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The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法,研究丘脑网状核(nucleus reticularis thalami,RT)中γ-氨基丁酸(gamma-amino butyric acid ,GABA)能神经元、一氧化氮(nitrogen monoxidum,NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate,cAMP)及中缝背核(nucleus raphes dorsalis,DRN)至RT的5-羟色胺(5-hydroxytryptamine,5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP,5μg),注射当天睡眠时间略有减少,第二日睡眠时间显著减少,觉醒时间明显增多,第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后,与生理盐水组比较,睡眠时间增加,觉醒时间减少;而RT内微量注射L-谷氨酸(glutamic acid, L-Glu, 0.2μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline,BIC,1.0μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen,BAC,1.0μg)后,产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg,0.5μg)后,与生理盐水组对比,睡眠时间略有减少,但无显著性意义;而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside,SNP,0.2μg)后可明显增加觉醒时间,缩短睡眠时间;微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine,L-NNA,2.0μg)后,引起睡眠时间增多,觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用;RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后,与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠,其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate,MOR,1.0μg)后与生理盐水组对比,睡眠时间减少而觉醒时间增加; RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride,NAL,1.0μg)后与生理盐水组比较,睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后,与生理盐水组对比,原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较,睡眠时间增多而觉醒时间减少;RT内微量注射亚甲蓝(methylene blue,MB,1.0μg)后,与NS组比较,睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 0.2μg)比较,睡眠时间减少,觉醒时间增多;大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射二甲基麦角新碱(methysergide, MS, 1.0μg )后,与对照组(DRN注射L-Glu 0.2μg,双侧RT注射NS 1.0μg)比较,睡眠时间增多,觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine,PCPA,10μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 1.0μg)比较,睡眠时间增多,觉醒时间减少;大鼠DRN内微量注射PCPA(10μg),产生睡眠增多效应后,在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan , 5-HTP, 1.0μg )后,与对照组(DRN注射PCPA 10μg,双侧RT注射NS 1.0μg)比较,睡眠时间减少,觉醒时间增多。

The company launched the "three-saving" activities and focus on day-to-day work from the start, in the office take full advantage of natural illumination region and resolutely put an end to the "light" and "day light", to strengthen the internal management of water equipment maintenance, and resolutely put an end to " long flowing water ", make full use of network resources, the promotion of office automation and paperless office, a serious work ahead of time in front of the reasonable use of the material planning and arrangements, was to make good use of electricity, a piece of paper, a nut, a pin nails a pin, one inch wire.

该公司在开展&三节约&活动过程中注重从日常行为和工作入手,在办公区域充分利用自然光照,坚决杜绝&长明灯&和&白昼灯&,加强公司内部的用水设备维护管理,坚决杜绝&长流水&,充分利用网络资源,提倡办公自动化和无纸化办公,认真提前做好工作前的使用材料的合理计划和安排,用好一度电,一张纸、一个螺帽、一个脚钉、一个销子、一寸导线。

We opened the campus radio, teacher Zhan Ming-ying, and broadcasters, correspondents dedicated the beautiful audio-visual works with perseverance to everyone; we have compiled and published our own newspaper,"Journal of LQ Middle School"; our blackboard newspaper, and cultural fields are more standardized and regular ; we have organized the celebration of "Women's Day" and Teacher's Day, We organized faculty members to go out for inspection, carried out teachers and staffs Red song concerts; we held exchange activitives with No.5 Middle School of Anqing, Changling secondary schools, Taihu Dashi secondary school, LQ primary school, Bohu primary school; we held a school-based training courseware, organized all the teachers listened to local experts, Yang Jiedong's thematic reports, teacher Wei Shusheng's video report, organized the head teachers to listen to the national top video of the head teacher report , organized the teacher to Nanjing and Shanghai and Anqing Teachers College in groups for refresher training, seriously at all levels of teaching and research activities; officially formed a faculty basketball team and on behalf of LQ village took part in the county's National Day competition; teacher Zhu Hongmin and Xu Chunming represented our school to take part in "springs Cup" Table Tennis competition; we brought in the dance team of the county college for the old to perform; we held the graduation ceremony of the 2009 session; we hold regularly the reading and speaking contest for students, the English showing, the teachers Courseware competition, young and middle-class high-quality math and English classes Contest quality; we hold table tennis competition, successfully held the second session of the school track and field sports; General League branch, and students' union held activities to commemorate the earthquake in Wenchuan and commemorate teacher Li Jinhua , has organized fund-raising activities for many times; branch of the Praty, Representatives of the staffs, General branch of the League, and students union held a successful general election; Organized CDC of county and township hospitals for free medical examinations for students, extensively carried out the publicity of combating TB and the prevention and control and we have safely passed in 2009!

我们开通了校园广播,詹铭瑛老师和播音员、通讯员持之以恒地把精美的视听作品奉献给了大家;我们编辑出版了自己的报纸《凉泉中学报》;我们的板报和文化栏更加规范化、经常化;我们举办了三八节和教师节的庆祝活动,组织了教职工外出考察,开展了教职工红歌演唱会;我们举行了与安庆五中、长岭中学、太湖大石中学、凉泉小学、泊湖小学的校际交流活动;我们举行了校本课件制作培训、组织全体教师聆听了本土专家杨结东主任的主题报告、魏书生老师的录像报告、组织了班主任老师聆听全国十佳优秀班主任的录像报告、分批组织了老师到南京到上海到安庆师范学院进修培训、认真参与各级教研活动;正式组建了教工篮球队并代表凉泉乡参加了全县国庆比赛;朱红敏和徐春明老师代表学校参加了凉泉乡五四&清泉杯&乒乓球比赛;我们请来了县老年大学的舞蹈队来校演出;我们隆重举行了2009届毕业典礼;我们定期举行了学生读书演讲比赛、英语风采大赛、老师课件制作比赛、中青年数学优质课和英语优质课大赛;我们分校区举办了全校乒乓球比赛,成功举办了第二届全校田径运动会;团总支和学生会举行了纪念汶川大地震的活动、纪念李精华老师的活动、多次组织了募捐活动;党支部、教代会、团总支和学生会成功举行了换届选举;组织县疾控中心和乡卫生院免费为学生体检,广泛开展了防治结核病和防控甲型流感的宣传,平安地度过了2009年!

Pening range breakout is effective after an inside day that has a smaller daily range than the previous four days (IDnr4 in Figure 1 ) and after any day that has a daily range less than the previous six days (NR7) whether an inside day or not.

RB这一现象发生在IDnr4或者NR7之后常常是有效的。IDnr4是指振幅小于前面4天的孕线形态。NR7是指振幅小于前面6天的形态。

Thanksgiving day is actually very much a reformed/protestant day, catholics (I suppose you call them papists) don't celebrate this day at all.

整体而言,还是要写感恩邮件,不过最好对非美国的客户写的邮件,感恩节这个节日强调要少一些。

Keeping this in mind, a study was undertaken to determine the effect of CA on early growth of mung bean (Phaseolus aureus=Vigna radiata), morphogenetic response in hypocotyl cuttings of mung bean, and changes in the activities of some associated enzymes – proteases, polyphenol oxidases, and peroxidases – and endogenous total phenolics at root initiation (third day; 48 h; appearance of root primordia; RI), root expression (fifth day; 120 h; appearance of roots; RE), and post-expression (seventh day; 168 h; PE) stages of rhizogenesis.

保持这一点,一项研究,以确定影响钙对早期生长绿豆( phaseolus金黄色葡萄球菌=豇豆拉迪雅塔),形态发生反应,下胚轴插条绿豆,和变化,在活动的一些相关的酶-蛋白酶,多酚氧化酶,过氧化物酶-和内源性总酚的从根本上启动(第三天; 4 8小时;外观根原基;里),根的表达(第五天; 1 20改为H级;外观根;转口),和后表达(第七天; 168改为H级; PE )的阶段rhizogenesis 。

In summer-autumn,2002,we developed treat-patient-free activity:on " Army Day "、" Teacher Day " and " National Day ",the patient may be free of registration fee,derate 10% of medicines'fee.In Aug.2002,we established health school for citizens:every month a famous expert makes lectures on popular science,for free of surveying blood sugar and blood pressure on the spot,at the same time,students may gain free membership certificate of healthy school and enjoy more preferential treatment.

以人性化的服务,打造&人文关爱工程&。2002年夏秋季开展人文关爱的系列义诊活动,具体为&建军节&、&教师节&和&国庆节&开展为患者服务,患者就诊免交挂号费、药品费减免10%。2002年8月在省内首创为市民免费讲座的健康学校,每月由一名知名专家就医学知识进行科普讲座,现场免费对听众测血糖、量血压,同时,听课者免费获得健康学校会员证,会员来院就诊享有多项优惠待遇。

Results In the group of normal rats with partial hepatectomy, immunohistochemistry staining indicated that the expression of HGF and C-met increased to get the peak point after partialhepatectomy 12 hours and 3 days respectively, and HGF maintained at the high level to the 7th day, then decreased gradually, finaly approched to the level of pro-operation at 14th day, but C-met fell sharply,and declined to the the level of pro-operation at the 14th day.

结果 免疫组织化学显示,正常肝部分切除组,HGF表达于术后12 h达高峰,并维持高峰平台,7 d后逐渐降低,于14 d接近术前水平;C-met术后迅速上升,3 d达到高峰,而后逐步下降,14 d降回术前水平。

With initial immunization at day 70 and a booster at day 98, the growth performance of the yellow-feathered broiler chickens exhibited a stage-wise pattern up to slaughter at the end of the 49-day postinoculation trial period.

在经过首免,28天后的二免,到119天龄屠宰间,黄羽肉鸡的生长性能表现出阶段性的特点。

I cite a case, price of this word contest is as high as tumor 16 yuan are clicked every time, one day clicks the word of 1000 is then 16 thousand yuan everyday, lead the 1% words that will calculate by changeover (conservative estimation is inside this limits), so a day of 1000IP the word has 10 people is duped, 16 thousand yuan of affirmation of a day want these 10 people to come namely burden, so be equivalent to you treat disease most most the injustice money that the most conservative affirmation should give is the least 1600 yuan (affirmative of course and more than this, the person that just says to see a doctor here also should do a good turn cost of price giving contest, sweat).

我举个例子,肿瘤这个词竞价高达16元每次点击,一天点击1000的话那就是1.6万元每天,按转换率1%来算的话,那么一天1000IP个话有10个人是上当的,一天的1.6万元肯定就是要这10个人来负担,那么相当于你去看一次病最最最保守肯定要出的冤枉钱最少是1600元(当然肯定不止这个,我这里只是说看病的人也要帮忙出竞价费,汗)。

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相关中文对照歌词
Day By Day
Day After Day (The Show Must Go On)
Day By Day
Day In Day Out
Day After Day
Day To Day Routine
Day By Day
Day In, Day Out
Day Upon Day
Day In Day Out
推荐网络例句

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候,里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色,卵在发育96h后卵基本全部孵化。

There was a conflict between plebs and patricians in ancient Rome in 494BC.

在公元前494年,罗马发生了一次平民反对贵族的斗争。