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Flow cytometry and immunofluorescence were applied to detect the expression of surface markers of amniotic fluid stem cells.

流式细胞仪及免疫荧光法检测羊水干细胞表面标志的表达。

To study the changes of plasma endotoxin NO and hemorheology in experimental cirrhotic rats and their correlation,we have used hemorheology analysis system and the means of limulus amoebocyte lystata assay Griess assay to detect changes of plasma endotoxin NO and hemorheological parameter in rats at 3 6 9 weeks after CCl4 administration.

为探讨肝硬化中血浆内毒素、NO水平的变化及血液流变学特性的改变及其相互关系,我们通过用四氯化碳(carbon tetrachloride,CCl_4)来诱导大鼠肝硬化模型,采用鲎试剂动态浊度法、镉还原比色法、血液流变学分析仪,分别检测了大鼠肝硬化演变过程中早期(诱导第3周末)、中期(诱导第6周末)、晚期(诱导第9周末)的血浆内毒素、NO水平及血液流变学各参数。

In the model of tumor cells metastases with cell line A673, 10〓 tumor cells can be detected after only one ampliation with outer primers, which means we can find 1 tumor cells in 10〓 normal PBMC, and enlarged again with inner primers, 10〓 tumor cells can be detected, which means we can find 1 tumor cell in 10〓 normal PBMC. In the simulative model of tumor cells metastases with RNA, 10〓 tumor cell can be detected that is to say we can find 1 tumor cell in about 10〓 normal PBMC. In all 15 samples of 11 patients, 2 patients'EWS/FLI1 expressions were negative, no evidence of metastases was found followed up by 8 months and 30 months respectively. Semi-quantitative study was used to detect the expression level of EWS/FLI1 mRNA in the other 9 positive patients, and metastases occurred in 6 high-level expression patients in 2-24 months after the operation, no evidence of metastases in the other 3 low-level expression patients up to now.

应用A673细胞系建立的肿瘤转移模型,单独应用外引物进行一次扩增,可以检测到10〓级肿瘤细胞,即可以从10〓个正常细胞中检测到1个肿瘤细胞;而在此基础上再次应用内引物扩增,则可以检测到10〓级肿瘤细胞,即可以从10〓个正常细胞中检测到1个肿瘤细胞;在用RNA模拟的肿瘤细胞转移模型中,两次扩增可以检测到10〓级肿瘤细胞,即可以从10〓个正常细胞中检测到1个肿瘤细胞。11例患者的15份标本中,2例没有检测到EWS/FLI1 mRNA的表达,分别随访8个月和30个月,没有发现临床转移的证据;对其余9例阳性表达的病例进行半定量的研究,6例EWS/FLI1 mRNA高表达的病例,分别在术后2-24个月发生转移,而3例EWS/FLI1 mRNA低表达的病例,则至今没有发现转移的证据。

It is mainly used to sterilize and detect leakage for ampoule, oral medicine, small infusion bottle, etc.

它主要用于探测和消毒安瓿泄漏、口腔医学、小输液瓶、它设计等先进、结构合理、高档控制。

Using immunohistochemistry and western blotting, to detect the expression of glucocorticoid receptor in the central amygdaloid nucleus of respective rat groups, and image analysis and statistical analysis were performed.

应用免疫组化、Western Blotting定位定量检测各组CeA的GR表达,进行图像分析和统计学处理。

A large, shallow dish is preferred, but its size and weight are limited when an analytical balance is chosen to detect small weight changes.

首选透湿杯是大而浅的杯子,但是选定了用于检测微小重量变化的分析天平后它的尺寸和重量也就受到了一定的限制。

Sum up as follows now. This group uses 1 clinical data ancon vein is pressed determine heart function of the patient 44, male 24, female 20; age 46 years old of ~79 year old, average 56 years old of; classification by function of new York heart, heart failure Ⅲ spends 6,Ⅱ spends 23,Ⅰ spends 15; right heart failure 31, complete heart failure heart disease of sex of source of 13; lung 14, hypertensive heart disease 8, coronary heart disease 5 (among them acute cardiac muscle straightens dead 2), outspread sex cardiac muscle is ill 7, rheumatism sex heart disease 4, hypertensive heart disease is amalgamative coronary heart disease 3, be short of disease of cardiac muscle of courage and uprightness 2, congenital heart disease 1. 2 detect 2.1 equipment treat the method dish 1, l measure press glass to be in charge of 1 (long 30cm, internal diameter 4mm), also can use measure a head to press a canal to replace, 10mL injector 1, 12500U heparin fluid 1. 2.2 operations patient is taken smooth lie, make be exhibited outside the upper arm show ° of 45 °~60 with trunk, and difficulty breathes like the patient in same horizontal; with right atrium, desirable lie partly, arm height is equivalent to the 2nd costal region.

现总结如下。1临床资料本组采用肘静脉压测定病人的心功能44例,男24例,女20例;年龄46岁~79岁,平均56岁;按纽约心功能分级,心力衰竭Ⅲ度6例,Ⅱ度23例,Ⅰ度15例;右心力衰竭31例,全心力衰竭13例;肺源性心脏病14例,高血压心脏病8例,冠心病5例(其中急性心肌梗死2例),扩张性心肌病7例,风湿性心脏病4例,高血压心脏病合并冠心病3例,缺血性心肌病2例,先天性心脏病1例。2检测方法2.1设备治疗盘1个,L型测压玻璃管1个(长30cm,内径4mm),也可以用测脑压管代替,10mL注射器1具,12500U肝素液1支。2.2操作病人取平卧位,使上臂外展和躯干呈45°~60°,且和右心房在同一水平面;如病人呼吸困难,可取半卧位,手臂高度相当于第2肋间。

FISH can detect aneuploid malignant cells in sputum and be used as a complementary technique to cytologic diagnosis of lung cancer.

FISH不仅可以检测痰标本中的异倍体肿瘤细胞,而且也可以作为痰细胞学诊断的辅助技术。

Feature of principal lines spatial distribution: detect the principal lines through edge detecting technology, and then divide the image of palmprint into blocks with same dimension, compute the mean and variance as feature; Feature of palmprint direction: divide the image into sub-images; compute the max angle of projection and coordinate of each sub-image, which stand for the direction and location of the line on the palm; Feature of moment invariants: compute the moment invariants of each sub-image and the whole image as feature.

掌纹主线空间分布特征:利用边缘检测技术检测出掌纹的主线,将图像分割成相同大小互不重叠的子图,提取每一个子图的均值和方差作为特征;掌纹线方向特征:将图像分成不重叠的子图,利用Radon变换求出每一个子图的最大投影角度和坐标,也就是子图内掌纹线的方向和位置,构造特征向量;掌纹不变矩特征:提取图像整体和分割后的子图的不变矩作为特征。

The principle,construction of the vibrating gyro with accelerometers are described,which can detect linear acceleration and angular velocity simutaneously.

叙述了能同时敏感运动物体线加速度和角速率的加速度计陀螺的原理、结构及微机械加速度计陀螺的性能。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。