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Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在囊胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Where cleft palate occurs by itself, it is not possible to detect it before birth.

如腭裂发生的本身，是不可能的检测，它在出生之前。

This study investigated the ant groups algorithm to solve the problem of traveling salesman. The target of the article is that on the map G detect the shortest length of the Hamilton Circle, namely one pair of C n cities and only visit one of the shortest closed curve.

论文主要是研究蚁群算法来解决旅游商问题，其目标是从无向图G中寻出长度最短的Hamilton 圈，此即一条对C中n个城市访问且只访问一次的最短封闭曲线。

At the same time, the minimal area term has the global optimize characteristic, which can detect all the contours inside the object with one initial closed curve.

同时，该约束项具有全局优化的特点，仅需要一条初始闭合曲线，就可以将内部带空洞目标的内外边缘全部检测出来。

Immunohistochemical staining was used to detect the protein levels of Bcl-2 and Bax in the cochlea after 3 months.

在成模后3个月时采用免疫组织化学方法，对大鼠耳蜗组织进行Bcl-2和Bax的表达检测。

Methods immunohistochemstry method was used to detect the locations of nos and anp in the mammalian cochlear different cells.

采用免疫组织化学双标法检测anp和nos在正常豚鼠耳蜗的分布特征。

In addition, using RT-PCR to detect expression of centrin homogene during Urechis unicinctus spermatid deferentiation and early embryonic development. The result revealed that (1) expression of HsCEN1 homogene is present during spermatid defferentiation including spermtid and sperm, but not in adult tissues including body wall tissue,intestinal mucous memberane cells and cell in coelom succus;(2) expression of HsCEN1 homogene is not present in unfertilized oocytes but present in that of released polar bodies after fertilization and parthenogenesis oocytes treated with A23187;(3) expression of HsCEN1 homogene is present during early embryonic development including two-cell stage,multicell stage,gastrula to trochophora.

我们还用RT-PCR方法检测了Centrin同源基因在单环刺螠精子分化过程和早期胚胎发育过程的表达，结果显示：在单环刺螠精子发生过程中的精细胞、精子团、精子中都有表达，而在成体的体壁组织、消化道组织、体腔球中则没有表达；在未受精卵中没有表达，而在受精后释放极体的受精卵和经A23187孤雌激活的卵中则有表达；在单环刺螠的早期发育过程——二细胞胚胎、多细胞胚胎、原肠胚、早期担轮幼虫——中都有表达。

In the state of cold boot, CPU will detect automatically whether the EEPROM has been replaced by a new one (may substitute it when being maintained). If CPU judges that the EEPROM is a new one or there are some errors on the data, it will automatically read in the initialized data so as to ensure the

在冷开机时，CPU会自动检测是否更换了新的EEPROM，如果CPU判断EEPROM是新更换或数据出错的，为了保证机器的工作，CPU会自动读入初始化数据，这时电视机总线控制将按初始化状态正常工作。

A rapid, efficient protocol to detect the toxicity of Cu2+ stress using wheat coleoptile, which is simple, uniformal in structure and sensitive, was established. By using this model, the mechanism of Cu2+ stress leading to the inhibition of cell elongation was also studied.

本文以小麦为材料，研究不同浓度Cu2+对小麦种子萌发和幼苗生长的影响及机理；利用小麦胚芽鞘结构简单、反应敏感的特点，探索建立小麦胚芽鞘快速检测铜离子毒害模式，并利用此模式研究了铜离子胁迫对细胞伸长抑制作用的机理。

This time he thought he could detect colicky symptoms, and he began to encourage them with considerable hope.

他又查找了一番，这次他以为可以找出肚子疼的理由，并且满心希望地让疼痛发作。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新