1α: HIF-1αexpression was detected in germ cell cytoplasm cytoplasm, obviously spermatocyte,and was detected in nucleus of seminiferous tubule theca cell too.

免疫组化观察HIF-1α的表达情况： HIF-1α表达主要见于生精细胞的细胞质，以精母细胞明显，也见于曲细精管外周膜细胞的胞核。

The results showed that, under P sufficient conditions, 2 QTL were identified for SPUE on chromosome 1B and 5A, respectively; 3 QTL were detected on chromosome 2B, 5A and 7A, respectively, influencing WPUE. Under P deficient conditions, 3 QTL on chromosome 2D, 3B, and 6D were detected for SPUE, and 2 QTL on chromosome 2D and 7A for WPUE.

结果表明，正常供磷，有2个与SPUE有关的QTL，分别位於染色体1B和5A上，变异解释分别为6.55%和11.61%；与WPUE有关的QTL有位於染色体2B、5A和7A上的3个；SPUE和WPUE还分别受一对互作位点的影响。

We detected that EGF mRNA was expressed sflungly lii the oocyte, and is also found hi gmnulosa cells, the cell fium smaller foflicular expressed stronger than fium bigger one. In the corpus hemonbaglcwn corpus luteurn, lean type and pseudocorpus-luteum, EGF rnRNA was detected,, no distinct difference can be seen in them. The EGF mRNA expressed strongly in fimbria end, ampulla and isthmus of oviduct, in the big follicular stage, ovulation stage, pregnancy stage and spurius pregnancy stage, we can not see any distinct change in them, but hi the medium follicuar stage,it is weaker.

结果发现：猪卵母细胞中EGF的mRNA强烈表达，且小卵泡卵母细胞→中卵泡卵母细胞→大卵泡卵母细胞中，EGF的mRNA表达量有逐渐减少的趋势；猪卵泡的颗粒细胞中有EGF的mRNA表达，小卵泡颗粒细胞→中卵泡颗粒细胞→大卵泡颗粒细胞中，EGF的mRNA表达也有逐渐减少的趋势；猪卵巢中的红体、黄体、白体和假黄体中都有EGF的mRNA表达，看不出几部分的表达量有明显的强弱变化；猪输卵管伞部、壶腹部和峡部，都有EGF的mRNA表达，在大卵泡期，排卵期，孕期和假孕期都强烈表达，各期间看不出明显的强弱变化，中卵泡期表达较弱；猪子宫中EGF的mRNA在大卵泡期，排卵期，孕期和假孕期都强烈表达，看不出表达量的明显变化，而小卵泡期表达量明显减弱。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。