查询词典 deletion list

This work might lead to develop many better methods or means of preventing and controlling dysenteric diarrhea.To elucidate the role of H-NS protein in bacterial physiology, the hns gene insertion and deletion mutants were constructed with RecA and Red recombination system. Then, growth curve and invasion ability of wild strain and deletion mutant were studied.

为了更深入的了解hns基因在细菌生命活动中所起的作用，我们首先利用RecA重组系统构建了痢疾杆菌2457T hns基因的插入突变体；随后，对基于Red重组系统的痢疾杆菌2457T缺失突变体的构建方法进行了摸索并成功地敲除了hns、hdeA、hdeB、yhiE和yhiF 5个基因，研究表明延长同源臂的长度可以明显提高同源重组的效率，缩短构建缺失突变体所需的时间。

The resulting strain, designated YES2MTM1, was transformed with a yeast genomic library. Transformants lost the plasmid overexpressing MTM1 after 5-FOA treatment. Yeast strains able to grow on nonfermentable carbon source with MTM1 deletion and overexpression of some DNA fragments were picked up and candidate suppressor genes were identified. Overexpression of five genes were identified to be able to rescue the growth defect on nonfermentable carbon source. The study will provide reference for MTM1 gene function and screening for suppressor of genes whose deletion result in irreversible damage.

为了避免MTM1缺失造成的不可逆损伤，在野生型酵母中先转入带有MTM1 基因的质粒，再敲除染色体上的MTM1 基因，随后转入基因组文库，再利用药物5-氟乳清酸(5-FOA)迫使细胞丢失表达MTM1基因的外源质粒，再筛选能在非发酵培养基上生长的转化子，通过这种方法筛选发现，POR2等5个基因的过表达可以挽救MTM1 基因缺失造成的非发酵培养基上的生长缺陷，为深入了解MTM1基因的功能提供了线索，对筛选其他造成不可逆损伤的突变基因的抑制基因提供了一条可行的研究思路。

Ninety-three patients with forty-two azoospermia and fifty-one severe oligozoospermia were recruited in present study. Y chromosome microdeletions were screened by detection of four locus SY84, SY134, SY255 and SPGY1 gene spaning the AZFa, AZFb and AZFc subregions of Y chromosome. The total incidence of Y microdeletions was 16%( 15/93 )in present study. Eight men in 42 patients with azoospermia had Y microdeletions, of whom 6 were AZFc deletion and 2 were AZFb deletion.

通过对93例男性不育（42例无精症和引例严重少精症）患者，Y染色体 SPGY基因、AZFa－SY84、AZFb－SY134和 AZFc－SY255位点缺失情况的研究，我们发现男性不育患者 Y染色体微缺失的发生率为 16％（ 5旧3人 42例无精子症患者中，有8例存在Y染色体微缺失，其中6例为AZF。

A set of spectific primers was syn thesized according to HBV DNA sequence of Chinese strain, the whole X region was amplified by PCR method from the serum of 9 patients with chronic HBV infection , and then the PCR products were subcloned into pGEM Teasy vectors. Clones were randomly selected to be sequenced. Comparison of the cloned sequence was made to find the difference. After being compared, each sequence of selected clones is o f difference. The point mutation scattered through X region. Deletion mutations were detected in 19 clones of 37(51.4%), which caused different carboxyl endings of X protein. There is a hot region (after 123 aa code) where deletion mutation frequently happens.

以中国株HBV基因序列为依据，设计特异性多聚酶链反应引物，自9例慢性HBV感染患者血清中扩增HBV X基因，克隆入pGEM Teasy质粒，随机挑选克隆进行D NA测序以确定病毒的变异程度。37例测序结果提示来源于不同患者HBV X基因序列高度保守，但每个序列均不一致。X区除了存在广泛的碱基点替换突变外，序列的缺失突变占测序克隆总数的51.4％（19／37）；氨基酸缺失及移框突变多发生于123位氨基酸残基之后，可导致X蛋白多种羧基端形式。

The mutation rate was about 80% and 10 of the 22 deletion mutations were uncomplete deletion mutations, which had two amplified DNA bands less 509 bp.

在22例缺失突变病例中，有10例为不完全缺失突变即有低于外显子509 bp的扩增产物。

Using CCA, karyotype abnormalities were found in 18 (37.5%) of 48 cases, among which complex karyotypes were found in 4 (8.3%) cases, a deletion of chromosome 5/a deletion of the long arm of chromosome 5 (-5/5q-) in 5 (10.4%) cases,-7/7q- in 5 (10.4%) cases, trisomy 8 in 8 (16.6%) cases, 20q- in 2 (4.6%) cases, unconsistent translocations in 3 (6.2%) cases.

细胞遗传学分析检出染色体异常18例(37.5%)，其中复杂异常4例(8.3%)、5号染色体缺失或5号染色体长臂部分缺失5例(10.4%)、7号染色体缺失或7号染色体长臂部分缺失5例(10.4%)、8号染色体增加8例（16.6%）、20号染色体长臂部分缺失2例（4.6%）、不一致的易位3例（6.2%）。

Unctional study of the negative selection sites, possible Darwinian positiveselection sites and deletion mutants The aim of this study was to observe the role of negative selection sites,possible Darwinian positive selection sites and deletion fragments in FKN/CX3CR1 binding affinity, chemotaxis and adhesion.

KN／CX3CR1负选择位点、可能存在的达尔文正选择位点及缺失片段的体外功能研究观察负选择位点，可能存在的达尔文正选择位点及缺失片段对FKN／CX3CR1介导的功能的影响，应用定点突变技术构建不同的突变子，体外实验观察突变子对FKN／CX3CR1在亲合力、趋化功能和粘附功能上的影响。

The BORUI software belongs to the field of computer software technology, and is characterized by that after the BORUI software is mounted in computer, when the mourse is placed on the icon of an unopened file, one window can be automatically ejected, the file contents or preset contents can be displayed in the window, after the file is opened, the preset contents also can be looked up by means of software function; in the computer said BORUI software has several functions; inhibiting deletion, enciphering deletion function and making reference and comparison between various files with different types and different forms.

BORUI软件属于计算机软件领域，其特征在于：当计算机安装BORUI软件后，将鼠标放置在一个未打开的文件的图标上时会自动弹出一个窗口，窗口中可显视其文件内容或预先设定的内容；在文件打开后也可以通过软件功能查看其预先所设定的内容；通过软件功能可将计算机中不同类型不同格式的文件设定成为禁止删除；可为删除功能加密，为删除功能加密后，只能通过输入正确的密码才可以将文件删除；通过软件的功能可以在文件中的文字与文字、图像与图像、视频与视频、动画与动画、音频与音频等不同类型不同格式的各种文件之间进行参照与对比。

The cybrids containing 0% and 85% of mtDNA with 4977-bp deletion as an experimental system were used as an experimental system to investigate the effects of high proportion of 4977-bp mtDNA deletion on apoptotic behaviors of human cells.

我乃利用此技术所制造的细胞质融合细胞株，探讨高百分比4977-bp断损突变对细胞凋亡的影响，希望藉此可以更进一步了解粒线体脑肌病之致病机转。

Deletion of attribute references, subscriptions and slicings is passed to the primary object involved; deletion of a

对于属性引用，下标和片断的删除会作用到相关的主元对象，对片断的删除一般等价于对该片断赋予相应

So hunting for monsters begins, Agent Chewer follows in the track of monsters that leave a lot of cheese bits.

在妖怪们开始狩猎的时候，Agent Chewer 从妖怪们残留的干酪渣跟踪它们。

According to the finite points within region, divide the region into trigonal section network. The DEM has made up of continuous trigonal section.

根据区域的有限个点集将区域划分为相等的三角面网络，数字高程有连续的三角面