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The protocol for the detections of all the AIV isolates, the H5-subtype and the optimization of reactions were studied with a H5-subtype reference isolate BSG1. A 229bp-band of AIV specific and a 380bp-band of H5 subtype specific were amplified individually with the designed primers of AIV specific and H5-subtype specific respectively by the developed RT-PCR technique from isolate BSG1. A double-PCR was also developed which can detect all the AIVs and identify the H5-subtype AIVs. The results of specificity experiment showed that there was no specific band could be amplified with the samples of Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, egg drop syndrome virus and infectious bursal disease virus.

结果应用通用引物可从BSG1毒株扩增到与预计大小相符的229bp AIV特异性片段,应用H5亚型鉴定引物则可从BSG1毒株扩增到与预计大小相符的380bp H5亚型特异性片段;在此基础上建立了可检测所有AIV毒株以及可同时进行H5亚型毒株鉴定的二重RT-PCR方法;检测方法的特异性试验结果表明,该方法对新城疫病毒、传染性支气管炎病毒、传染性喉气管炎病毒、产蛋下降综合症、传染性法氏囊病毒无交叉反应;检测方法的敏感性试验结果表明,通用引物扩增、H5亚型特异性引物扩增以及二重PCR扩增反应的组织样品总RNA的最低检出量分别为0.0217μg、0.0217μg和2.17μg;对20份临床样品检测中,有10份为AIV阳性,其中有5份为H5亚型病毒阳性。

Objective To detect the treatment effect and protecting action of photodynamic therapy to early carcinoma of larynx.

目的 探讨光动力治疗对早期喉癌的治疗效果与喉功能的保护作用。

I couldn't detect any significant latency.

我没有发现任何明显的迟滞。

Collins says she suspects that the other five people also had latently infected HPCs, but the test was not sensitive enough to detect the virus because it infects few cells and they only had small samples from each person.

科林斯说,她估计其他五名感染者也可能存在收隐性感染的HPCs,但实验犹豫敏感度不够无法检测到病毒,因为它只感染了极少的细胞,并且从每个人身上获取的样本也很少。

The distribution of microhardness is not uniform across the anodic film for the most specimens and the general trend is that the value of the hardness is higher both in the outer film adjacent to the surface and in the inner film near the interface between film and substrate for the specimens with type Ⅱ and type Ⅲ anodizing technology. The measured modulus for anodic film with type Ⅲ anodized is 80.77Gpa. The thermal expansion coefficients of anodic films are measured by two different methods based on X-ray diffraction technique. One way is to detect the lattice spacing change of crystalline phase in powder specimen of anodic film as the increment of temperature by powders of anodic film; and the other way is to determine the thermal stress by entire specimen, that is, the anodic film and the substrate attached together.

利用X射线衍射的方法采用两种不同手段对氧化膜的热膨胀系数进行测试,一种方法为采用氧化膜粉末样品,测量温度变化过程中晶面面间距的改变,进而推出其热膨胀系数;另一种方法为整体试样测试氧化膜中的热应力,通过热应力和热膨胀系数之间的关系计算氧化膜的热膨胀系数。

The team said it hoped to bring a simplified kit to market that could be used by the layperson, at home or in the field, to detect melamine in food.

研究组希望通过简化产品形式投入市场,如此,无论是在家或在行业里,外行也能简便操作检测食品中三聚氰胺。

Parents might suspect the child is not developing normally, but there are hidden factors a parent can't detect, like the lazy eye that is steadily growing weaker, said Dr.

父母亲可能会感到孩子的发育不正常,但是有一些隐藏的因素他们无法觉察到,像弱视这种情况一般是逐步加重的。

However, when the skew between input data and the input clock is close to half step time, the "three times oversampling" system can not detect whether the skew leads or lags, and may induce errors in recovered data.

当输入资料偏移量接近二分之一步距时,三倍取样架构将无法分辨出偏移量是领先还是落后取样时脉,因此可能造成恢复资料的出错。

This system includes the leakage current detect unit and the remote computer control and administer center.

该系统包括漏电监测单元和远端计算机管理控制中心两部分。

Used to detect memory leakage problems, it is a rather good one source!

用于检测内存的泄漏问题,它是比较好的源码之一!

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推荐网络例句

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。