查询词典 daf

Methods: Genes of MCP and DAF were connected by 10aa coding sequence of enriching Gly and Ser. Then insert into pcDAN3.1 vector, constituting pcDNA-MD3 expression plasmid of hMCP-DAF fusion gene. The genes were transformed by liposome into the pig endothelial cell; human serum handles masculine cloning, identify the function that restrain breaking of person alexin.

将MCP、DAF两个基因以富含Gly和Ser的10aa编码序列作铰链串接，并接入表达载体pcDNA 3.1中，构成含hMCP-DAF的真核表达质粒(pcDNA-MD3)；以脂质体转染猪内皮细胞，人血清处理阳性克隆细胞，鉴定抑制人补体溶破的功能；应用显微注射将线性基因导入猪受精卵；PCR检测导入基因整合率。

However, there is some defects in the properties of DAF, such as its limited decay-accelerating activity and lacking of cofactor activity of I factor, which limited its usage in clinic.

然而， DAF仍存在某些缺陷，比如其有限的衰变加速能力并缺乏Ⅰ因子的辅因子活性限制了DAF的临床应用，其为部分病原微生物受体的角色对异种移植有潜在危险。因而对DAF进行改造是一种较好的选择，也是当务之急。

That synergistic action was not performed through pathway of IL-2 secretion, and need no cross-link between DAF and CD3.Hydrophilicity, solvent accessibility, secondary structure and B cell epitopes of DAF were predicted by protein surface analysis and molecular model of SCR3 and SCR4 in DAF which was constructed with computer software based on theory of homology modeling.

首次运用同源蛋白质结构预测的理论和*国家自然科学基金项目(39500031)：全军"九五"医药卫生科研基金资助项目方法建立DAF中SCR3和SCR4的三维分子模型，并对DAF的亲水性、可及性、二级结构及B细胞表位进行预测。

Objective: The genes were connected basing on obtain gene of MCP and DAF of liver and fetal marrow of Chinese adult and then design mammiferous vector of the hMCP-DAF fusion gene. Finally transfer hMCP-DAF fusion gene to the early embryo of jinhua''s pig.

目的 在获得中国成人肝脏、胎儿骨髓MCP、DAF基因克隆的基础上，进一步将两个基因串接，并构建hMCP-DAF融合基因的哺乳动物表达载体，筛选出具表达活性的hMCP-DAF并导入金华猪早期胚胎。

It was confirmed that the mutation of nonconservative animo acids in DAF could improve the activities of DAF and the positive charges on the surface of DAF were necessary for its binding with

并证实了DAF分子的非保守性氨基酸突变可提高其活性。

By comparing the amino acids sequences of SCRs of different regulators of complement activation and homologous structure units of DAF in different species, the conservative and nonconservative amino acids in DAF were determined. The possible activity sites were analysed and mutation amino acids sites to construct the mutant library were defined.3. Constructing and evaluating of yeast display mutant library on DAF scaffold.

SCRs多重序列比较及DAF突变位点的选择通过对补体调控蛋白家族成员、不同种属DAF分子的同源结构单位SCR的氨基酸序列进行多重序列比较，确定了DAF分子中的保守性氨基酸和非保守性氨基酸，并根据已有的实验结果，对DAF分子可能活性位点进行了分析，确定了构建酵母细胞表面呈现库的突变氨基酸位点。

Most normally fluent speakers become highly disfluent when exposed to DAF and many stutterers experience decreased stuttering under DAF.

绝大多数说话流畅的正常人当暴露在DAF的环境中时，说话会变得非常结巴；而许多口吃者在DAF的环境中说话时，口吃却会减轻。

The result showed that three mAbs could bind onto DAF displayed on the surface of yeast.

结果显示在酵母细胞表面能检测到与以上单抗结合的DAF分子，说明了DAF分子在酵母细胞表面呈现，并且呈现的DAF分子能正确折叠，形成了天然的构象。

Molecular model and surface analysis of DAF place a base for studies on structure and function of SCRs, construction of DAF mutation and new form of DAF with multiple function.

三维模型的建立及分子表面特性的分析为研究SCRs的结构与功能、构建DAF突变体及新型多功能DAF分子奠定了基础。

The purified DAF and anti-DAF McAbs obtained, as well as assays established in this project facilitate the researcher in same fields.

本课题研究所获得的DAF纯品、抗DAF单抗以及所建立的制备和检测方法为同行开展DAF的相关研究提供了有利条件。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。