查询词典 deletion

Results The GSTM1 gene deletion rate in cataract group was 53.25% and that in the control group was 46.05%, they being not significantly different statistically (χ2=0.750, P＞0.05, OR=0.75). GSTM1 gene deletion rate in the subcapsular epithelial cells of 20 cases was basically consistent with that in blood cells.

结果 白内障组GSTM1基因缺乏率为53.25%，对照组为46.05%，两组间差异无显著性(χ2=0.750， P＞0.05， OR=0.75)。20例老年性白内障晶体上皮细胞内GSTM1基因检测与外周血细胞检测结果一致(P＜0.01)。

With fixed size of data table, computational speedup factor increases with increament of tolerance error. With same tolerance error, speedup factor of node deletion approach is higher than that of global deletion.

当数据表数据容量一定时，计算的加速比随控制容差的增加而增加，在相同的容差条件下，节点删除算法比整体删除算法加速效果明显。

In such a case, the page should be undeleted and listed on wikipedia:votes for deletion for a period of five days following the deletion of the user and user talk page.

对于管理员来说，您不能删除您的用户页或对话页，而必须要列在这里，以避免系统管理员通过删除页面来隐藏负面的评论，而其他人不能。

Methods S. epidermidis atlE gene expression level at different phases was detected by RT-PCR, and the accumulation of extracellular DNA at different phases was assessed by spectrophotometric measurements of light absorbance by DNA; DNase Ⅰ was used to study the function of extracellular DNA in biofilm formation and primary attachment. The effects of atlE gene deletion on initial adherent capacity, biofilm formation and extracellular DNA release were studied by construction of atlE deletion mutant via homologous recombination.

采用RT-PCR法检测表皮葡萄球菌atZE基因在不同时期的表达水平，用紫外分光光度计检测DNA的方法检测了相应时期的胞外DNA的释放量，并用DNA酶研究表皮葡萄球菌胞外DNA在生物膜形成和起始黏附中的作用；采用ρBT2质粒同源重组敲除的方法构建了表皮葡萄球菌1457的atlE基因突变株，研究atlE基因敲除突变对起始黏附能力、生物膜形成及胞外DNA释放能力的影响。

Six different deletion and insertion mutants of BBSV-X were constructed and these were inoculated to Chenopodium amaranticolor. The preliminary results show that the sat-RNA could enhance symptom of BBSV-X and its insertion mutants on Chenopodium amaranticolor, but the pathogenicity of the CP deletion mutants was reduced.

构建了BBSV-X基因组6个缺失和插入突变体，将它们的体外转录物单独或与sat-RNA一起混合接种苋色藜，初步实验结果表明，sat-RNA能增强全长BBSV-X基因组及含全长基因组的突变体对苋色藜所致的症状，但减轻CP缺失突变体的致病性。

This work might lead to develop many better methods or means of preventing and controlling dysenteric diarrhea.To elucidate the role of H-NS protein in bacterial physiology, the hns gene insertion and deletion mutants were constructed with RecA and Red recombination system. Then, growth curve and invasion ability of wild strain and deletion mutant were studied.

为了更深入的了解hns基因在细菌生命活动中所起的作用，我们首先利用RecA重组系统构建了痢疾杆菌2457T hns基因的插入突变体；随后，对基于Red重组系统的痢疾杆菌2457T缺失突变体的构建方法进行了摸索并成功地敲除了hns、hdeA、hdeB、yhiE和yhiF 5个基因，研究表明延长同源臂的长度可以明显提高同源重组的效率，缩短构建缺失突变体所需的时间。

The resulting strain, designated YES2MTM1, was transformed with a yeast genomic library. Transformants lost the plasmid overexpressing MTM1 after 5-FOA treatment. Yeast strains able to grow on nonfermentable carbon source with MTM1 deletion and overexpression of some DNA fragments were picked up and candidate suppressor genes were identified. Overexpression of five genes were identified to be able to rescue the growth defect on nonfermentable carbon source. The study will provide reference for MTM1 gene function and screening for suppressor of genes whose deletion result in irreversible damage.

为了避免MTM1缺失造成的不可逆损伤，在野生型酵母中先转入带有MTM1 基因的质粒，再敲除染色体上的MTM1 基因，随后转入基因组文库，再利用药物5-氟乳清酸(5-FOA)迫使细胞丢失表达MTM1基因的外源质粒，再筛选能在非发酵培养基上生长的转化子，通过这种方法筛选发现，POR2等5个基因的过表达可以挽救MTM1 基因缺失造成的非发酵培养基上的生长缺陷，为深入了解MTM1基因的功能提供了线索，对筛选其他造成不可逆损伤的突变基因的抑制基因提供了一条可行的研究思路。

Ninety-three patients with forty-two azoospermia and fifty-one severe oligozoospermia were recruited in present study. Y chromosome microdeletions were screened by detection of four locus SY84, SY134, SY255 and SPGY1 gene spaning the AZFa, AZFb and AZFc subregions of Y chromosome. The total incidence of Y microdeletions was 16%( 15/93 )in present study. Eight men in 42 patients with azoospermia had Y microdeletions, of whom 6 were AZFc deletion and 2 were AZFb deletion.

通过对93例男性不育（42例无精症和引例严重少精症）患者，Y染色体 SPGY基因、AZFa－SY84、AZFb－SY134和 AZFc－SY255位点缺失情况的研究，我们发现男性不育患者 Y染色体微缺失的发生率为 16％（ 5旧3人 42例无精子症患者中，有8例存在Y染色体微缺失，其中6例为AZF。

A set of spectific primers was syn thesized according to HBV DNA sequence of Chinese strain, the whole X region was amplified by PCR method from the serum of 9 patients with chronic HBV infection , and then the PCR products were subcloned into pGEM Teasy vectors. Clones were randomly selected to be sequenced. Comparison of the cloned sequence was made to find the difference. After being compared, each sequence of selected clones is o f difference. The point mutation scattered through X region. Deletion mutations were detected in 19 clones of 37(51.4%), which caused different carboxyl endings of X protein. There is a hot region (after 123 aa code) where deletion mutation frequently happens.

以中国株HBV基因序列为依据，设计特异性多聚酶链反应引物，自9例慢性HBV感染患者血清中扩增HBV X基因，克隆入pGEM Teasy质粒，随机挑选克隆进行D NA测序以确定病毒的变异程度。37例测序结果提示来源于不同患者HBV X基因序列高度保守，但每个序列均不一致。X区除了存在广泛的碱基点替换突变外，序列的缺失突变占测序克隆总数的51.4％（19／37）；氨基酸缺失及移框突变多发生于123位氨基酸残基之后，可导致X蛋白多种羧基端形式。

The mutation rate was about 80% and 10 of the 22 deletion mutations were uncomplete deletion mutations, which had two amplified DNA bands less 509 bp.

在22例缺失突变病例中，有10例为不完全缺失突变即有低于外显子509 bp的扩增产物。

Sometimes we make snowballs and throw them at each other.That's very interesting.

我的朋友和我都喜欢堆雪人，有时打雪仗，那是多么有趣。

I love you the way yu are .

我真心爱你。