查询词典 denaturalization

A new eucaryotic expression system of recombinant PoIFN-γ was also constructed so that the denaturalization and refolding process is avoided. The target gene coding PoIFN-γ2 mature protein was subcloned into expression vector, transformed into Pichia pastoris, The His〓 Mut〓 phenotype transformants were screened, fermented in flasks and induced by 1％methanol. The expressed product in culture solution and sedimentation have antiviral activity on VSV and immunological activity proved by indirected immunofluorescence antibody and Dot blotting assay.

为了构建可对表达产物修饰、加工的真核表达系统，使表达产物具有自然干扰素的活性，无需变性、复性，我们将PoIFN-γ2成熟蛋白基因连接酵母整合表达载体，电转化毕赤酵母，筛选了His〓Mut〓表型转化子，摇瓶培养，1％甲醇诱导表达，培养上清和菌体裂解上清均有抗VSV病毒活性，经间接免疫荧光抗体检测和Dot blotting鉴定，重组酵母菌诱导表达产物具有免疫活性。

The main pathology histology change included liver denaturalization, necrosis, spleen giant cell hyperplasia, lymphocyte necrosis, lung and kidney microthrombus form, small intestine edema, microvascular hyperemia, cerebrum neuronophagia and satellitosis.

主要病理组织学变化为：肝变性、坏死，脾巨细胞增生、部分淋巴细胞坏死，肺充血，肾间质血管微血栓形成，小肠绒毛结构被破坏，固有层水肿，间质微血管充血，大脑噬神经现象和卫星化现象等。

The experimentation was making-know: the expand- ing of straw make for it denaturalization, and it can improve the assimilating rate of usefulness substance in the straw, the thick fibre is marked; The grain of straw is favorable for it is used sufficiently, but it have to use the grain material to felt straw; When it is comminuted to 16 mesh, moisture 35%~38%, straw 14%, the Expa...

研究表明，秸秆的膨化有助于物料的变性，能提高秸秆中的有用物质特别是粗纤维的吸收率；秸秆的颗粒化，有利于秸秆的充分利用，但是必须以谷物料原料作为粘结材料；秸秆的细化程度为16目、水分含量为35％-38％、秸秆干重含量为14％时，膨化效率较高；膨化后的颗粒饲料，有较好的抗压强度，有利于贮藏运输以及商品经营。

Results: The animal experimental model indicates the facial nerve' s bone canal fracture can make the facial nerve hyperaemia, strut, and putrescence, denaturalization and with the Schwann cell and the cell become more and more, taking on the restore evidence.

结果 动物实验表明：面神经骨管骨折后，面神经发生充血肿胀，并出现脱髓鞘、变性及坏死的变化，伴随神经损伤的同时出现巨噬细胞和施万细胞增生肥大及纤维母细胞的增生，呈现修复迹象。

As support, Agarose can benot only used in normal condition, but also in denaturalization (for example, 6 mol/Lhydrochloric acid carbamidine or 8 mol/L urea). Herein, the preparation of IMACmatrixes were conducted using agarose as support, carboxymethylated aspartate as chelating ligand and Co~(2+) and Ni~(2+) as chelating center ions.

本实验用琼脂糖作为基质，选用四配位，与金属离子有较强作用力的羧甲基天冬氨酸为配基，以Co~(2+)、Ni~(2+)作为螯合金属离子，合成了一系列金属螯合亲和色谱填料，并对其蛋白质分离性能进行了研究。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。