查询词典 detect

Methods Purified 5-LO fragment was injected into male pronucli and the firtilized eggs were transplanted into pseudopregnant mice. PCR and Southern blot were used to detect the genotype of DNA separated from the newborn mouse tail tissues. RT-PCR and Western blot analysis were used to detect the gene transcription and expression. Results PCR and Southern blot results showed that 7 of 25 mice were transgenic mice.

通过显微注射的方法，将5-脂氧化酶基因片段(6.8 kb)导入BDF1受精卵雄原核并移植到同期受孕的假孕母鼠输卵管中，对产出仔鼠的鼠尾组织DNA进行PCR、Southern blot检测，对9、20、24号转基因小鼠分别提取腹腔细胞、骨髓细胞及脾、肾组织总RNA和蛋白，并采用RT-PCR、Western blot方法进行转录水平检测和蛋白表达检测。

Methods: To create the model of myocardial infarction, to detect the serum SOD and LPO of the MI rats by the pyrogallic acid self-oxidation and detect myocardium tissue ROS of the MI rats by flow cytometry.

建立IR心肌梗死动物模型，采用邻苯三酚自氧化法检测梗死大鼠血清超氧化物歧化酶、过氧化脂质；运用流式细胞术检测梗死大鼠心肌组织中性粒细胞活性氧。

The main conclusions are: Mexican hat wavelet is anisotropic one, and it could detect pointlike singularities very efficiently. Morlet and Cauchy wavelet could be used to detect specific directions, and Cauchy wavelet has better direction selectivity than Morlet one. And it has been used to separate the wave field of Ricker. Its results are basically accurate and computation is more efficient.

主要结论有：Mexcian帽小波是各向同性的，主要用来检测点状信号分量，Morlet小波和Cauchy小波主要用来检测方向信号分量，并且Cauchy小波比Morlet小波的方向性要好，检测结果的分辨率要高；利用二维连续小波反变换，以合成的Ricker子波为模拟信号为例进行波场分离，提高了运算效率，实验结果基本上比较准确，还需要实际地震信号的进一步验证。

A complete bovine 18ku-bFGF gene was cloned by nested-PCR and subcloned into expression vector pET-28a.The recombinant plasmid pET-28a-bFGF was transformed into Escherichia coli BL21.At 25℃,recombinant protein was induced by 0.5mmol/L IPTG for 5 hours.Supernatant of cell lysate was purified using Ni-NTA method and the products was submitted to detect the bioactivity by Western-blotting,which indicated that the fusion protein contained recombinant bovine bFGF.Results showed that recombinant bovine bFGF was produced and solubly existed in the supernatant.NIH-3T3 fibroblasts were used to detect the bioactivity of the fusion protein.

采用巢式PCR方法克隆了牛18ku-bFGF基因完整的编码序列，并构建了原核表达载体pET-28a-bFGF，将其转化大肠杆菌BL21，在25℃低温条件下，用0.5mmol/L IPTG诱导表达5h，用Ni-NTA亲和纯化细胞裂解上清液，经Western-blotting检测，结果显示，在特定的诱导条件下，重组牛bFGF基因在大肠杆菌中获得了表达，并且主要以可溶性状态存在于细胞中。

The fiber-optic DNA biosensor array can be used to detect a specifical gene or detect multi-DNA sequences simultaneously.

这种制备光纤DNA传感器阵列的方法可用于中、低密度的基因芯片的生产。

In order to detect the interested boundary fast and correctly, an boundary point tracking algorithm was proposed to detect the specifical edge of the image.

为了快速准确的检测特定边界，采用边界点跟踪算法检测特定轮廓边界点。

Naphthylamide heavy nitrogen spectrophotography was adopted to detect the content of nitric oxide in the serum and half quantitative reverse transcription polymerase chain reaction was used to detect eNOS gene expression in artery vessels.

采用萘胺重氮分光光度法测定血清NO含量，采用半定量RT PCR方法检测各组动物动脉壁匀浆中eNOS基因的表达，分析造模及各药物组血清NO含量及eNOS基因表达的变化。

Methods: Blood samples were taken from 40 RRTI children (26 males and 14 females, mean age of 3.68±1.47 years) and 35 normal children. Enzyme linked immunosorbent assay method was used to detect the levels of serum tIgE and Western blotting method was used to detect positive percentage of sIgE.

RRTI患儿40例，对照组健康儿童35例，采用酶联免疫吸附法检测血清tIgE水平，欧蒙印迹法检测血清吸入与食物组抗原sIgE抗体阳性率。

Methods C3b sensitized yeast cells and C3b unsensitized yeast cells were used to detect C3b receptors and immune complex on red cells,method of xanthine oxidase was applied to detect SOD activity in red cells.

实验利用低碘饲料和碘化钾去离子水复制了不同碘营养状态的大鼠，采用C3b致敏和未致敏的酵母菌及黄嘌呤氧化酶法分别检测红细胞膜上的C3b受体、免疫复合物及红细胞内SOD的活性。

Detect the use of company yarn Uster Switzerland UST4 type device, the technology of yarn indicators Detect sophisticated analysis, the quality of strict Friday, I introduced the company's new 200 Saurer Twister, will become the country's largest-fold twist base.

公司纱线检测采用瑞士UST4型乌斯特仪，对纱线的各项技术指标进行精密的检测分析，质量严格把关五、我公司新引进200台苏拉倍捻机，将成为全国最大的倍捻基地。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。