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Fluorescein isothiocyanate labeled polyclonal goat antihuman immunoglobulin antibody was added, and flow cytometer was used to detect bead-platelet-associated autoantibodies-antihuman immunoglobulin antibody complex. Results The fluorescene ratio of four monoclonal antibodies was significantly different (P.01) between the idiopathic thrombocytopenic purpura patients and either the non-ITP patients or the normal controls. If the upper limit of normal control was set as cutoff value, ratios of greater than 1.37, 1.24, 1.48 and 1.19 were considered positive for the four monoclonal antibodies respectively. The flow cytometric bead assay had an overall sensitivity of 73.17% and a specificity of 94.29%. The overall sensitivity was significantly higher (P.05) than that of modified indirect MAIPA and that of using single antibody.

结果 特发性血小板减少性紫痕组4种羊抗荧光强度比值与非ITP血小板减少组和正常对照组有显著性差异(P.01);若将ITP组患者4种羊抗荧光强度比值分别大于正常对照组上限1.37、1.24、1.48和1.19判断为阳性,则流式微球技术检测血小板特异性自身抗体的敏感性为73.2%,特异性为94.3%;4种羊抗联合检测总体敏感性明显高于改良间接单抗特异的血小板抗原固定试验(P.05),且大于各单个抗体检测敏感性。

Fluorescein isothiocyanate labeled polyclonal goat antihuman immunoglobulin antibody was added, and flow cytometer was used to detect bead-platelet-associated autoantibodies-antihuman immunoglobulin antibody complex. Results The fluorescene ratio of four monoclonal antibodies was significantly different (P .01) between the idiopathic thrombocytopenic purpura patients and either the non-ITP patients or the normal controls. If the upper limit of normal control was set as cutoff value, ratios of greater than 1.37, 1.24, 1.48, and 1.19 were considered positive for the four monoclonal antibodies respectively. The flow cytometric bead assay had an overall sensitivity of 73.17% and a specificity of 94.29%.

结果 特发性血小板减少性紫癜组4种单抗荧光强度比值与非ITP血小板减少组和正常对照组有显著性差异(P<0.01);若将ITP组患者4种单抗荧光强度比值分别大于正常对照组上限1.37、1.24、1.48和1.19判断为阳性,则流式微球技术检测血小板特异性自身抗体的敏感性为73.17%,特异性为94.3%;4种单抗联合检测总体敏感性明显高于改良间接单抗特异的血小板抗原固定试验(P<0.05,且大于各单个抗体检测敏感性。

More than 200 subatomic particles have been detect ed so far, and most appear to have a corresponding antiparticle.

至今已经检测到200多种亚原子粒子,大多数都有相应的反粒子。

Immunochemistry analysis showed that the antiserum could detect the antigen as low as 10 ng.

经ELISA检测该免疫血清的滴度为12 800。

ChinaObjective:To study the antitoxic role of vesicular monoamine transporter 2 (VMAT2) in transpgenic Chinese Hamster ovary cell.Methods:With the technology of transgene from PC12 to CHO,MTT reduction assay was used to detect MPP^+ toxic effect on wild type CHO and transgenic CHO.

利用转PC12细胞基因到CHO细胞中形成的转基因CHO细胞(cDNACHO,采用MTT比色法检测1-甲基-4-苯基吡啶离子(MPP^+对CHO细胞野生株(wtCHO和cDNACHO细胞的毒性作用,并观察利血平-VMAT2的特异性阻滞剂对MPP^+毒性作用的影响。

Then, in situ hybridization was performed to detect expression of GHR in the antler tip.

成功克隆到了1 967 bp的序列(GenBank登陆号为EU381142),该序列包含GHR基因的全部编码序列。

I am 29 years old, March 22 last menstruation, April 2 to detect ovulation, it sports a bit, after a cold, fever, and sore throat, aphonia, sub gum pain, always thought that was lit, it dared not take medicine, drink plenty of water, then April 8 daily early pregnancy test, then there is no situation (I thought that implantation may be able to test out), I may want to this gone, eat a piece Yinqiaojiedu, one of glycyrrhiza one Banlangen Chongji, April 14-16 one day into the night on the bad cough, could not sleep.

我今年29岁,3月22日末次月经,4月2日测出排卵,所以运动了一下,之后就感冒了,发烧了,嗓子疼,失音,牙床子疼,一直以为是上火了,也没敢吃药,多喝水,后来4月8日用早早孕测了一下,没有状况(我以为着床可能就能测出来),我想可能这次没戏,就吃了一次银翘解毒片,一次甘草片,一次板蓝根冲剂,4月14-16日一到晚上就咳得厉害,睡不着觉。

230 That one may detect a dynastic purpose in the book and that its author was a Sadducean apologist for the Hasmoneans.

,一个可探测王朝在这本书的目的,其作者是Sadducean代言人的哈斯摩年王朝。

In addition, the ability of the copolymer to detect different metal cations in aqueous solution was investigated.

研究了常见金属离子对聚合物荧光强度的影响,其中以Fe~(3+)对聚合物的荧光淬灭程度最大。

We use proteomic approach:two-dimensional electrophoresis and mass spectrometry to detect the proteins expression changes modulated by areca nut extracttreatment and attempted to link the correlation between OSCC and areca use.

本研究使用蛋白体学研究方法――二维电泳搭配质谱仪分析法,探讨口腔细胞受槟榔萃取物影响之蛋白质表现的改变,期望藉由高产能的策略探讨槟榔与口腔癌间的相关性。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。