查询词典 cytoplast

The paper mainly reviewed several factors on influencing somatic cloning of pig , which involves in cytoplast recipients、 donor cells、micromanipulation、activation and reconstituted embryo transfer .

本文主要综述了影响体细胞克隆猪的几个因素，涉及胞质受体、供核细胞、显微操作、激活以及重组胚移植。

In autumn,there were GTH1 cells and GTH2 cells in GTH cells. A large number of secretory granules in GTH1 cells were circumnuclear; In GTH; cells cytoplast,there were two types of secretory granules,small circle and large circle, in small number.Structure of STH cells had no obvious changes.

秋季，在GTH细胞中发现GTH_1和GTH_2两种细胞，GTH_1细胞具有大量的分泌颗粒，并呈围核分布，GTH_2细胞胞质中具有数量较少的小圆形分泌颗粒和大圆形颗粒；STH细胞的结构无明显变化。

In winter,nuclear of GTH cells were elliptical and a small number of secretory granules scattered around nucleolus. Number of secretory granules in STH cells were relatively small, nucleolus distorted, activity of synthesis and secretion decreased. Secretory granules in TSH cells cytoplast were relatively small.

冬季，GTH细胞核为椭圆形，有少量的分泌颗粒散布在细胞核的周围；STH细胞分泌颗粒数量较少，胞核变形，合成和分泌活动比较微弱；TSH细胞胞质中的分泌颗粒相当多。

It is concluded that (1) there are no relationships between fusion rate and relativeness of the recipient cytoplasm to nucleus donor cells,(2) cytoplast of the goat MII oocyte can support the preimplantation development of SCNT embryos reconstructed with nucleus from other species,(3) the blastocyst rate of close relative inter-species SCNT embryos is higher than that of distant relative inter-species SCNT embryos.

由此结果得出以下结论：(1)山羊M II期卵母细胞胞质与供核细胞之间的亲缘性不影响两者的融合率；(2)山羊M II期卵母细胞的胞质能支持异种间体细胞核移植胚的着床前发育；(3)亲缘关系近的种间核移植胚的囊胚发育率高于亲缘关系远的种间核移植胚的。

Although the ability to reprogramming of Kun Ming white mouse oocytes cytoplast is not effectively, reprogramming ability has improved when cystoplasts from oocytes and embryos work together.

昆明白小鼠卵母细胞胞质对体细胞核的重新编程能力较弱，而当其与正常受精卵的胞质共同作用时可较好地改善对体细胞的重新编程能力。

In winter,nuclear of GTH cells were elliptical and a small number o f secretory granules scattered around nucleolus. Number of secretory granules in STH cells were relatively small, nucleolus distorted, activity of synthesis and secretion decreased. Secretory granules in TSH cells cytoplast were relatively small.

冬季，GTH细胞核为椭圆形，有少量的分泌颗粒散布在细胞核的周围；STH细胞分泌颗粒数量较少，胞核变形，合成和分泌活动比较微弱；TSH细胞胞质中的分泌颗粒相当多。

Over the last 10 years, antisense transgenic plants have been used as tools to address this and have revealed some unexpected findings. In this article, chloroplast tpi gene from spinach and tandem genes of chloroplast tpi gene from spinach and cytoplast fba gene from rice were transferred into filamentous, heterocystour cyanobacterium Anabaena sp.

将菠菜叶绿体tpi和水稻胞质fba的串联基因连接在光诱导的高效启动子PpsbA下游，克隆入pDC-08中构建了既能在大肠杆菌又能在蓝藻中表达的穿梭表达载体pDC-AT，并通过三亲接合转移转入鱼腥藻7120。

After treatment in M2〓 medium containing 3% sucrose, a swelling around the metaphase chromosomes could be easily found in perivitelline space and, moreover, the area of spindle, becoming transparent and taking a shape of"∞"and"O", could be well distinguished from other cytoplast.

在本研究中，采用含3％蔗糖的M2〓操作液（3SCOM）处理卵母细胞，可使纺缍体部分变为透明并呈"〓"或"O"形状，这样可使其明显区别于周围的细胞质。

Microcells were produced by centrifugation of micronucleated A9 (neo12) cells in Percoll density gradient containing 20 mg/L Cytochalasin B at 39 000 g. The resulting mixture of microcells, whole cells, karyoplasts and cytoplast fragments was filtered through 8 μm and 5 μm size membrane pores sequentially to obtain pure preparation of microcells.

微核化的细胞在含有20 mg/L细胞松弛B的Percoll密度梯度介质中，经39 000g高速离心后，包含微细胞、完整细胞、细胞核和细胞碎片的混合液，依次通过8 μm和5μm孔径的滤膜过滤后可获得纯化的微细胞溶液。

But expression of LT in the plant cytoplast had not reported. A subunit and B subunit from strongly nosogenetic bacilli K88ac+ was cloned respectively .We constructed two kinds of bivalent plant expression vectors.

本实验研究的目的是将从K88ac+强致病菌株中克隆的LTA、LTB基因，构建成两种双价植物高效表达载体，其中一种是将LTA、LTB基因单独构建到两个表达框。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。