查询词典 denatured protein

Simultaneously, the total extractible myoglobin decreased and the proportion of metmyoglobin increased significantly when the pressure above 400MPa. Beef discoloration after pressure processing may result from oxidation of ferrous myoglobin to ferric metmyoglobin, globin denaturation and structural changes. At 40℃, the changes of beef color was similar to that of room temperature's. When the temperature rise to 60℃, most of muscle proteins denatured, a small quantity of native myoglobin could not affect the color of beef significantly. Therefore, protein denaturation was attributed to the main cause of discoloration in beef muscle after pressurization at 60℃.

压力处理导致色泽变化的直接原因可能是由于二价铁的肌红蛋白氧化成三价铁的高铁肌红蛋白、球蛋白的变性及结构的变化所致。40℃时的压力处理，色泽的变化规律与室温下相似，只是色泽变化的程度更为剧烈，蛋白质变性在色泽变化中起到的作用逐渐加强。60℃下的压力处理，绝大部分蛋白质发生变性，少量未变性的肌红蛋白对色泽不会产生明显的影响，此温度的压力处理，蛋白质变性是造成肌肉失去红色的主要原因。

To develop standard WNV diagnostic tools, four monoclonal antibodies against WNV envelope protein domain III were produced. The isotype, the reactivity with denatured and native antigens, the affinity and specificity of antibodies have been characterized. Two MAbs (4B3 and 2F5) were used to establish an antigen capture-ELISA detection system.

为了建立有效的WNV检测方法，本研究制备了四株抗WNV囊膜蛋白第三结构域的单克隆抗体，鉴定了抗体的亚型、抗体和变性及非变性抗原结合的强度、抗原和抗体结合的亲和力常数，进行了免疫荧光分析、测定了抗原表位竞争、抗体的中和能力等，并检测了上述单抗与日本乙型脑炎病毒的交叉反应情况。

Sodium lauroyl sarcosine was used as a denaturant, a novel protein refolding method, which was proposed on the basis of one step-AMC and IEC, one step-AMC-IEC was used on the refolding of denatured/reduced lysozyme.

一步人工分子伴侣-离子交换色谱法辅助还原变性溶菌酶的复性：以月桂酰基肌氨酸钠(Sodium Lauroyl Sarcosine，Sarkosyl，SLS)作为蛋白变性剂，发展了一步人工分子伴侣-离子交换色谱法，对还原变性溶菌酶进行了复性研究。

On cooling a solution of denatured BPTI, the activity of the protein is restored.

变性的BPTI溶液冷却时，蛋白质的活性得到恢复。

In recent years, chromatography has been gradually used in protein refolding from denatured state to biologically active conformation.

近年来层析技术的一个拓展是用于辅助蛋白质从变性态恢复到活性态，即所谓的复性，取得了令人瞩目的进展。

The protein purified with Ni-NTA column was denatured by 8 mol/L urea and dialyzed for refolding.

Ni-NTA柱纯化后的特异性蛋白经8 mol/L脲素变性，梯度透析使蛋白质复性。

The aggregation interaction between reduced-denatured egg white lysozymes during refolding procedure in urea solution was studied by means of reducing and non-reducing protein electrophoreses.

通过还原和非还原蛋白电泳，研究了还原变性蛋白溶菌酶在脲溶液中重折叠过程的集聚相互作用。

In contrast, deletion of the propeptide yields mature but inactive NK, the inclusion bodies of NK or denatured rNK couldn't be renatured in any condition, but with the aid of exogenously purified propeptide or Ser221Ala proNK, the inactive mature protein could be renatured by an intermolecular pathway in vitro.

此外，我们利用融合基因表达系统在大肠杆菌中高效表达了谷胱甘肽S-转移酶／NK和硫氧还蛋白／NK两种融合蛋白，经体外复性、纯化，均获得活性蛋白。以上结果显示，pro肽在体内体外，通过分子内或分子间的方式均能帮助NK分子的折叠与再折叠，作为一种分子内的分子伴侣，pro肽为NK分子正确折叠所必需。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失